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Volume 61, Issue 5

gen. nov., sp. nov., a new member of the family Free

Abstract

A novel endophytic actinomycete, designated strain 202201, was isolated from an root collected from the mangrove reserve zone in Hainan Province, China. Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain 202201 fell within the family . The strain formed an extensively branched substrate mycelium, which carried uneven warty-surfaced spores. Cell walls of strain 202201 contained -diaminopimelic acid and xylose, mannose, arabinose, ribose and glucose were detected as whole-cell sugars. The acyl type of the cell-wall polysaccharides was glycolyl. The major menaquinones were MK-9(H), MK-9(H), MK-9(H) and MK-10(H). The polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol mannoside and phosphatidylserine. The major cellular fatty acids were 10-methyl-C, iso-C, iso-C and Cω8. The DNA G+C content was 72.3 mol%. On the basis of the morphological and chemotaxonomic characteristics, phylogenetic analysis and characteristic patterns of 16S rRNA gene signature nucleotides, strain 202201 ( = CGMCC 4.5597 = DSM 45430) represents a novel species of a new genus within the family , for which the name gen. nov., sp. nov. is proposed.

  • Published Online:
Funding
This study was supported by the:
  • Chinese National Programs for High Technology Research and Development (Award ITBBZD0753 and 2007AA09Z415)
  • National High Technology Development Project (Award 2007AA021307)
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2011-05-01
2024-04-26
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References

  1. Ara I., Kudo T. 2006; Three novel species of the genus Catellatospora, Catellatospora chokoriensis sp. nov., Catellatospora coxensis sp. nov. and Catellatospora bangladeshensis sp. nov., and transfer of Catellatospora citrea subsp. methionotrophica Asano and Kawamoto 1988 to Catellatospora methionotrophica sp. nov., comb. nov.. Int J Syst Evol Microbiol 56:393–400 [View Article][PubMed]
     [Google Scholar]
  2. Ara I., Kudo T. 2007a; Luedemannella gen. nov., a new member of the family Micromonosporaceae and description of Luedemannella helvata sp. nov. and Luedemannella flava sp. nov.. J Gen Appl Microbiol 53:39–51 [View Article][PubMed]
     [Google Scholar]
  3. Ara I., Kudo T. 2007b; Krasilnikovia gen. nov., a new member of the family Micromonosporaceae and description of Krasilnikovia cinnamomea sp. nov.. Actinomycetologica 21:1–10 [View Article]
     [Google Scholar]
  4. Ara I., Bakir M. A., Kudo T. 2008a; Transfer of Catellatospora koreensis Lee et al. 2000 as Catelliglobosispora koreensis gen. nov., comb. nov. and Catellatospora tsunoense Asano et al. 1989 as Hamadaea tsunoensis gen. nov., comb. nov., and emended description of the genus Catellatospora Asano and Kawamoto 1986 emend. Lee and Hah 2002. Int J Syst Evol Microbiol 58:1950–1960 [View Article][PubMed]
     [Google Scholar]
  5. Ara I., Matsumoto A., Bakir M. A., Kudo T., Omura S., Takahashi Y. 2008b; Pseudosporangium ferrugineum gen. nov., sp. nov., a new member of the family Micromonosporaceae . Int J Syst Evol Microbiol 58:1644–1652 [View Article][PubMed]
     [Google Scholar]
  6. Asano K., Kawamoto I. 1986; Catellatospora, a new genus of the Actinomycetales . Int J Syst Bacteriol 36:512–517 [View Article]
     [Google Scholar]
  7. Cardinali G., Liti G., Martini A. 2000; Non-radioactive dot-blot DNA reassociation for unequivocal yeast identification. Int J Syst Evol Microbiol 50:931–936[PubMed] [CrossRef]
     [Google Scholar]
  8. Castiglione F., Lazzarini A., Carrano L., Corti E., Ciciliato I., Gastaldo L., Candiani P., Losi D., Marinelli F., Selva E. 2008; Determining the structure and mode of action of microbisporicin, a potent lantibiotic active against multiresistant pathogens. Chem Biol 15:22–31 [View Article][PubMed]
     [Google Scholar]
  9. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
     [Google Scholar]
  10. Couch J. N. 1950; Actinoplanes, a new genus of the Actinomycetales . J Elisha Mitchell Sci Soc 66:87–92
     [Google Scholar]
  11. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–789 [View Article]
     [Google Scholar]
  12. Felsenstein, J. (2008). phylip (phylogeny inference package) Version 3.68, Department of Genome Sciences, University of Washington, Seattle, USA.
  13. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
     [Google Scholar]
  14. Goodfellow M. L., Stanton J., Simpson K. E., Minnikin D. E. 1990; Numerical and chemical classification of Actinoplanes and some related actinomycetes. J Gen Microbiol 136:19–36 [CrossRef]
     [Google Scholar]
  15. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. 1974; Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24:54–63 [View Article]
     [Google Scholar]
  16. Gregersen T. 1978; Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol 5:123–127 [View Article]
     [Google Scholar]
  17. Hong K., Gao A. H., Xie Q. Y., Gao H., Zhuang L., Lin H. P., Yu H. P., Li J., Yao X. S. et al. 2009; Actinomycetes for marine drug discovery isolated from mangrove soils and plants in China. Mar Drugs 7:24–44 [View Article][PubMed]
     [Google Scholar]
  18. Inahashi Y., Matsumoto A., Danbara H., Ōmura S., Takahashi Y. 2010; Phytohabitans suffuscus gen. nov., sp. nov., an actinomycete of the family Micromonosporaceae isolated from a plant root. Int J Syst Evol Microbiol 60:2652–2658 [View Article]
     [Google Scholar]
  19. Jones K. L. 1949; Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. J Bacteriol 57:141–145
     [Google Scholar]
  20. Kane W. D. 1966; A new genus of the Actinoplanaceae, Pilimelia, with a description of two species, Pilimelia terevasa and Pilimelia anulata . J Elisha Mitchell Sci Soc 82:220–230
     [Google Scholar]
  21. Kelly K. L. 1964 Inter-Society Color Council–National Bureau of Standards Color Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office;
     [Google Scholar]
  22. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
     [Google Scholar]
  23. Koch C., Kroppenstedt R. M., Rainey F. A., Stackebrandt E. 1996; 16S ribosomal DNA analysis of the genera Micromonospora, Actinoplanes, Catellatospora, Catenuloplanes, Couchioplanes, Dactylosporangium, and Pilimelia and emendation of the family Micromonosporaceae . Int J Syst Bacteriol 46:765–768 [View Article][PubMed]
     [Google Scholar]
  24. Krasil’nikov N. A. 1938 Ray Fungi and Related Organisms– Actinomycetales Moscow: Izdatel’stvo Akademii Nauk SSSR;
     [Google Scholar]
  25. Kroppenstedt R. M. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series no. 20) pp. 173–199 Edited by Goodfellow M., Minnikin D. E. New York: Academic Press;
     [Google Scholar]
  26. Kudo T., Nakajima Y., Suzuki K. 1999; Catenuloplanes crispus (Petrolini et al. 1993) comb. nov.: incorporation of the genus Planopolyspora Petrolini 1993 into the genus Catenuloplanes Yokota et al. 1993 with an amended description of the genus Catenuloplanes . Int J Syst Bacteriol 49:1853–1860 [View Article][PubMed]
     [Google Scholar]
  27. Lechevalier M. P., Lechevalier H. A. 1980; The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy (Special Publication no. 6) pp. 227–291 Edited by Dietz A., Thayer J. Arlington, VA: Society for Industrial Microbiology;
     [Google Scholar]
  28. Lechevalier M. P., De Bièvre C., Lechevalier H. A. 1977; Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5:249–260 [View Article]
     [Google Scholar]
  29. Lechevalier M. P., Stern A. E., Lechevalier H. A. 1981; Phospholipids in the taxonomy of actinomycetes. Zentralbl Bakteriol Hyg Abt 1:Suppl 11111–116
     [Google Scholar]
  30. Lee S. D., Hah Y. C. 2002; Proposal to transfer Catellatospora ferruginea and ‘Catellatospora ishikariense’ to Asanoa gen. nov. as Asanoa ferruginea comb. nov. and Asanoa ishikariensis sp. nov., with emended description of the genus Catellatospora . Int J Syst Evol Microbiol 52:967–972 [View Article][PubMed]
     [Google Scholar]
  31. Lee D. W., Lee S. D. 2011; Allocatelliglobosispora scoriae gen. nov., sp. nov., isolated from volcanic ash. Int J Syst Evol Microbiol 61:264–270 [View Article][PubMed]
     [Google Scholar]
  32. Maldonado L. A., Fenical W., Jensen P. R., Kauffman C. A., Mincer T. J., Ward A. C., Bull A. T., Goodfellow M. 2005; Salinispora arenicola gen. nov., sp. nov. and Salinispora tropica sp. nov., obligate marine actinomycetes belonging to the family Micromonosporaceae . Int J Syst Evol Microbiol 55:1759–1766 [View Article][PubMed]
     [Google Scholar]
  33. Matsumoto A., Takahashi Y., Shinose M., Seino A., Iwai Y., Omura S. 2003; Longispora albida gen. nov., sp. nov., a novel genus of the family Micromonosporaceae . Int J Syst Evol Microbiol 53:1553–1559 [View Article][PubMed]
     [Google Scholar]
  34. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
     [Google Scholar]
  35. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. K. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
     [Google Scholar]
  36. Monciardini P., Cavaletti L., Ranghetti A., Schumann P., Rohde M., Bamonte R., Sosio M., Mezzelani A., Donadio S. 2009; Novel members of the family Micromonosporaceae, Rugosimonospora acidiphila gen. nov., sp. nov. and Rugosimonospora africana sp. nov.. Int J Syst Evol Microbiol 59:2752–2758 [View Article][PubMed]
     [Google Scholar]
  37. Nakajima Y., Kitpreechavanich V., Suzuki K., Kudo T. 1999; Microbispora corallina sp. nov., a new species of the genus Microbispora isolated from Thai soil. Int J Syst Bacteriol 49:1761–1767 [View Article][PubMed]
     [Google Scholar]
  38. Oliver J. D., Colwell R. R. 1973; Extractable lipids of Gram-negative marine bacteria: fatty-acid composition. Int J Syst Bacteriol 23:442–458 [View Article]
     [Google Scholar]
  39. Ørskov, J. (1923). Investigations into the morphology of the ray fungi. Copenhagen: Levin and Munksgaard.
  40. Pospiech A., Neumann B. 1995; A versatile quick-prep of genomic DNA from Gram-positive bacteria. Trends Genet 11:217–218 [View Article][PubMed]
     [Google Scholar]
  41. Qin S., Li J., Zhang Y. Q., Zhu W. Y., Zhao G. Z., Xu L. H., Li W. J. 2009; Plantactinospora mayteni gen. nov., sp. nov., a member of the family Micromonosporaceae . Int J Syst Evol Microbiol 59:2527–2533 [View Article][PubMed]
     [Google Scholar]
  42. Raper K. B., Fennell D. I. 1965 The Genus Aspergillus Baltimore, MD: Williams and Wilkins Company;
     [Google Scholar]
  43. Rheims H., Schumann P., Rohde M., Stackebrandt E. 1998; Verrucosispora gifhornensis gen. nov., sp. nov., a new member of the actinobacterial family Micromonosporaceae . Int J Syst Bacteriol 48:1119–1127 [View Article][PubMed]
     [Google Scholar]
  44. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
     [Google Scholar]
  45. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI technical note 101. Newark, DE: MIDI Inc.
  46. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [View Article]
     [Google Scholar]
  47. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov.. Int J Syst Bacteriol 47:479–491 [View Article]
     [Google Scholar]
  48. Tamura T., Nakagaito Y., Nishii T., Hasegawa T., Stackebrandt E., Yokota A. 1994; A new genus of the order Actinomycetales, Couchioplanes gen. nov., with descriptions of Couchioplanes caeruleus (Horan and Brodsky 1986) comb. nov. and Couchioplanes caeruleus subsp. azureus subsp. nov.. Int J Syst Bacteriol 44:193–203 [View Article][PubMed]
     [Google Scholar]
  49. Tamura T., Hayakawa M., Hatano K. 1997; A new genus of the order Actinomycetales, Spirilliplanes gen. nov., with description of Spirilliplanes yamanashiensis sp. nov.. Int J Syst Bacteriol 47:97–102 [View Article][PubMed]
     [Google Scholar]
  50. Tamura T., Hayakawa M., Hatano K. 2001; A new genus of the order Actinomycetales, Virgosporangium gen. nov., with descriptions of Virgosporangium ochraceum sp. nov. and Virgosporangium aurantiacum sp. nov.. Int J Syst Evol Microbiol 51:1809–1816[PubMed] [CrossRef]
     [Google Scholar]
  51. Tamura T., Hatano K., Suzuki K. 2006; A new genus of the family Micromonosporaceae, Polymorphospora gen. nov., with description of Polymorphospora rubra sp. nov.. Int J Syst Evol Microbiol 56:1959–1964 [View Article][PubMed]
     [Google Scholar]
  52. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
     [Google Scholar]
  53. Thawai C., Tanasupawat S., Itoh T., Kudo T. 2006; Actinocatenispora thailandica gen. nov., sp. nov., a new member of the family Micromonosporaceae . Int J Syst Evol Microbiol 56:1789–1794 [View Article][PubMed]
     [Google Scholar]
  54. Thawai C., Tanasupawat S., Kudo T. 2008; Micromonospora pattaloongensis sp. nov., isolated from a Thai mangrove forest. Int J Syst Evol Microbiol 58:1516–1521 [View Article][PubMed]
     [Google Scholar]
  55. Thawai C., Tanasupawat S., Suwanborirux K., Kudo T. 2010; Actinaurispora siamensis gen. nov., sp. nov., a new member of the family Micromonosporaceae . Int J Syst Evol Microbiol 60:1660–1666 [View Article][PubMed]
     [Google Scholar]
  56. Thiemann J. E., Pagani H., Beretta G. 1967; A new genus of the Actinoplanaceae: Dactylosporangium, gen. nov.. Arch Mikrobiol 58:42–52 [View Article][PubMed]
     [Google Scholar]
  57. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
     [Google Scholar]
  58. Uchida K., Aida A. 1977; Acyl type of bacterial cell wall: its simple identification by colorimetric method. J Gen Appl Microbiol 23:249–260 [View Article]
     [Google Scholar]
  59. Wassill L., Ludwig W., Schleifer K. H. 1998; Development of a modified subtraction hybridization technique and its application for the design of strain specific PCR systems for lactococci. FEMS Microbiol Lett 166:63–70 [View Article]
     [Google Scholar]
  60. Wiese J., Jiang Y., Tang S. K., Thiel V., Schmaljohann R., Xu L. H., Jiang C. L., Imhoff J. F. 2008; A new member of the family Micromonosporaceae, Planosporangium flavigriseum gen. nov., sp. nov.. Int J Syst Evol Microbiol 58:1324–1331 [View Article][PubMed]
     [Google Scholar]
  61. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J. 1983; Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813[PubMed]
     [Google Scholar]
  62. Williams S. T., Lanning S., Wellington E. M. H. 1984; Ecology of actinomycetes. In The Biology of the Actinomycetes pp. 481–528 Edited by Goodfellow M., Mordarski M., Williams S. T. London, UK: Academic Press;
     [Google Scholar]
  63. Yokota A., Tamura T., Hasegawa T., Huang L. H. 1993; Catenuloplanes japonicas gen. nov., sp. nov., nom. rev., a new genus of the order Actinomycetales . Int J Syst Bacteriol 43:805–812 [View Article]
     [Google Scholar]
  64. Zhi X. Y., Li W. J., Stackebrandt E. 2009; An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. Int J Syst Evol Microbiol 59:589–608 [View Article][PubMed]
     [Google Scholar]
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Jishengella endophytica gen. nov., sp. nov., a new member of the family Micromonosporaceae
Int J Syst Evol Microbiol 61, 1153 (2011); https://doi.org/10.1099/ijs.0.025288-0
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