%0 Journal Article %A Hoover, Richard B. %A Pikuta, Elena V. %A Bej, Asim K. %A Marsic, Damien %A Whitman, William B. %A Tang, Jane %A Krader, Paul %T Spirochaeta americana sp. nov., a new haloalkaliphilic, obligately anaerobic spirochaete isolated from soda Mono Lake in California %D 2003 %J International Journal of Systematic and Evolutionary Microbiology, %V 53 %N 3 %P 815-821 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.02535-0 %I Microbiology Society, %X A novel, obligately anaerobic, mesophilic, haloalkaliphilic spirochaete, strain ASpG1T, was isolated from sediments of the alkaline, hypersaline Mono Lake in California, USA. Cells of the Gram-negative strain were motile and spirochaete-shaped with sizes of 0·2–0·22×8–18 μm. Growth of the strain was observed between 10 and 44 °C (optimum 37 °C), in 2–12 % (w/v) NaCl (optimum 3 % NaCl) and between pH 8 and 10·5 (optimum pH 9·5). The novel strain was strictly alkaliphilic, required high concentrations of carbonates in the medium and was capable of utilizing d-glucose, fructose, maltose, sucrose, starch and d-mannitol. End products of glucose fermentation were H2, acetate, ethanol and formate. Strain ASpG1T was resistant to kanamycin and rifampicin, but sensitive to gentamicin, tetracycline and chloramphenicol. The G+C content of its DNA was 58·5 mol%. DNA–DNA hybridization analysis of strain ASpG1T with its most closely related species, Spirochaeta alkalica Z-7491T, revealed a hybridization value of only 48·7 %. On the basis of its physiological and molecular properties, strain ASpG1T appears to represent a novel species of the genus Spirochaeta, for which the name Spirochaeta americana is proposed (type strain ASpG1T=ATCC BAA-392T=DSM 14872T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.02535-0