1887

Abstract

A novel non-sporulating, non-motile, catalase- and oxidase-positive, strictly aerobic, Gram-negative, rod-shaped bacterial strain, designated DCA-1, was isolated from activated sludge collected from a butachlor wastewater treatment facility. The strain was able to degrade about 85 % of 100 mg butachlor l within 5 days of incubation. Growth occurred in the presence of 0–6 % (w/v) NaCl [optimum, 1 % (w/v) NaCl] and at pH 5.5–9.0 (optimum, pH 7.0) and 15–35 °C (optimum, 25–30 °C). Vesicular internal membrane structures and photoheterotrophic growth were not observed. The major respiratory quinone was ubiquinone 10 (Q-10) and the major cellular fatty acids were Cω7 and 11-methyl Cω7. The genomic DNA G+C content of strain DCA-1 was 62.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that strain DCA-1 was a member of the family and was related most closely to the type strain of (96.5 % sequence similarity). The combination of phylogenetic analysis, phenotypic characteristics and chemotaxonomic data supports the suggestion that strain DCA-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DCA-1 ( = CGMCC 1.7745  = DSM 21823). In addition, based on the characterization data obtained in this study, it is proposed that should be reclassified as comb. nov. (type strain JA139  = DSM 18774  = CCUG 53722  = JCM 14338). An emended description of the genus is also presented.

Funding
This study was supported by the:
  • National High Technology Research and Development Program of China (Award 2006AA10Z402)
  • National Basic Research Program of China (Award 2010CB833800)
  • Natural Science Foundation of Jiangsu Province, China (Award BK2008331)
  • Chinese National Technology Platform Programs (Award 2005DKA21201-2)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.025494-0
2011-08-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/8/1921.html?itemId=/content/journal/ijsem/10.1099/ijs.0.025494-0&mimeType=html&fmt=ahah

References

  1. Anil Kumar P., Srinivas T. N. R., Sasikala Ch., Ramana Ch. V. 2007; Rhodobacter changlensis sp. nov., a psychrotolerant, phototrophic alphaproteobacterium from the Himalayas of India. Int J Syst Evol Microbiol 57:2568–2571 [View Article][PubMed]
    [Google Scholar]
  2. Atlas R. M., Parks L. C. 1993 Handbook of Microbiological Media Edited by Parks L. C. Boca Raton, FL: CRC Press;
    [Google Scholar]
  3. Chakraborty S. K., Bhattacharyya A. 1991; Degradation of butachlor by two soil fungi. Chemosphere 23:99–105 [View Article]
    [Google Scholar]
  4. Chen Y.-G., Cui X.-L., Pukall R., Li H.-M., Yang Y.-L., Xu L.-H., Wen M.-L., Peng Q., Jiang C.-L. 2007; Salinicoccus kunmingensis sp. nov., a moderately halophilic bacterium isolated from a salt mine in Yunnan, south-west China. Int J Syst Evol Microbiol 57:2327–2332 [View Article][PubMed]
    [Google Scholar]
  5. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  6. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  7. Cui X.-L., Mao P.-H., Zeng M., Li W.-J., Zhang L.-P., Xu L.-H., Jiang C.-L. 2001; Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae . Int J Syst Evol Microbiol 51:357–363[PubMed]
    [Google Scholar]
  8. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  9. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  10. Felsenstein, J. (2002). phylip (phylogeny inference package), version 3.6a. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  11. Gregersen T. 1978; Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5:123–127 [View Article]
    [Google Scholar]
  12. Hanada S., Takaichi S., Matsuura K., Nakamura K. 2002; Roseiflexus castenholzii gen. nov., sp. nov., a thermophilic, filamentous, photosynthetic bacterium that lacks chlorosomes. Int J Syst Evol Microbiol 52:187–193[PubMed] [CrossRef]
    [Google Scholar]
  13. Hopwood D. A., Bibb M. J., Chater K. F., Kieser T., Bruton C. J., Kieser H. M., Lydiate D. J., Smith C. P., Ward J. M. 1985 Genetic Manipulation of Streptomyces: a Laboratory Manual Norwich: John Innes Foundation;
    [Google Scholar]
  14. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  15. Kluge A. G., Farris F. S. 1969; Quantitative phyletics and the evolution of anurans. Syst Zool 18:1–32 [View Article]
    [Google Scholar]
  16. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67 [View Article]
    [Google Scholar]
  17. Liu Y., Xu C.-J., Jiang J.-T., Liu Y.-H., Song X.-F., Li H., Liu Z.-P. 2010; Catellibacterium aquatile sp. nov., isolated from freshwater and emended description of the genus Catellibacterium Tanaka et al. 2004. Int J Syst Evol Microbiol 60:2027–2031 [View Article]
    [Google Scholar]
  18. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B:195–206 [View Article]
    [Google Scholar]
  19. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  20. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. Newark, DE: MIDI, Inc;
  21. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  22. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
    [Google Scholar]
  23. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetic analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  24. Tanaka Y., Hanada S., Manome A., Tsuchida T., Kurane R., Nakamura K., Kamagata Y. 2004; Catellibacterium nectariphilum gen. nov., sp. nov., which requires a diffusible compound from a strain related to the genus Sphingomonas for vigorous growth. Int J Syst Evol Microbiol 54:955–959 [View Article][PubMed]
    [Google Scholar]
  25. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  26. Yoon J.-H., Yeo S.-H., Oh T.-K. 2004; Hongiella marincola sp. nov., isolated from sea water of the East Sea in Korea. Int J Syst Evol Microbiol 54:1845–1848 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.025494-0
Loading
/content/journal/ijsem/10.1099/ijs.0.025494-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

PDF

Supplementary material 3

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error