1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 62, Issue 1

gen. nov., sp. nov., isolated from coastal seawater Free

Abstract

A novel Gram-negative, non-motile bacterium, designated ZXM137, was isolated from seawater collected from a coastal region of Qingdao, China, during a massive green algae () bloom. Strain ZXM137 was strictly aerobic and did not accumulate poly-β-hydroxybutyrate. Growth occurred with 0.5–11.0 % (w/v) NaCl, at pH 6–9 (optimum of pH 7) and at 4–45 °C (optimum at 28 °C). It contained Q-10 as the predominant ubiquinone and the major polar lipids were phosphatidylglycerol, phospholipids, and an unidentified aminolipid and lipid. The major cellular fatty acids of strain ZXM137 were Cω7, Cω6 and 11-methyl Cω7. The DNA G+C content was 60.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain ZXM137 formed a distinct evolutionary lineage within the group in the class . On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, strain ZXM137 represents a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed; the type strain is ZXM137 ( = CGMCC 1.8891 = LMG 24854).

  • Published Online:
Funding
This study was supported by the:
  • National High Technology R&D Program of China (Award 2007AA09Z434)
  • National Natural Science Foundation of China (Award 40876067)
© 2012 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.025536-0
2012-01-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/1/223.html?itemId=/content/journal/ijsem/10.1099/ijs.0.025536-0&mimeType=html&fmt=ahah

References

  1. Adachi M., Kanno T., Okamoto R., Shinozaki A., Fujikawa-Adachi K., Nishijima T. 2004; Jannaschia cystaugens sp. nov., an Alexandrium (Dinophyceae) cyst formation-promoting bacterium from Hiroshima Bay, Japan. Int J Syst Evol Microbiol 54:1687–1692 [View Article][PubMed]
     [Google Scholar]
  2. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. 1995 Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology, 3rd edn. New York: Wiley;
     [Google Scholar]
  3. Biebl H., Allgaier M., Lünsdorf H., Pukall R., Tindall B. J., Wagner-Döbler I. 2005; Roseovarius mucosus sp. nov., a member of the Roseobacter clade with trace amounts of bacteriochlorophyll a . Int J Syst Evol Microbiol 55:2377–2383 [View Article][PubMed]
     [Google Scholar]
  4. Boettcher K. J., Geaghan K. K., Maloy A. P., Barber B. J. 2005; Roseovarius crassostreae sp. nov., a member of the Roseobacter clade and the apparent cause of juvenile oyster disease (JOD) in cultured Eastern oysters. Int J Syst Evol Microbiol 55:1531–1537 [View Article][PubMed]
     [Google Scholar]
  5. Breznak J. A., Costilow R. N. 1994; Physicochemical factors in growth. In Methods for General and Molecular Bacteriology pp. 137–154 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  6. Buchan A., González J. M., Moran M. A. 2005; Overview of the marine roseobacter lineage. Appl Environ Microbiol 71:5665–5677 [View Article][PubMed]
     [Google Scholar]
  7. Cho J.-C., Giovannoni S. J. 2004; Oceanicola granulosus gen. nov., sp. nov. and Oceanicola batsensis sp. nov., poly-β-hydroxybutyrate-producing marine bacteria in the order ‘Rhodobacterales’. Int J Syst Evol Microbiol 54:1129–1136 [View Article][PubMed]
     [Google Scholar]
  8. Choi D. H., Yi H., Chun J., Cho B. C. 2006; Jannaschia seosinensis sp. nov., isolated from hypersaline water of a solar saltern in Korea. Int J Syst Evol Microbiol 56:45–49 [View Article][PubMed]
     [Google Scholar]
  9. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
     [Google Scholar]
  10. Felsenstein J. 2009; phylip (phylogeny inference package) v3.69. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  11. González J. M., Simó R., Massana R., Covert J. S., Casamayor E. O., Pedrós-Alió C., Moran M. A. 2000; Bacterial community structure associated with a dimethylsulfoniopropionate-producing North Atlantic algal bloom. Appl Environ Microbiol 66:4237–4246 [View Article][PubMed]
     [Google Scholar]
  12. González J. M., Covert J. S., Whitman W. B., Henriksen J. R., Mayer F., Scharf B., Schmitt R., Buchan A., Fuhrman J. A. other authors 2003; Silicibacter pomeroyi sp. nov. and Roseovarius nubinhibens sp. nov., dimethylsulfoniopropionate-demethylating bacteria from marine environments. Int J Syst Evol Microbiol 53:1261–1269 [View Article][PubMed]
     [Google Scholar]
  13. Gu J., Guo B., Wang Y.-N., Yu S.-L., Inamori R., Qu R., Ye Y.-G., Wu X.-L. 2007; Oceanicola nanhaiensis sp. nov., isolated from sediments of the South China Sea. Int J Syst Evol Microbiol 57:157–160 [View Article][PubMed]
     [Google Scholar]
  14. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
     [Google Scholar]
  15. Hosoya S., Yokota A. 2007; Loktanella atrilutea sp. nov., isolated from seawater in Japan. Int J Syst Evol Microbiol 57:1966–1969 [View Article][PubMed]
     [Google Scholar]
  16. Ivanova E. P., Zhukova N. V., Lysenko A. M., Gorshkova N. M., Sergeev A. F., Mikhailov V. V., Bowman J. P. 2005; Loktanella agnita sp. nov. and Loktanella rosea sp. nov., from the north-west Pacific Ocean. Int J Syst Evol Microbiol 55:2203–2207 [View Article][PubMed]
     [Google Scholar]
  17. Ivanova E. P., Webb H., Christen R., Zhukova N. V., Kurilenko V. V., Kalinovskaya N. I., Crawford R. J. 2010; Celeribacter neptunius gen. nov., sp. nov., a new member of the class Alphaproteobacteria. . Int J Syst Evol Microbiol 60:1620–1625 [CrossRef]
     [Google Scholar]
  18. Kim B. Y., Yoo S. H., Weon H. Y., Jeon Y. A., Hong S. B., Go S. J., Stackebrandt E., Kwon S. W. 2008; Jannaschia pohangensis sp. nov., isolated from seashore sand in Korea. Int J Syst Evol Microbiol 58:496–499 [View Article][PubMed]
     [Google Scholar]
  19. Kim B. Y., Weon H. Y., Son J. A., Lee C. M., Hong S. B., Jeon Y. A., Koo B. S., Kwon S. W. 2009; Thalassobacter arenae sp. nov., isolated from sea sand in Korea. Int J Syst Evol Microbiol 59:487–490 [View Article][PubMed]
     [Google Scholar]
  20. Labrenz M., Collins M. D., Lawson P. A., Tindall B. J., Schumann P., Hirsch P. 1999; Roseovarius tolerans gen. nov., sp. nov., a budding bacterium with variable bacteriochlorophyll a production from hypersaline Ekho Lake. Int J Syst Bacteriol 49:137–147 [View Article][PubMed]
     [Google Scholar]
  21. Labrenz M., Lawson P. A., Tindall B. J., Collins M. D., Hirsch P. 2005; Roseisalinus antarcticus gen. nov., sp. nov., a novel aerobic bacteriochlorophyll a-producing α-proteobacterium isolated from hypersaline Ekho Lake, Antarctica. Int J Syst Evol Microbiol 55:41–47 [View Article][PubMed]
     [Google Scholar]
  22. Lau S. C. K., Tsoi M. M. Y., Li X., Plakhotnikova I., Wu M., Wong P.-K., Qian P.-Y. 2004; Loktanella hongkongensis sp. nov., a novel member of the α-Proteobacteria originating from marine biofilms in Hong Kong waters. Int J Syst Evol Microbiol 54:2281–2284 [View Article][PubMed]
     [Google Scholar]
  23. Lin K. Y., Sheu S. Y., Chang P. S., Cho J. C., Chen W. M. 2007; Oceanicola marinus sp. nov., a marine alphaproteobacterium isolated from seawater collected off Taiwan. Int J Syst Evol Microbiol 57:1625–1629 [View Article][PubMed]
     [Google Scholar]
  24. Lyman J., Fleming R. H. 1940; Composition of seawater. J Mar Res 3:134–146
     [Google Scholar]
  25. Macián M. C., Arahal D. R., Garay E., Ludwig W., Schleifer K. H., Pujalte M. J. 2005a; Thalassobacter stenotrophicus gen. nov., sp. nov., a novel marine α-proteobacterium isolated from Mediterranean sea water. Int J Syst Evol Microbiol 55:105–110 [View Article][PubMed]
     [Google Scholar]
  26. Macián M. C., Arahal D. R., Garay E., Ludwig W., Schleifer K. H., Pujalte M. J. 2005b; Jannaschia rubra sp. nov., a red-pigmented bacterium isolated from sea water. Int J Syst Evol Microbiol 55:649–653 [View Article][PubMed]
     [Google Scholar]
  27. Pujalte M. J., Macián M. C., Arahal D. R., Garay E. 2005; Thalassobacter stenotrophicus Macian et al. 2005 is a later synonym of Jannaschia cystaugens Adachi et al. 2004, with emended description of the genus Thalassobacter . Int J Syst Evol Microbiol 55:1959–1963 [View Article][PubMed]
     [Google Scholar]
  28. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. Technical Note 101. Newark, DE: MIDI;
  29. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
     [Google Scholar]
  30. Tamura K., Dudley J., Nei M., Kumar S. 2007; MEGA4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
     [Google Scholar]
  31. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
     [Google Scholar]
  32. Tindall B. J., Sikorski J., Smibert R. M., Krieg N. R. 2007; Phenotypic characterization and the principles of comparative systematics. In Methods for General and Molecular Microbiology pp. 330–393 Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  33. Van Trappen S., Mergaert J., Swings J. 2004; Loktanella salsilacus gen. nov., sp. nov., Loktanella fryxellensis sp. nov. and Loktanella vestfoldensis sp. nov., new members of the Rhodobacter group, isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 54:1263–1269 [View Article][PubMed]
     [Google Scholar]
  34. Wagner-Döbler I., Rheims H., Felske A., Pukall R., Tindall B. J. 2003; Jannaschia helgolandensis gen. nov., sp. nov., a novel abundant member of the marine Roseobacter clade from the North Sea. Int J Syst Evol Microbiol 53:731–738 [View Article][PubMed]
     [Google Scholar]
  35. Wang B. J., Tan T. F., Shao Z. Z. 2009; Roseovarius pacificus sp. nov., isolated from deep-sea sediment. Int J Syst Evol Microbiol 59:1116–1121 [View Article][PubMed]
     [Google Scholar]
  36. Weon H. Y., Kim B. Y., Yoo S. H., Kim J. S., Kwon S. W., Go S. J., Stackebrandt E. 2006; Loktanella koreensis sp. nov., isolated from sea sand in Korea. Int J Syst Evol Microbiol 56:2199–2202 [View Article][PubMed]
     [Google Scholar]
  37. Yoon J. H., Kang S. J., Park S., Oh T. K. 2007a; Jannaschia donghaensis sp. nov., isolated from seawater of the East Sea, Korea. Int J Syst Evol Microbiol 57:2132–2136 [View Article][PubMed]
     [Google Scholar]
  38. Yoon J.-H., Kang S.-J., Lee S.-Y., Oh T.-K. 2007b; Loktanella maricola sp. nov., isolated from seawater of the East Sea in Korea. Int J Syst Evol Microbiol 57:1799–1802 [View Article][PubMed]
     [Google Scholar]
  39. Yoon J. H., Kang S. J., Oh T. K. 2008; Roseovarius aestuarii sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 58:1198–1202 [View Article][PubMed]
     [Google Scholar]
  40. Yuan J., Lai Q.-L., Wang B.-J., Sun F.-Q., Liu X., Du Y., Li G., Gu L., Zheng T., Shao Z. 2009; Oceanicola pacificus sp. nov., isolated from a deep-sea pyrene-degrading consortium. Int J Syst Evol Microbiol 59:1158–1161 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.025536-0
Loading
Huaishuia halophila gen. nov., sp. nov., isolated from coastal seawater
Int J Syst Evol Microbiol 62, 223 (2012); https://doi.org/10.1099/ijs.0.025536-0
/content/journal/ijsem/10.1099/ijs.0.025536-0
/content/journal/ijsem/10.1099/ijs.0.025536-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error