RT Journal Article SR Electronic(1) A1 Grégoire, Patrick A1 Bohli, Malek A1 Cayol, Jean-Luc A1 Joseph, Manon A1 Guasco, Sophie A1 Dubourg, Karine A1 Cambar, Jean A1 Michotey, Valérie A1 Bonin, Patricia A1 Fardeau, Marie-Laure A1 Ollivier, BernardYR 2011 T1 Caldilinea tarbellica sp. nov., a filamentous, thermophilic, anaerobic bacterium isolated from a deep hot aquifer in the Aquitaine Basin JF International Journal of Systematic and Evolutionary Microbiology, VO 61 IS 6 SP 1436 OP 1441 DO https://doi.org/10.1099/ijs.0.025676-0 PB Microbiology Society, SN 1466-5034, AB An anaerobic, thermophilic, filamentous (0.45 × >100 µm) bacterium, designated D1-25-10-4T, was isolated from a deep hot aquifer in France. Cells were non-motile and Gram-negative. Growth was observed at 43–65 °C (optimum 55 °C), at pH 6.8–7.8 (optimum pH 7.0) and with 0–5 g NaCl l−1 (optimum 0 g NaCl l−1). Strain D1-25-10-4T was a chemo-organotroph and fermented ribose, maltose, glucose, galactose, arabinose, fructose, mannose, sucrose, raffinose, xylose, glycerol, fumarate, peptone, starch and xylan. Yeast extract was required for growth. Sulfate, thiosulfate, sulfite, elemental sulfur, nitrate, nitrite and fumarate were not used as terminal electron acceptors. The G+C content of the DNA was 61.9 mol%. The major cellular fatty acids of strain D1-25-10-4T were C17 : 0, C18 : 0, C16 : 0 and iso-C17 : 0. The closest phylogenetic relative of strain D1-25-10-4T was Caldilinea aerophila STL-6-O1T (97.9 % 16S rRNA gene sequence similarity). DNA–DNA relatedness between strain D1-25-10-4T and Caldilinea aerophila DSM 14535T was 8.7±1 %. On the basis of phylogenetic, genotypic and phenotypic characteristics, strain D1-25-10-4T represents a novel species within the genus Caldilinea, class Caldilineae, phylum Chloroflexi, for which the name Caldilinea tarbellica sp. nov. is proposed. The type strain is D1-25-10-4T ( = DSM 22659T  = JCM 16120T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.025676-0