1887

Abstract

A Gram-stain-negative, aerobic, rod-shaped bacterium, designated strain J3-A127, was isolated from the roots of fresh rice plants (). Cells were non-motile and no flagellum was detected. Comparison of 16S rRNA gene sequences indicated that the strain was phylogenetically related to species of the genus , with closest similarity to Alt 505 (96.4 %). The low levels of 16S rRNA gene sequence similarity (<90 %) found between the , , and gene sequences of strain J3-A127 and the type strains of recognized species of the genus also indicated that it represented a separate species. The temperature range for growth was 10–40 °C (optimum around 28 °C) and the pH range was 6.0–11.0 (optimum pH 7.0–8.0). Strain J3-A127 tolerated NaCl concentrations up to 5.0 % (w/v). The strain was catalase- and oxidase-positive. The main cellular fatty acids were summed feature 8 (Cω7 and/or Cω6; 46.7 %). The DNA G+C content of strain J3-A127 was 59.5 mol%. Strain J3-A127 did not form any nodules on four different legumes and the and genes were not detected by PCR. According to physiological and biochemical characteristics and genotypic data, strain J3-A127 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is J3-A127 ( = ACCC 10380 = KCTC 23294).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 30900001)
  • Special scientific research funds for commonwealth agricultural industry (Award 200803029)
  • special foundation for central academy of the Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences (Award 2009-10)
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2011-10-01
2024-04-23
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