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Volume 61, Issue 9

sp. nov., a slightly halophilic bacterium isolated from non-saline forest soil Free

Abstract

A novel Gram-stain-positive, slightly halophilic, catalase-positive, oxidase-negative, endospore-forming, motile, facultatively anaerobic, rod-shaped bacterium, designated strain JSM 081004, was isolated from non-saline forest soil in Xiaoxi National Natural Reserve, China. Growth occurred with 0.5–20 % (w/v) NaCl (optimum 2–4 %), at pH 6.0–10.5 (optimum pH 8.0) and at 5–40 °C (optimum 25–30 °C). -Diaminopimelic acid was present in the cell-wall peptidoglycan. The major cellular fatty acids were iso-C and anteiso-C. Strain JSM 081004 contained MK-7 as the predominant respiratory quinone, and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol as the major polar lipids. The genomic DNA G+C content of strain JSM 081004 was 40.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain JSM 081004 should be assigned to the genus and was most closely related to the type strains of (sequence similarity 97.8 %), (97.8 %) and (97.3 %). Phylogenetic analysis, DNA–DNA relatedness values, phenotypic characteristics and chemotaxonomic data all support the proposal of strain JSM 081004 as a representative of a novel species of the genus , for which the name sp. nov. is proposed; the type strain is JSM 081004 ( = CCTCC AA 208057  = DSM 21943).

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Funding
This study was supported by the:
  • National Basic Research Program of China (Award 2010CB833800)
  • National Natural Science Foundation of China (NSFC) (Award 30970007 and 30860013)
  • Key Laboratory of Ecotourism’s Application Technology of Hunan Province (Award SL2010Z01)
  • International Cooperation Research Program of Yunnan Province (Award 2009AC017)
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2011-09-01
2024-04-25
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References

  1. Arahal D. R., Ventosa A. 2002; Moderately halophilic and halotolerant species of Bacillus and related genera. In Applications and Systematics of Bacillus and Relatives pp. 83–99 Edited by Berkeley R. C. W., Heyndrickx M., Logan N., De Vos P. Oxford: Blackwell; [View Article]
     [Google Scholar]
  2. Ash C., Farrow J. A. E., Wallbanks S., Collins M. D. 1991; Phylogenetic heterogeneity of the genus Bacillus as revealed by comparative analysis of small-subunit-ribosomal RNA sequences. Lett Appl Microbiol 13:202–206 [View Article]
     [Google Scholar]
  3. Atlas R. M. 1993 Handbook of Microbiological Media Edited by Parks L. C. Boca Raton, FL: CRC Press;
     [Google Scholar]
  4. Chen Y.-G., Cui X.-L., Pukall R., Li H.-M., Yang Y.-L., Xu L.-H., Wen M.-L., Peng Q., Jiang C.-L. 2007; Salinicoccus kunmingensis sp. nov., a moderately halophilic bacterium isolated from a salt mine in Yunnan, south-west China. Int J Syst Evol Microbiol 57:2327–2332 [View Article][PubMed]
     [Google Scholar]
  5. Chen Y.-G., Zhang Y.-Q., Xiao H.-D., Liu Z.-X., Yi L.-B., Shi J.-X., Zhi X.-Y., Cui X.-L., Li W.-J. 2009a; Pontibacillus halophilus sp. nov., a moderately halophilic bacterium isolated from a sea urchin. Int J Syst Evol Microbiol 59:1635–1639 [View Article][PubMed]
     [Google Scholar]
  6. Chen Y.-G., Zhang Y.-Q., Wang Y.-X., Liu Z.-X., Klenk H.-P., Xiao H.-D., Tang S.-K., Cui X.-L., Li W.-J. 2009b; Bacillus neizhouensis sp. nov., a halophilic marine bacterium isolated from a sea anemone. Int J Syst Evol Microbiol 59:3035–3039 [View Article][PubMed]
     [Google Scholar]
  7. Chen Q., Liu Z., Peng Q., Huang K., He J., Zhang L., Li W., Chen Y. 2010; [Diversity of halophilic and halotolerant bacteria isolated from non-saline soil collected from Xiaoxi National Natural Reserve, Hunan Province]. Wei Sheng Wu Xue Bao 50:1452–1459 (in Chinese) [PubMed]
     [Google Scholar]
  8. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y.-W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
     [Google Scholar]
  9. Collins M. D., Jones D. 1980; Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48:459–470 [CrossRef]
     [Google Scholar]
  10. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
     [Google Scholar]
  11. Cui X.-L., Mao P.-H., Zeng M., Li W.-J., Zhang L.-P., Xu L.-H., Jiang C.-L. 2001; Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae . Int J Syst Evol Microbiol 51:357–363[PubMed]
     [Google Scholar]
  12. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [View Article][PubMed]
     [Google Scholar]
  13. Dittmer J. C., Lester R. L. 1964; A simple, specific spray for the detection of phospholipids on thin-layer chromatograms. J Lipid Res 15:126–127[PubMed]
     [Google Scholar]
  14. Echigo A., Hino M., Fukushima T., Mizuki T., Kamekura M., Usami R. 2005; Endospores of halophilic bacteria of the family Bacillaceae isolated from non-saline Japanese soil may be transported by Kosa event (Asian dust storm). Saline Syst 1:8 [View Article][PubMed]
     [Google Scholar]
  15. Echigo A., Fukushima T., Mizuki T., Kamekura M., Usami R. 2007; Halalkalibacillus halophilus gen. nov., sp. nov., a novel moderately halophilic and alkaliphilic bacterium isolated from a non-saline soil sample in Japan. Int J Syst Evol Microbiol 57:1081–1085 [View Article][PubMed]
     [Google Scholar]
  16. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
     [Google Scholar]
  17. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
     [Google Scholar]
  18. Felsenstein J. 2002; phylip (phylogeny inference package), version 3.6a. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA
  19. Ghosh A., Bhardwaj M., Satyanarayana T., Khurana M., Mayilraj S., Jain R. K. 2007; Bacillus lehensis sp. nov., an alkalitolerant bacterium isolated from soil. Int J Syst Evol Microbiol 57:238–242 [View Article][PubMed]
     [Google Scholar]
  20. Gregersen T. 1978; Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5:123–127 [View Article]
     [Google Scholar]
  21. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. 1996; Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239 [View Article][PubMed]
     [Google Scholar]
  22. Hasegawa T., Takizawa M., Tanida S. 1983; A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322 [View Article]
     [Google Scholar]
  23. Hopwood D. A., Bibb M. J., Chater K. F., Kieser T., Bruton C. J., Kieser H. M., Lydiate D. J., Smith C. P., Ward J. M., Schrempf H. (editors) 1985 Genetic Manipulation of Streptomyces. A Laboratory Manual Norwich: John Innes Foundation;
     [Google Scholar]
  24. Horikoshi K. 1999; Alkaliphiles: some applications of their products for biotechnology. Microbiol Mol Biol Rev 63:735–750[PubMed]
     [Google Scholar]
  25. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
     [Google Scholar]
  26. Jahnke K. D. 1992; basic computer program for evaluation of spectroscopic DNA renaturation data from Gilford System 2600 spectrophotometer on a PC/XT/AT type personal computer. J Microbiol Methods 15:61–73 [View Article]
     [Google Scholar]
  27. Kämpfer P. 1994; Limits and possibilities of total fatty acid analysis for classification and identification of Bacillus species. Syst Appl Microbiol 17:86–98 [CrossRef]
     [Google Scholar]
  28. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
     [Google Scholar]
  29. Kluge A. G., Farris F. S. 1969; Quantitative phyletics and the evolution of anurans. Syst Zool 18:1–32 [View Article]
     [Google Scholar]
  30. Krulwich T. A., Hicks D. B., Swartz T. H., Ito M. 2007; Bioenergetic adaptations that support alkaliphily. In Physiology and Biochemistry of Extremophiles pp. 311–329 Edited by Gerday C., Glansdorff N. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  31. Lim J.-M., Jeon C. O., Kim C.-J. 2006a; Bacillus taeanensis sp. nov., a halophilic Gram-positive bacterium from a solar saltern in Korea. Int J Syst Evol Microbiol 56:2903–2908 [View Article][PubMed]
     [Google Scholar]
  32. Lim J.-M., Jeon C. O., Lee S.-M., Lee J. C., Xu L. H., Jiang C. L., Kim C. J. 2006b; Bacillus salarius sp. nov., a halophilic, spore-forming bacterium isolated from a salt lake in China. Int J Syst Evol Microbiol 56:373–377 [View Article][PubMed]
     [Google Scholar]
  33. Margesin R., Schinner F. 2001; Potential of halotolerant and halophilic microorganisms for biotechnology. Extremophiles 5:73–83 [View Article][PubMed]
     [Google Scholar]
  34. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
     [Google Scholar]
  35. Minnikin D. E., Collins M. D., Goodfellow M. 1979; Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Microbiol 47:87–95
     [Google Scholar]
  36. Nielsen P., Rainey F. A., Outtrup H., Priest F. G., Fritze D. 1994; Comparative 16S rDNA sequence analysis of some alkaliphilic bacilli and the establishment of a sixth rRNA group within the genus Bacillus . FEMS Microbiol Lett 117:61–66 [View Article]
     [Google Scholar]
  37. Nielsen P., Fritze D., Priest F. G. 1995; Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species. Microbiology 141:1745–1761 [View Article]
     [Google Scholar]
  38. Nogi Y., Takami H., Horikoshi K. 2005; Characterization of alkaliphilic Bacillus strains used in industry: proposal of five novel species. Int J Syst Evol Microbiol 55:2309–2315 [View Article][PubMed]
     [Google Scholar]
  39. Olivera N., Siñeriz F., Breccia J. D. 2005; Bacillus patagoniensis sp. nov., a novel alkalitolerant bacterium from the rhizosphere of Atriplex lampa in Patagonia, Argentina. Int J Syst Evol Microbiol 55:443–447 [View Article][PubMed]
     [Google Scholar]
  40. Romano I., Lama L., Nicolaus B., Gambacorta A., Giordano A. 2005; Bacillus saliphilus sp. nov., isolated from a mineral pool in Campania, Italy. Int J Syst Evol Microbiol 55:159–163 [View Article][PubMed]
     [Google Scholar]
  41. Ryu E. K., MacCoss M. 1979; Modification of the Dittmer-Lester reagent for the detection of phospholipid derivatives on thin-layer chromatograms. J Lipid Res 20:561–563[PubMed]
     [Google Scholar]
  42. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
     [Google Scholar]
  43. Sasser M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  44. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  45. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
     [Google Scholar]
  46. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetic analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
     [Google Scholar]
  47. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
     [Google Scholar]
  48. Usami R., Echigo A., Fukushima T., Mizuki T., Yoshida Y., Kamekura M. 2007; Alkalibacillus silvisoli sp. nov., an alkaliphilic moderate halophile isolated from non-saline forest soil in Japan. Int J Syst Evol Microbiol 57:770–774 [View Article][PubMed]
     [Google Scholar]
  49. Vaskovsky V. E., Kostetsky E. Y., Vasendin I. M. 1975; A universal reagent for phospholipid analysis. J Chromatogr A 114:129–141 [View Article][PubMed]
     [Google Scholar]
  50. Ventosa A., Quesada E., Rodriguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A. 1982; Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128:1959–1968
     [Google Scholar]
  51. Ventosa A., Nieto J. J., Oren A. 1998; Biology of moderately halophilic aerobic bacteria. Microbiol Mol Biol Rev 62:504–544[PubMed]
     [Google Scholar]
  52. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
     [Google Scholar]
  53. Yumoto I. 2007; Environmental and taxonomic biodiversities of Gram-positive alkaliphiles. In Physiology and Biochemistry of Extremophiles pp. 295–310 Edited by Gerday C., Glansdorff N. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  54. Yumoto I., Hirota K., Goto T., Nodasaka Y., Nakajima K. 2005; Bacillus oshimensis sp. nov., a moderately halophilic, non-motile alkaliphile. Int J Syst Evol Microbiol 55:907–911 [View Article][PubMed]
     [Google Scholar]
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Bacillus xiaoxiensis sp. nov., a slightly halophilic bacterium isolated from non-saline forest soil
Int J Syst Evol Microbiol 61, 2095 (2011); https://doi.org/10.1099/ijs.0.026286-0
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