1887

Abstract

Six strains of anaerobic, Gram-negative coccobacilli isolated from the root canals of patients with endodontic infections (five strains) and from a deep periodontal pocket (one strain) were subjected to a comprehensive range of phenotypic and genetic tests and were found to comprise a homogeneous group. Following 16S rRNA gene sequence analysis, they were found to be most closely related to , with 93 % sequence similarity between the two taxa. A novel species, sp. nov., is proposed. Biochemically, the species is largely unreactive and is asaccharolytic, with only traces of acetate and propionate detected as metabolic end-products. The G+C content of the DNA of strains is 45–46 mol%. The type strain is E7.25 (=CCUG 47026=DSM 15470).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02640-0
2003-11-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/53/6/ijs531937.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02640-0&mimeType=html&fmt=ahah

References

  1. Bergey D. H., Harrison F. C., Breed R. S., Hammer B. W., Huntoon F. M. 1923 In Bergey's Manual of Determinative Bacteriology pp 271–272 Baltimore: Williams & Wilkins;
    [Google Scholar]
  2. Doan N., Contreras A., Flynn J., Slots J., Chen C. 2000; Molecular identification of Dialister pneumosintes in subgingival plaque of humans. J Clin Microbiol 38:3043–3047
    [Google Scholar]
  3. Downes J., Munson M. A., Radford D. R., Spratt D. A., Wade W. G. 2002; Shuttleworthia satelles gen. nov., sp. nov., isolated from the human oral cavity. Int J Syst Evol Microbiol 52:1469–1475 [CrossRef]
    [Google Scholar]
  4. Dymock D., Weightman A. J., Scully C., Wade W. G. 1996; Molecular analysis of microflora associated with dentoalveolar abscesses. J Clin Microbiol 34:537–542
    [Google Scholar]
  5. Felsenstein J. 1993 phylip (phylogeny inference package) version 3.5c. Distributed by the author Department of Genetics, University of Washington; Seattle, USA:
    [Google Scholar]
  6. Ghayoumi N., Chen C., Slots J. 2002; Dialister pneumosintes , a new putative periodontal pathogen. J Periodontal Res 37:75–78 [CrossRef]
    [Google Scholar]
  7. Goldstein E. J., Citron D. M., Finegold S. M. 1984; Role of anaerobic bacteria in bite-wound infections. Rev Infect Dis 6:S177–S183 [CrossRef]
    [Google Scholar]
  8. Holdeman L. V. H., Moore W. E. C. 1970 Outline of Clinical Methods in Anaerobic Microbiology Blacksburg, VA: Virginia Polytechnic Institute and State University;
    [Google Scholar]
  9. Holdeman L. V. H., Cato E. P., Moore W. E. C. 1977 Anaerobe Laboratory Manual , 4th edn. Blacksburg, VA: Virginia Polytechnic Institute and State University;
    [Google Scholar]
  10. Huß V. A. R., Festl H., Schleifer K.-H. 1983; Studies on the spectrophotometric determination of DNA hybridisation from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  11. Kroes I., Lepp P. W., Relman D. A. 1999; Bacterial diversity within the human subgingival crevice. Proc Natl Acad Sci U S A 96:14547–14552 [CrossRef]
    [Google Scholar]
  12. Maidak B. L., Cole J. R., Lilburn T. G. 7 other authors 2001; The RDP-II (Ribosomal Database Project. Nucleic Acids Res 29:173–174 [CrossRef]
    [Google Scholar]
  13. Milsom S. E. 1997 Applications of 16S rRNA analysis for the species and sub-species identification of oral bacteria MSc thesis University of; Bristol, UK:
    [Google Scholar]
  14. Milsom S. E., Dymock D., Wade W. G. 1996; Isolation and characterisation of Dialister pneumosintes . J Dent Research 75:1185
    [Google Scholar]
  15. Moore L. V. H., Moore W. E. C. 1994; Oribaculum catoniae gen. nov., sp. nov.; Catonella morbi gen. nov., sp. nov.; and Dialister pneumosintes gen. nov., comb. nov., nom. rev. anaerobic gram-negative bacilli from the human gingival crevice. Int J Syst Bacteriol 44187–192 [CrossRef]
    [Google Scholar]
  16. Munson M. A., Pitt Ford T., Chong B., Weightman A. J., Wade W. G. 2002; Molecular and cultural analysis of the microflora associated with endodontic infections. J Dent Research 81:761–766 [CrossRef]
    [Google Scholar]
  17. Olitsky P. K., Gates F. L. 1921; Experimental studies of the naso-pharyngeal secretions from influenza patients. J Exp Med 33:713–729 [CrossRef]
    [Google Scholar]
  18. Page R. D. M. 1996; TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358
    [Google Scholar]
  19. Paster B. J., Boches S. K., Galvin J. L., Ericson R. E., Lau C. N., Levanos V. A., Sahasrabudhe A., Dewhirst F. E. 2001; Bacterial diversity in human subgingival plaque. J Bacteriol 183:3770–3783 [CrossRef]
    [Google Scholar]
  20. Rousee J. M., Bermond D., Piemont Y., Tournoud C., Heller R., Kehrli P., Harlay M. L., Monteil H., Jaulhac B. 2002; Dialister pneumosintes associated with human brain abscesses. J Clin Microbiol 40:3871–3873 [CrossRef]
    [Google Scholar]
  21. Slayne M. A., Aldred M. J., Wade W. G. 1990; A rapid, semi-automated SDS-PAGE identification system for oral anaerobic bacteria. J Appl Bacteriol 68:391–395 [CrossRef]
    [Google Scholar]
  22. Summanen P., Baron E. J., Citron D. M., Strong C. A., Wexler H. M., Finegold S. M. 1993 Wadsworth Anaerobic Bacteriology Manual , 5th edn. Belmont, CA: Star Publishing;
    [Google Scholar]
  23. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  24. Wade W. G., Gray A. R., Absi E. G., Barker G. R. 1991; Predominant cultivable flora in pericoronitis. Oral Microbiol Immunol 6:310–312 [CrossRef]
    [Google Scholar]
  25. Wade W. G., Downes J., Dymock D., Hiom S. J., Weightman A. J., Dewhirst F. E., Paster B. J., Tzellas N., Coleman B. 1999; The family Coriobacteriaceae : reclassification of Eubacterium exiguum (Poco et al . 1996) and Peptostreptococcus heliotrinreducens (Lanigan 1976) as Slackia exigua gen. nov., comb. nov. and Slackia heliotrinireducens gen. nov., comb. nov., and Eubacterium lentum (Prevot 1938) as Eggerthella lenta gen. nov., comb. nov. Int J Syst Bacteriol 49595–600 [CrossRef]
    [Google Scholar]
  26. Willems A., Collins M. D. 1995; Phylogenetic placement of Dialister pneumosintes (formerly Bacteroides pneumosintes ) within the Sporomusa subbranch of the Clostridium subphylum of the gram-positive bacteria. Int J Syst Bacteriol 45:403–405 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02640-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02640-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error