1887

Abstract

Bacterial strain TM-1 was isolated from the gut of a wood-feeding termite, Snyder. Cells of strain TM-1 were Gram-negative, spherical (1.0–2.0 µm in diameter), non-motile, non-sporulating and red-pigmented. Strain TM-1 was resistant to UV radiation, showing 34 % survival after exposure to UV light at a dose of 100 J m. Growth occurred at 20–40 °C (optimum, 30 °C), at pH 6.0–10.0 (optimum, pH 6.0–7.0), and in the presence of 0–1 % (w/v) NaCl (optimum, 0–0.4 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain TM-1 was related to members of the genus , with sequence similarities ranging from 87.0 to 94.0 %. The peptidoglycan of strain TM-1 contained ornithine, alanine, glycine and glutamic acid. The most abundant cellular fatty acids of strain TM-1 were summed feature 3 (Cω7 and/or Cω6; 22.3 %) and C (37.5 %). MK-8 was the predominant quinone. The polar lipid profile contained three glycophospholipids, six glycolipids, one aminolipid and three unknown lipids. DNA of the type strain had a G+C content of 65.6 mol%. Based on the phylogenetic, chemotaxonomic and phenotypic data presented, strain TM-1 represents a novel species of the genus , for which the name sp. nov. is proposed, with TM-1 ( = CGMCC 1.10218 = NBRC 106334) as the type strain.

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 30970001 and 30570034)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.026567-0
2012-01-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/1/78.html?itemId=/content/journal/ijsem/10.1099/ijs.0.026567-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410[PubMed] [CrossRef]
    [Google Scholar]
  2. Anderson R., Hansen K. 1985; Structure of a novel phosphoglycolipid from Deinococcus radiodurans . J Biol Chem 260:12219–12223[PubMed]
    [Google Scholar]
  3. Asker D., Awad T. S., Beppu T., Ueda K. 2009; Deinococcus aquiradiocola sp. nov., isolated from a radioactive site in Japan. Int J Syst Evol Microbiol 59:144–149 [View Article][PubMed]
    [Google Scholar]
  4. Brooks B. W., Murray R. G. E. 1981; Nomenclature for “Micrococcus radiodurans” and other radiation-resistant cocci: Deinococcaceae fam. nov. and Deinococcus gen. nov., including five species. Int J Syst Bacteriol 31:353–360 [View Article]
    [Google Scholar]
  5. Brune A., Miambi E., Breznak J. A. 1995; Roles of oxygen and the intestinal microflora in the metabolism of lignin-derived phenylpropanoids and other monoaromatic compounds by termites. Appl Environ Microbiol 61:2688–2695[PubMed]
    [Google Scholar]
  6. Callegan R. P., Nobre M. F., McTernan P. M., Battista J. R., Navarro-González R., McKay C. P., da Costa M. S., Rainey F. A. 2008; Description of four novel psychrophilic, ionizing radiation-sensitive Deinococcus species from alpine environments. Int J Syst Evol Microbiol 58:1252–1258 [View Article][PubMed]
    [Google Scholar]
  7. Collins M. D. 1985; Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics pp. 267–287 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  8. Dittmer J. C., Lester R. L. 1964; A simple, specific spray for the detection of phospholipids on thin-layer chromatograms. J Lipid Res 15:126–127[PubMed]
    [Google Scholar]
  9. Dong X.-Z., Cai M.-Y. (editors) 2001; Determination of biochemical properties. In Manual for the Systematic Identification of General Bacteria pp. 370–398 Beijing: Scientific Press; (English translation)
    [Google Scholar]
  10. Eichler B., Pfennig N. 1986; Characterization of a new platelet-forming purple sulfur bacterium, Amoebobacter pedioformis sp. nov.. Arch Microbiol 146:295–300 [View Article]
    [Google Scholar]
  11. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  12. Felsenstein J. 1993 phylip (phylogeny inference package) version 3.5.1. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  13. Ferreira A. C., Nobre M. F., Rainey F. A., Silva M. T., Wait R., Burghardt J., Chung A. P., da Costa M. S. 1997; Deinococcus geothermalis sp. nov. and Deinococcus murrayi sp. nov., two extremely radiation-resistant and slightly thermophilic species from hot springs. Int J Syst Bacteriol 47:939–947 [View Article][PubMed]
    [Google Scholar]
  14. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. 1994 Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  15. Hirsch P., Gallikowski C. A., Siebert J., Peissl K., Kroppenstedt R., Schumann P., Stackebrandt E., Anderson R. 2004; Deinococcus frigens sp. nov., Deinococcus saxicola sp. nov., and Deinococcus marmoris sp. nov., low temperature and draught-tolerating, UV-resistant bacteria from continental Antarctica. Syst Appl Microbiol 27:636–645 [View Article][PubMed]
    [Google Scholar]
  16. Im W.-T., Jung H.-M., Ten L. N., Kim M. K., Bora N., Goodfellow M., Lim S., Jung J., Lee S. T. 2008; Deinococcus aquaticus sp. nov., isolated from fresh water, and Deinococcus caeni sp. nov., isolated from activated sludge. Int J Syst Evol Microbiol 58:2348–2353 [View Article][PubMed]
    [Google Scholar]
  17. Janssen P. H., Schuhmann A., Mörschel E., Rainey F. A. 1997; Novel anaerobic ultramicrobacteria belonging to the Verrucomicrobiales lineage of bacterial descent isolated by dilution culture from anoxic rice paddy soil. Appl Environ Microbiol 63:1382–1388[PubMed]
    [Google Scholar]
  18. Kämpfer P., Lodders N., Huber B., Falsen E., Busse H. J. 2008; Deinococcus aquatilis sp. nov., isolated from water. Int J Syst Evol Microbiol 58:2803–2806 [View Article][PubMed]
    [Google Scholar]
  19. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform 5:150–163 [View Article][PubMed]
    [Google Scholar]
  20. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [View Article][PubMed]
    [Google Scholar]
  21. Olsen G. J., Matsuda H., Hagstrom R., Overbeek R. 1994; fastDNAmL: a tool for construction of phylogenetic trees of DNA sequences using maximum likelihood. Comput Appl Biosci 10:41–48[PubMed]
    [Google Scholar]
  22. Peng F., Zhang L., Luo X.-S., Dai J., An H.-L., Tang Y.-L., Fang C.-X. 2009; Deinococcus xinjiangensis sp. nov., isolated from desert soil. Int J Syst Evol Microbiol 59:709–713 [View Article][PubMed]
    [Google Scholar]
  23. Rainey F. A., Ray K., Ferreira M., Gatz B. Z., Nobre M. F., Bagaley D., Rash B. A., Park M.-J., Earl A. M. et al. other authors 2005; Extensive diversity of ionizing-radiation-resistant bacteria recovered from Sonoran Desert soil and description of nine new species of the genus Deinococcus obtained from a single soil sample. Appl Environ Microbiol 71:5225–5235 [View Article][PubMed]
    [Google Scholar]
  24. Ross H. N. M., Grant W. D., Harris J. E. 1985; Lipids in archaebacterial taxonomy. In Chemical Methods in Bacterial Systematics pp. 280–300 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  25. Shashidhar R., Bandekar J. R. 2009; Deinococcus piscis sp. nov., a radiation-resistant bacterium isolated from a marine fish. Int J Syst Evol Microbiol 59:2714–2717 [View Article][PubMed]
    [Google Scholar]
  26. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231[PubMed]
    [Google Scholar]
  27. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  28. Weon H. Y., Kim B. Y., Schumann P., Son J. A., Jang J., Go S. J., Kwon S. W. 2007; Deinococcus cellulosilyticus sp. nov., isolated from air. Int J Syst Evol Microbiol 57:1685–1688 [View Article][PubMed]
    [Google Scholar]
  29. Worliczek H. L., Kämpfer P., Rosengarten R., Tindall B. J., Busse H. J. 2007; Polar lipid and fatty acid profiles – re-vitalizing old approaches as a modern tool for the classification of mycoplasmas?. Syst Appl Microbiol 30:355–370 [View Article][PubMed]
    [Google Scholar]
  30. Wu C., Lu X., Qin M., Wang Y., Ruan J. 1989; Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 16:176–178
    [Google Scholar]
  31. Xin H., Itoh T., Zhou P., Suzuki K., Nakase T. 2001; Natronobacterium nitratireducens sp. nov., a aloalkaliphilic archaeon isolated from a soda lake in China. Int J Syst Evol Microbiol 51:1825–1829 [View Article][PubMed]
    [Google Scholar]
  32. Ying J.-Y., Liu Z.-P., Wang B.-J., Dai X., Yang S.-S., Liu S.-J. 2007; Salegentibacter catena sp. nov., isolated from sediment of the South China Sea, and emended description of the genus Salegentibacter . Int J Syst Evol Microbiol 57:219–222 [View Article][PubMed]
    [Google Scholar]
  33. Yoo S. H., Weon H.-Y., Kim S.-J., Kim Y.-S., Kim B.-Y., Kwon S.-W. 2010; Deinococcus aerolatus sp. nov. and Deinococcus aerophilus sp. nov., isolated from air samples. Int J Syst Evol Microbiol 60:1191–1195 [View Article][PubMed]
    [Google Scholar]
  34. Yuan M.-L., Zhang W., Dai S., Wu J., Wang Y.-D., Tao T.-S., Chen M., Lin M. 2009; Deinococcus gobiensis sp. nov., an extremely radiation-resistant bacterium. Int J Syst Evol Microbiol 59:1513–1517 [View Article][PubMed]
    [Google Scholar]
  35. Zhang J.-Y., Liu X.-Y., Liu S.-J. 2010; Sphingomonas changbaiensis sp. nov., isolated from forest soil. Int J Syst Evol Microbiol 60:790–795 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.026567-0
Loading
/content/journal/ijsem/10.1099/ijs.0.026567-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error