1887

Abstract

In this study, the taxonomic positions of 19 isolates disclosed in a previous study were evaluated. Phylogenetic analysis based on 16S rDNA sequences partitioned these isolates into groups that were closely related (98·8–99·1 % similarity) to and , respectively. DNA–DNA hybridization experiments further showed that these groups had <70 % similarity to other species. Two novel species are proposed to accommodate these groups: sp. nov. (type strain, LMG 21557=CAIM 629) and sp. nov. (type strain, LMG 20362=CAIM 693). The DNA G+C content of both novel species is 45·6 mol%. Useful phenotypic features for discriminating and from other species include production of indole and acetoin, utilization of cellobiose, fermentation of amygdalin, melibiose and mannitol, -galactosidase and tryptophan deaminase activities and fatty acid composition.

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2003-09-01
2024-03-28
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References

  1. Acar J. F., Goldstein F. W. 1996; Disc susceptibility test. In Antibiotics in Laboratory Medicine , 4th edn. pp 1–51Edited by Lorian V. Baltimore: Williams & Wilkins;
    [Google Scholar]
  2. Alsina M., Blanch A. R. 1994; A set of keys for biochemical identification of environmental Vibrio species. J Appl Bacteriol 76:79–85 [CrossRef]
    [Google Scholar]
  3. Baumann P., Furniss A. L., Lee J. V. 1984; Genus I. Vibrio Pacini 1854. In Bergey's Manual of Systematic Bacteriology vol 1 pp 518–538Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  4. Ben-Haim Y., Thompson F. L., Thompson C. C., Cnockaert M. C., Hoste B., Swings J., Rosenberg E. 2003; Vibrio coralliilyticus sp. nov., a temperature-dependent pathogen of the coral Pocillopora damicornis . Int J Syst Evol Microbiol 53:309–315 [CrossRef]
    [Google Scholar]
  5. Bertone S., Giacomini M., Ruggiero C., Piccarolo C., Calegari L. 1996; Automated systems for identification of heterotrophic marine bacteria on the basis of their fatty acid composition. Appl Environ Microbiol 62:2122–2132
    [Google Scholar]
  6. Farmer J. J. III, Hickman-Brenner F. W. 1992; The genera Vibrio and Photobacterium . In The Prokaryotes. A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications . , 2nd edn. pp 2952–3011Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer-Verlag;
  7. Gomez-Gil B., Tron-Mayén L., Roque A., Turnbull J. F., Inglis V., Guerra-Flores A. L. 1998; Species of Vibrio isolated from hepatopancreas, haemolymph and digestive tract of a population of healthy juvenile Penaeus vannamei . Aquaculture 163:1–9 [CrossRef]
    [Google Scholar]
  8. Gomez-Gil B., Roque A., Turnbull J. F. 2000; The use and selection of probiotic bacteria for use in the culture of larval aquatic organisms. Aquaculture 191:259–270 [CrossRef]
    [Google Scholar]
  9. Gomez-Gil B., Roque A., Velasco-Blanco G. 2002; Culture of Vibrio alginolyticus C7b, a potential probiotic bacterium, with the microalga Chaetoceros muelleri . Aquaculture 211:43–48 [CrossRef]
    [Google Scholar]
  10. Heidelberg J. F., Heidelberg K. B., Colwell R. R. 2002a; Seasonality of Chesapeake Bay bacterioplankton species. Appl Environ Microbiol 68:5488–5497 [CrossRef]
    [Google Scholar]
  11. Heidelberg J. F., Heidelberg K. B., Colwell R. R. 2002b; Bacteria of the γ -subclass Proteobacteria associated with zooplankton in Chesapeake Bay. Appl Environ Microbiol 68:5498–5507 [CrossRef]
    [Google Scholar]
  12. Huys G., Vancanneyt M., Coopman R., Janssen P., Falsen E., Altwegg M., Kertsers K. 1994; Cellular fatty acid composition as a chemotaxonomic marker for the differentiation of phenospecies and hybridization groups in the genus Aeromonas . Int J Syst Bacteriol 44:651–658 [CrossRef]
    [Google Scholar]
  13. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp 21–132Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  14. Lambert M. A., Hickman-Brenner F. W., Farmer J. J. III, Moss C. W. 1983; Differentiation of Vibrionaceae species by their cellular fatty acid composition. Int J Syst Bacteriol 33:777–792 [CrossRef]
    [Google Scholar]
  15. Lipp E. K., Huq A., Colwell R. R. 2002; Effects of global climate on infectious disease: the cholera model. Clin Microbiol Rev 15:757–770 [CrossRef]
    [Google Scholar]
  16. Macián M. C., Ludwig W., Schleifer K.-H., Garay E., Pujalte M. J. 2000; Vibrio pelagius : differences of the type strain deposited at various culture collections. Syst Appl Microbiol 23:373–375 [CrossRef]
    [Google Scholar]
  17. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  18. Moss S. M., LeaMaster B. R., Sweeney J. N. 2000; Relative abundance and species composition of gram-negative, aerobic bacteria associated with the gut of juvenile white shrimp Litopenaeus vannamei reared in oligotrophic well water and eutrophic pond water. J World Aquac Soc 31:255–263 [CrossRef]
    [Google Scholar]
  19. Pitcher D. G., Saunders N. A., Owen R. J. 1989; Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8:151–156 [CrossRef]
    [Google Scholar]
  20. Pujalte M. J., Ortigosa M., Urdaci M.-C., Garay E., Grimont P. A. D. 1993; Vibrio mytili sp. nov., from mussels. Int J Syst Bacteriol 43:358–362 [CrossRef]
    [Google Scholar]
  21. Raguénès G., Christen R., Guezennec J., Pignet P., Barbier G. 1997; Vibrio diabolicus sp. nov., a new polysaccharide-secreting organism isolated from a deep-sea hydrothermal vent polychaete annelid, Alvinella pompejana. Int J Syst Bacteriol 47989–995 [CrossRef]
    [Google Scholar]
  22. Ruimi R., Breittmayer V., Elbaze P., Lafay B., Boussemart O., Gauthier M., Christen R. 1994; Phylogenetic analysis and assessment of the genera Vibrio , Photobacterium , Aeromonas , and Plesiomonas deduced from small-subunit rRNA sequences. Int J Syst Bacteriol 44:416–426 [CrossRef]
    [Google Scholar]
  23. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  24. Thompson F. L., Hoste B., Vandemeulebroecke K., Swings J. 2001; Genomic diversity amongst Vibrio isolates from different sources determined by fluorescent amplified fragment length polymorphism. Syst Appl Microbiol 24:520–538 [CrossRef]
    [Google Scholar]
  25. Thompson F. L., Hoste B., Vandemeulebroecke K., Engelbeen K., Denys R., Swings J. 2002; Vibrio trachuri Iwamoto et al . 1995 is a junior synonym of Vibrio harveyi (Johnson and Shunk 1936) Baumann et al . 1981. Int J Syst Evol Microbiol 52:973–976 [CrossRef]
    [Google Scholar]
  26. Thompson F. L., Li Y., Gomez-Gil B.8 other authors 2003; Vibrio neptunius sp. nov., Vibrio brasiliensis sp. nov. and Vibrio xuii sp. nov., isolated from the marine aquaculture environment (bivalves, fish, rotifers and shrimps. Int J Syst Evol Microbiol 53:245–252 [CrossRef]
    [Google Scholar]
  27. Vandamme P., Segers P., Ryll M.8 other authors 1998; Pelistega europaea gen. nov., sp. nov., a bacterium associated with respiratory disease in pigeons: taxonomic structure and phylogenetic allocation. Int J Syst Bacteriol 48:431–440 [CrossRef]
    [Google Scholar]
  28. Vandenberghe J., Verdonck L., Robles-Arozarena R.7 other authors 1999; Vibrios associated with Litopenaeus vannamei larvae, postlarvae, broodstock, and hatchery probionts. Appl Environ Microbiol 65:2592–2597
    [Google Scholar]
  29. Verschuere L., Rombaut G., Sorgeloos P., Verstraete W. 2000; Probiotic bacteria as biological control agents in aquaculture. Microbiol Mol Biol Rev 64:655–671 [CrossRef]
    [Google Scholar]
  30. Willems A., Doignon-Bourcier F., Goris J., Coopman R., de Lajudie P., De Vos P., Gillis M. 2001; DNA–DNA hybridization study of Bradyrhizobium strains. Int J Syst Evol Microbiol 51:1315–1322
    [Google Scholar]
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