1887

Abstract

A Gram-reaction-positive aerobic actinomycete, designated strain IM17-1, was isolated from a honey bee () hive in Chiang Mai Province, Thailand. The strain formed a branched substrate mycelium and mature aerial mycelium bore short chains of arthrospores with warty surfaces. The cell wall contained -2,6-diaminopimelic acid (cell-wall type III) and the whole cell sugars were fucose, galactose, glucose, madurose, mannose and ribose. The major isoprenoid quinone was hexahydrogenated menaquinone with nine isoprene units and the predominant cellular fatty acids were C (33.8 %), Cω9 (32.7 %), summed feature 3 (Cω7 and/or iso-C 2-OH) (8.7 %) and 10-methyl C (8.2 %). The phospholipids were diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. These morphological and chemotaxonomic characteristics were consistent with the classification of IM17-1 within the genus . Based on 16S rRNA gene sequence analysis, strain IM17-1 was closely related to the type strains of subsp. (98.1 %) and subsp. (98.6 %); however, it represented a distinct phylogenetic lineage from the other species within this genus. The unique genetic characteristics were reaffirmed by low levels of DNA–DNA relatedness between strain IM17-1 and the two most closely related type strains, subsp. JCM 3308 (56.5±4.9 %) and subsp. JCM 3309 (31.0±22.6 %), and further supported the proposal of IM17-1 as a novel species. Strain IM17-1 ( = JCM 16576  = TISTR 1980) thus represents a novel species of the genus , for which the name sp. nov. is proposed. In addition, the genotypic and phenotypic data suggested the reclassification of subsp. Gauze 1987 as a separate species, sp. nov., comb. nov.

Funding
This study was supported by the:
  • Strategic Scholarships for Frontier Research Network
  • Office of the Higher Education Commission and the Graduate School, Chiang Mai University
  • Thailand Research Fund (Award RSA5280010)
  • Hitachi Scholarship Foundation
  • Office of the Higher Education Commission
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2011-09-01
2024-03-28
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References

  1. Ara I., Matsumoto A., Bakir M. A., Kudo T., Omura S., Takahashi Y. 2008; Actinomadura bangladeshensis sp. nov. and Actinomadura chokoriensis sp. nov.. Int J Syst Evol Microbiol 58:1653–1659 [View Article][PubMed]
    [Google Scholar]
  2. Asano K., Kawamoto I. 1986; Catellatospora, a new genus of the Actinomycetales . Int J Syst Bacteriol 36:512–517 [View Article]
    [Google Scholar]
  3. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230[PubMed] [CrossRef]
    [Google Scholar]
  4. Collins M. D., Faulkner M., Keddie R. M. 1984; Menaquinone composition of some spore forming actinomycetes. Syst Appl Microbiol 5:20–29 [CrossRef]
    [Google Scholar]
  5. Cook A. E., Roes M., Meyers P. R. 2005; Actinomadura napierensis sp. nov., isolated from soil in South Africa. Int J Syst Evol Microbiol 55:703–706 [View Article][PubMed]
    [Google Scholar]
  6. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
    [Google Scholar]
  7. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  8. Gauze G. F., Preobrazhenskaia T. P., Lavrova N. V., Ukholina R. S., Kochetkova N. V. 1975; [Actinomadura cremea var. rifamycini, a producer of rifamycin O]. Antibiotiki 20:963–966 (in Russian) [PubMed]
    [Google Scholar]
  9. Gauze G. F., Terekhova L. P., Galatenko O. A., Preobrazhenskaya T. P., Borisova V. N., Federova G. B. 1987; Actinomadura cremea subsp. rifamicini subsp. nov. In Validation of the publication of new names and new combinations previously effectively published outside the IJSB: List no. 23. Int J Syst Bacteriol 37:179–180 [View Article]
    [Google Scholar]
  10. Howey R. T., Lock C. M., Moore L. V. H. 1990; Subspecies names automatically created by Rule 46. Int J Syst Bacteriol 40:317–319 [View Article]
    [Google Scholar]
  11. Itoh T., Kudo T., Parenti F., Seino A. 1989; Amended description of the genus Kineosporia, based on chemotaxonomic and morphological studies. Int J Syst Bacteriol 39:168–173 [View Article]
    [Google Scholar]
  12. Jacobson E., Grauville W. C., Fogs C. E. 1958 Color Harmony Manual, 4th edn. Chicago: Container Corporation of America;
    [Google Scholar]
  13. Kroppenstedt R. M. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics pp. 173–199 Edited by Goodfellow M., Minnikin D. E. London: Academic Press, Ltd;
    [Google Scholar]
  14. Kroppenstedt R. M., Goodfellow M. 2006; The family Thermomonosporaceae: Actinocorallia, Actinomadura, Spirillospora and Thermomonospora . In The Prokaryotes, 3rd edn. vol. 3 pp. 682–724 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E. New York, NY: Springer; [View Article]
    [Google Scholar]
  15. Kroppenstedt R. M., Stackebrandt E., Goodfellow M. 1990; Taxonomic revision of the actinomycete genera Actinomadura and Microtetraspora . Syst Appl Microbiol 13:148–160 [CrossRef]
    [Google Scholar]
  16. Küster E., Williams S. T. 1964; Selection of media for isolation of streptomycetes. Nature 202:928–929 [View Article][PubMed]
    [Google Scholar]
  17. Lechevalier H. A., Lechevalier M. P. 1968; A critical evaluation of the genera of aerobic actinomycetes. In The Actinomycetales pp. 393–405 Edited by Prauser H. Jena: VEB Gustav Fischer Verlag;
    [Google Scholar]
  18. Lechevalier M. P., Lechevalier H. A. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443 [View Article]
    [Google Scholar]
  19. Lechevalier M. P., Stern A. E., Lechevalier H. A. 1981; Phospholipids in the taxonomy of actinomycetes. Zentralbl Bakteriol Microbiol Hyg Abt 1:Suppl 11111–116
    [Google Scholar]
  20. Mikami H., Ishida Y. 1983; Post-column fluorometric detection of reducing sugar in high-performance liquid chromatography using arginine. Bunseki Kagaku 32:E207–E210 [View Article]
    [Google Scholar]
  21. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
    [Google Scholar]
  22. Nakajima Y., Kitpreechavanich V., Suzuki K., Kudo T. 1999; Microbispora corallina sp. nov., a new species of the genus Microbispora isolated from Thai soil. Int J Syst Bacteriol 49:1761–1767 [View Article][PubMed]
    [Google Scholar]
  23. Preobrazhenskaya T. P., Lavrova N. V., Ukholina R. S., Nechaeva N. P. 1975; [Isolation of new species of the genus Actinomadura on selective media with streptomycin and bruneomycin]. Antibiotiki 20:404–409 (in Russian) [PubMed]
    [Google Scholar]
  24. Promnuan Y., Kudo T., Chantawannakul P. 2009; Actinomycetes isolated from beehives in Thailand. World J Microbiol Biotechnol 25:1685–1689 [View Article]
    [Google Scholar]
  25. Qin S., Zhao G. Z., Li J., Zhu W. Y., Xu L. H., Li W. J. 2009; Actinomadura flavalba sp. nov., an endophytic actinomycete isolated from leaves of Maytenus austroyunnanensis. Int J Syst Evol Microbiol 59:2453–2457 [View Article]
    [Google Scholar]
  26. Reader U., Broda P. 1985; Rapid preparation of DNA from filamentous fungi. Lett Appl Microbiol 1:17–20 [View Article]
    [Google Scholar]
  27. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  28. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc..
  29. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [View Article]
    [Google Scholar]
  30. Stackebrandt E., Ebers J. 2006; Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33:152–155
    [Google Scholar]
  31. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov.. Int J Syst Bacteriol 47:479–491 [View Article]
    [Google Scholar]
  32. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231[PubMed]
    [Google Scholar]
  33. Takahashi K., Nei M. 2000; Efficiencies of fast algorithms of phylogenetic inference under the criteria of maximum parsimony, minimum evolution, and maximum likelihood when a large number of sequences are used. Mol Biol Evol 17:1251–1258[PubMed] [CrossRef]
    [Google Scholar]
  34. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
    [Google Scholar]
  35. Tamaoka J., Katayama-Fujimura Y., Kuraishi H. 1983; Analysis of bacterial menaquinone mixtures by high-performance liquid chromatography. J Appl Microbiol 54:31–36 [View Article]
    [Google Scholar]
  36. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  37. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  38. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
  39. Yassin A. F., Spröer C., Siering C., Klenk H.-P. 2010; Actinomadura sputi sp. nov., isolated from the sputum of a patient with pulmonary infection. Int J Syst Evol Microbiol 60:149–153 [View Article][PubMed]
    [Google Scholar]
  40. Zhi X. Y., Li W. J., Stackebrandt E. 2009; An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. Int J Syst Evol Microbiol 59:589–608 [View Article][PubMed]
    [Google Scholar]
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