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Volume 61, Issue 12

sp. nov., a moderate halophile isolated from a sea urchin Free

Abstract

A novel Gram-staining-positive, moderately halophilic, facultatively alkaliphilic, non-motile, catalase-positive, oxidase-negative, endospore-forming, facultatively anaerobic rod, designated JSM 076093, was isolated from a sea urchin () collected from Naozhou Island in the South China Sea. Growth occurred with 0.5–25 % (w/v) NaCl (optimum 5–8 %) and at pH 6.0–10.5 (optimum pH 8.0) and 5–40 °C (optimum 30–35 °C). -Diaminopimelic acid was present in the cell-wall peptidoglycan. The predominant respiratory quinone was menaquinone 7 and the polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid. The major cellular fatty acids (>10 % of the total) were anteiso-C, anteiso-C, iso-C and iso-C. The genomic DNA G+C content was 38.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JSM 076093 belonged to the genus and was related most closely to SW-72 (99.1 % 16S rRNA gene sequence similarity) and KMM 3737 (97.3 %). The combination of results from the phylogenetic analysis, DNA–DNA hybridization and phenotypic and chemotaxonomic characterization supported the conclusion that strain JSM 076093 represents a novel species of the genus , for which the name sp. nov. is proposed, with JSM 076093 ( = DSM 23007 = KCTC 13710) as the type strain.

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Funding
This study was supported by the:
  • National Basic Research Program of China (Award 2010CB833800)
  • National Natural Science Foundation of China (Award 30970008 and 30970007)
  • state-level public welfare scientific research institutes for basic R&D special fund (Award IMBF200915)
  • International Cooperation Research Program of Yunnan Province (Award 2009AC017)
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Bacillus hemicentroti sp. nov., a moderate halophile isolated from a sea urchin
Int J Syst Evol Microbiol 61, 2950 (2011); https://doi.org/10.1099/ijs.0.026732-0
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