1887

Abstract

is an industrially important micro-organism because of its ability to produce endospores which resist ultra-high temperature (UHT) and industrial sterilization processes. It was described by Pettersson (1996) [Pettersson, B., Lembke, F., Hammer, P., Stackebrandt, E. & Priest, F. G. (1996). , 759–764] based on seven genetically homogeneous isolates all from UHT milk. , the closest phylogenetic neighbour of , was described by Kuhnigk (1995) [Kuhnigk, T., Borst, E.-M., Breunig, A., König, H., Collins, M. D., Hutson, R. A. & Kämpfer, P. (1995). , 699–706] based on a single strain, isolated from the hindgut of the termite A polyphasic study of a heterogeneous collection of and strains isolated from various sources and geographical origins led to an emended description of both species. Additional data presented are the results of fatty acid, quinone and/or cell wall (polar lipid) analyses. DNA–DNA hybridization confirmed 3 subgroups of strains obtained after SDS-PAGE analysis of cellular proteins as . One named strain (R-7489) was reclassified as a strain. The phenotypic profiles of both species were rather heterogeneous, sometimes different from the original descriptions and did not differ in a large number of characteristics, although generally gave stronger reactions in its positive tests than did ; the variable and weak reactions for both organisms with some substrates blurred the distinction between the two. However, differences in polar lipid, SDS-PAGE and menaquinone profiles clearly allow distinction between the two species.

Funding
This study was supported by the:
  • Fund for Scientific Research - Flanders (Award G.0156.02)
  • FWO-Vlaanderen
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2012-02-01
2024-03-28
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