%0 Journal Article %A Kaewkla, Onuma %A Franco, Christopher M. M. %T Flindersiella endophytica gen. nov., sp. nov., an endophytic actinobacterium isolated from the root of Grey Box, an endemic eucalyptus tree %D 2011 %J International Journal of Systematic and Evolutionary Microbiology, %V 61 %N 9 %P 2135-2140 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.026757-0 %I Microbiology Society, %X A novel endophytic actinobacterium, designated strain EUM 378T, was isolated from the surface-sterilized root tissue of Eucalyptus microcarpa, a eucalyptus tree known as Grey Box. Phylogenetic evaluation based on 16S rRNA gene sequence analysis, including alignment with taxon-specific 16S rRNA gene signature nucleotides, placed this isolate as a member of the family Nocardioidaceae. Strain EUM 378T showed >5.5 % 16S rRNA gene sequence divergence from other members of this family and was related most closely to Actinopolymorpha alba YIM 48868T (94.2 %) and Actinopolymorpha singaporensis IM 7744T (94.4 %). This Gram-positive, aerobic actinobacterium has well-developed substrate mycelia that fragment into small rods. Chemotaxonomic data revealed that the cell wall contains ll-diaminopimelic acid, ribose, glucose and rhamnose. MK-10(H6) is the predominant menaquinone. Chemotaxonomic and phylogenetic evidence confirmed that strain EUM 378T represents a novel species of a new genus, for which the name Flindersiella endophytica gen. nov., sp. nov. is proposed. The type strain is EUM 378T ( = DSM 45355T = ACM 5289T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.026757-0