@article{mbs:/content/journal/ijsem/10.1099/ijs.0.02677-0, author = "Valverde, Angel and Velázquez, Encarna and Gutiérrez, Carmen and Cervantes, Emilio and Ventosa, Antonio and Igual, José-Mariano", title = "Herbaspirillum lusitanum sp. nov., a novel nitrogen-fixing bacterium associated with root nodules of Phaseolus vulgaris", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2003", volume = "53", number = "6", pages = "1979-1983", doi = "https://doi.org/10.1099/ijs.0.02677-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.02677-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "TP-RAPD, two-primer randomly amplified polymorphic DNA", abstract = "Several bacterial strains were isolated from root nodules of Phaseolus vulgaris plants grown in a soil from Portugal. The strains were Gram-negative, aerobic, curved rod-shaped and motile. The isolates were catalase- and oxidase-positive. The TP-RAPD (two-primer randomly amplified polymorphic DNA) patterns of all strains were identical, suggesting that they belong to the same species. The complete 16S rDNA sequence of a representative strain was obtained and phylogenetic analysis based on the neighbour-joining method indicated that this bacterium belongs to the β-Proteobacteria and that the closest related genus is Herbaspirillum. The DNA G+C content ranged from 57·9 to 61·9 mol%. Growth was observed with many different carbohydrates and organic acids including caprate, malate, citrate and phenylacetate. No growth was observed with maltose, meso-inositol, meso-erythritol or adipate as sole carbon source. According to the phenotypic and genotypic data obtained in this work, the bacterium represents a novel species of the genus Herbaspirillum, and the name Herbaspirillum lusitanum sp. nov. is proposed. The type strain is P6-12T (=LMG 21710T=CECT 5661T).", }