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Volume 61, Issue 7

gen. nov., sp. nov., a new member of the family Free

Abstract

Two extremely halophilic archaeal strains, TBN21 and TBN49, were isolated from the Taibei marine solar saltern near Lianyungang city, Jiangsu province, China. Cells of the two strains were pleomorphic and Gram-negative and colonies were red. Strains TBN21 and TBN49 were able to grow at 25–50 °C (optimum 37 °C), at 1.4–5.1 M NaCl (optimum 3.4–3.9 M) and at pH 5.5–9.5 (optimum pH 7.0–7.5) and neither strain required Mg for growth. Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 8 % (w/v). The major polar lipids of the two strains were phosphatidic acid, phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and eight glycolipids; three of these glycolipids (GL3, GL4 and GL5) were chromatographically identical to sulfated mannosyl glucosyl diether (S-DGD-1), galactosyl mannosyl glucosyl diether (TGD-1) and mannosyl glucosyl diether (DGD-1), respectively. Phylogenetic analysis revealed that strains TBN21 and TBN49 formed a distinct clade with their closest relative, JCM 9908 (89.0–89.5 % 16S rRNA gene sequence similarity). The DNA G+C contents of strains TBN21 and TBN49 were 64.8 and 62.7 mol%, respectively. DNA–DNA hybridization between strains TBN21 and TBN49 was 90.1 %. The phenotypic, chemotaxonomic and phylogenetic properties suggest that strains TBN21 and TBN49 represent a novel species in a new genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is TBN21 ( = CGMCC 1.10329  = JCM 16809).

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Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 30970006)
© 2011 IUMS
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2011-07-01
2024-04-18
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Halolamina pelagica gen. nov., sp. nov., a new member of the family Halobacteriaceae
Int J Syst Evol Microbiol 61, 1617 (2011); https://doi.org/10.1099/ijs.0.026799-0
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