1887

Abstract

A taxonomic study was carried out on two bacterial strains, PCP11 and PCP104, isolated from a tidal flat of the Yellow Sea, Korea. Comparative 16S rRNA gene sequence studies showed that these strains belonged to the family , phylum . Strains PCP11 and PCP104 shared 99.4 % sequence similarity and were related most closely to KMM 3525 (95.8 and 96.0 % sequence similarity, respectively). Members of the genera , , and were the next closest relatives of the new isolates, with sequence similarities ≤91 %. The two isolates were Gram-staining-negative, strictly aerobic, gliding bacteria. They grew in the presence of 1–5 % NaCl, at pH 5.5–8.5 and at 4–35 °C. Strains PCP11 and PCP104 shared a number of physiological and biochemical properties with KMM 3525, but they differed from this strain in the hydrolysis of biopolymers and in the production of carotenoid and flexirubin-type pigments. Both strains possessed iso-C, summed feature 4 (Cω7 and/or iso-C 2-OH) and C as major cellular fatty acids. The major respiratory quinone was menaquinone 7 (MK-7). The G+C contents of the genomic DNA of strains PCP11 and PCP104 were 39.6 and 41.9 mol%, respectively. On the basis of phenotypic data and phylogenetic inference, it is proposed that the two isolates represent a novel species, sp. nov., with strain PCP11 ( = KACC 14523  = JCM 16588) as the type strain. Emended descriptions of the genus and are also proposed.

Funding
This study was supported by the:
  • Korean Ministry of Education, Science and Technology
  • Regional Core Research Program/Chungbuk BIT Research-Oriented University Consortium
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.026849-0
2011-08-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/8/1994.html?itemId=/content/journal/ijsem/10.1099/ijs.0.026849-0&mimeType=html&fmt=ahah

References

  1. Agarwal S., Hunnicutt D. W., McBride M. J. 1997; Cloning and characterization of the Flavobacterium johnsoniae (Cytophaga johnsonae) gliding motility gene, gldA . Proc Natl Acad Sci U S A 94:12139–12144 [View Article][PubMed]
    [Google Scholar]
  2. Bernardet J.-F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [View Article][PubMed]
    [Google Scholar]
  3. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  4. Euzéby J. P. 1997; List of bacterial names with standing in nomenclature: a folder available on the Internet. Int J Syst Bacteriol 47:590–592 http://www.bacterio.cict.fr [View Article][PubMed]
    [Google Scholar]
  5. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
    [Google Scholar]
  6. González J. M., Mayer F., Moran M. A., Hodson R. E., Whitman W. B. 1997; Microbulbifer hydrolyticus gen. nov., sp. nov., and Marinobacterium georgiense gen. nov., sp. nov., two marine bacteria from a lignin-rich pulp mill waste enrichment community. Int J Syst Bacteriol 47:369–376 [View Article][PubMed]
    [Google Scholar]
  7. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  8. Hiraishi A., Ueda Y., Ishihara J., Mori T. 1996; Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 42:457–469 [View Article]
    [Google Scholar]
  9. Kimura M. 1983 The Neutral Theory of Molecular Evolution Cambridge: Cambridge University; [CrossRef]
    [Google Scholar]
  10. Ludwig W., Euzéby J., Whitman W. B. 2008; Draft taxonomic outline of the Bacteroidetes, Planctomycetes, Chlamydiae, Spirochaetes, Fibrobacteres, Fusobacteria, Acidobacteria, Verrucomicrobia, Dictyoglomi, and Gemmatimonadetes . http://www.bergeys.org/outlines/Bergeys_Vol_4_Outline.pdf
  11. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G + C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  12. Nedashkovskaya O. I., Suzuki M., Vysotskii M. V., Mikhailov V. V. 2003; Reichenbachia agariperforans gen. nov., sp. nov., a novel marine bacterium in the phylum Cytophaga–Flavobacterium–Bacteroides . Int J Syst Evol Microbiol 53:81–85 [View Article][PubMed]
    [Google Scholar]
  13. Nedashkovskaya O. I., Kim S. B., Suzuki M., Shevchenko L. S., Lee M. S., Lee K. H., Park M. S., Frolova G. M., Oh H. W. et al. 2005; Pontibacter actiniarum gen. nov., sp. nov., a novel member of the phylum ‘Bacteroidetes’, and proposal of Reichenbachiella gen. nov. as a replacement for the illegitimate prokaryotic generic name Reichenbachia Nedashkovskaya et al. 2003. Int J Syst Evol Microbiol 55:2583–2588 [View Article][PubMed]
    [Google Scholar]
  14. Park S. J., Kang C. H., Rhee S. K. 2006; Characterization of the microbial diversity in a Korean solar saltern by 16S rRNA gene analysis. J Microbiol Biotechnol 16:1640–1645
    [Google Scholar]
  15. Reichenbach H. 1989; The order Cytophagales Leadbetter 1974, 99AL . In Bergey’s Manual of Systematic Bacteriology vol. 3 pp. 2011–2073 Edited by Staley J. T., Bryant M. P., Pfennig N., Holt J. C. Baltimore: Williams & Wilkins;
    [Google Scholar]
  16. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  17. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  18. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  19. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  20. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  21. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703[PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.026849-0
Loading
/content/journal/ijsem/10.1099/ijs.0.026849-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error