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Volume 61, Issue 12

gen. nov., sp. nov., a member of the phylum isolated from sediment in a sea cucumber farm Free

Abstract

Three novel aerobic, elliptic bacteria, designated DQHS4, DQHS8 and DQHS15, were isolated from sediment of a seashore pond for sea cucumber culture in Jimo, Qingdao, on the east coast of China. Cells were Gram-, oxidase- and catalase-negative. All three strains grew at 15–42 °C, pH 5–9 and NaCl concentrations between 0.5 and 10 %. DNA–DNA hybridization experiments revealed high (>85 %) relatedness among the three novel isolates and suggested that the strains constitute a single species. Comparative 16S rRNA gene sequence analysis indicated that these bacteria had less than 90 % similarity to all described species of the phylum ; the closest relative of the three isolates was F2, sharing only 89.6 % sequence similarity. The major cellular fatty acids were iso-C 3-OH (19.8–20.0 %), iso-C (16.9–17.3 %), anteiso-C B and/or iso-C I (7.4–8.7 %), C 2-OH (8.4 %), anteiso-C (8.2–8.6 %) and Cω6 (5.6–6.0 %). The major respiratory quinone was menaquinone-7 (MK-7) and the DNA G+C content was 41.8–43.5 mol%. Based on the distinct phylogenetic position and the combination of genotypic, phenotypic and chemotaxonomic characteristics, these three strains were considered to represent a novel species of a new genus in the phylum , for which the name gen. nov., sp. nov. is proposed. The type strain of is DQHS4 ( = CGMCC 1.9156  = NCCB 100301  = LMG 25367).

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Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 41006103)
© 2011 IUMS
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2011-12-01
2024-04-16
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Sunxiuqinia elliptica gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from sediment in a sea cucumber farm
Int J Syst Evol Microbiol 61, 2885 (2011); https://doi.org/10.1099/ijs.0.026971-0
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