1887

Abstract

A polyphasic taxonomic study (employing whole-cell protein and fatty acid analyses, 16S rDNA sequencing, DNA–DNA hybridization, determination of DNA G+C content, antibiotic susceptibility testing and extensive phenotypic characterization) was performed on 10 isolates that appeared to be related to . The isolates were recovered from diverse environments that included human clinical samples. 16S rDNA sequence analysis indicated that these isolates belonged to the genus . Whole-cell protein analysis distinguished two groups, which were confirmed by DNA–DNA hybridization. Based on the results of this study, the organisms were classified as two novel species, sp. nov. (type strain, LMG 6003) and sp. nov. (type strain, LMG 5911). can be distinguished from by its ability to grow on acetamide and to assimilate mesaconate and aconitate, and by its inability to assimilate diaminobutane. Various tests allow the differentiation of both novel species from other species, including growth on acetamide, denitrification and assimilation of -glucose, -xylose, mesaconate, aconitate and diaminobutane.

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2003-11-01
2024-03-19
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References

  1. Coenye T., Falsen E., Vancanneyt M., Hoste B., Govan J. R. W., Kersters K., Vandamme P. 1999; Classification of Alcaligenes faecalis -like isolates from the environment and human clinical samples as Ralstonia gilardii sp. nov. Int J Syst Bacteriol 49:405–413 [CrossRef]
    [Google Scholar]
  2. Coenye T., Vancanneyt M., Cnockaert M., Falsen E., Swings J., Vandamme P. 2003; Kerstersia gyiorum gen. nov., sp. nov., a novel Alcaligenes faecalis -like organism isolated from human clinical samples, and reclassification of Alcaligenes denitrificans Rüger and Tan 1983 as Achromobacter denitrificans comb. nov. Int J Syst Evol Microbiol 53:1825–1831 [CrossRef]
    [Google Scholar]
  3. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  5. Foss S., Heyen U., Harder J. 1998; Alcaligenes defragrans sp. nov., description of four strains isolated on alkenoic monoterpenes ((+)-menthene, α -pinene, 2-carene, and α -phellandrene) and nitrate. Syst Appl Microbiol 21:237–244 [CrossRef]
    [Google Scholar]
  6. Kersters K., De Ley J. 1984; Genus Alcaligenes Castellani and Chalmers 1919, 936AL . In Bergey's Manual of Systematic Bacteriology vol 1 pp 361–373Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  7. Kersters K., Hinz K.-H., Hertle A., Segers P., Lievens A., Siegmann O., De Ley J. 1984; Bordetella avium sp. nov., isolated from the respiratory tracts of turkeys and other birds. Int J Syst Bacteriol 34:56–70 [CrossRef]
    [Google Scholar]
  8. Kiredjian M., Holmes B., Kersters K., Guilvout I., De Ley J. 1986; Alcaligenes piechaudii , a new species from human clinial specimens and the environment. Int J Syst Bacteriol 36:282–287 [CrossRef]
    [Google Scholar]
  9. Liu L., Coenye T., Burns J. L., Whitby P. W., Stull T. L., LiPuma J. J. 2002; Ribosomal DNA-directed PCR for identification of Achromobacter ( Alcaligenes ) xylosoxidans recovered from sputum samples from cystic fibrosis patients. J Clin Microbiol 40:1210–1213 [CrossRef]
    [Google Scholar]
  10. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  11. National Committee for Clinical Laboratory Standards 1995 NCCLS Procedures vol. 15no. 14 Villanova, PA: NCCLS;
    [Google Scholar]
  12. Pitcher D. G., Saunders N. A., Owen R. J. 1989; Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8:151–156 [CrossRef]
    [Google Scholar]
  13. Pot B., Vandamme P., Kersters K. 1994; Analysis of electrophoretic whole-organism protein fingerprints. In Chemical Methods in Prokaryotic Systematics pp 493–521Edited by Goodfellow M., O'Donnell A. G. J. Chichester: Wiley;
    [Google Scholar]
  14. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  15. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  16. Vancanneyt M., Vandamme P., Kersters K. 1995; Differentiation of Bordetella pertussis , B. parapertussis , and B. bronchiseptica by whole-cell protein electrophoresis and fatty acid analysis. Int J Syst Bacteriol 45:843–847 [CrossRef]
    [Google Scholar]
  17. Vandamme P., Vancanneyt M., Pot B. 10 other authors 1992; Polyphasic taxonomic study of the emended genus Arcobacter with Arcobacter butzleri comb. nov. and Arcobacter skirrowii sp. nov., an aerotolerant bacterium isolated from veterinary specimens. Int J Syst Bacteriol 42:344–356 [CrossRef]
    [Google Scholar]
  18. Vandamme P., Gillis M., Vancanneyt M., Hoste B., Kersters K., Falsen E. 1993; Moraxella lincolnii sp. nov., isolated from the human respiratory tract, and reevaluation of the taxonomic position of Moraxella osloensis . Int J Syst Bacteriol 43:474–481 [CrossRef]
    [Google Scholar]
  19. Vandamme P., Hommez J., Vancanneyt M., Monsieurs M., Hoste B., Cookson B., Wirsing von König C. H., Kersters K., Blackall P. J. 1995; Bordetella hinzii sp. nov., isolated from poultry and humans. Int J Syst Bacteriol 45:37–45 [CrossRef]
    [Google Scholar]
  20. Vandamme P., Heyndrickx M., Vancanneyt M., Hoste B., De Vos P., Falsen E., Kersters K., Hinz K.-H. 1996; Bordetella trematum sp. nov., isolated from wounds and ear infections in humans, and reassessment of Alcaligenes denitrificans Rüger and Tan 1983. Int J Syst Bacteriol 46:849–858 [CrossRef]
    [Google Scholar]
  21. Yabuuchi E., Yano I. 1981; Achromobacter gen. nov. and Achromobacter xylosoxidans (ex Yabuuchi and Ohyama 1971). nom. rev. Int J Syst Bacteriol 31:477–478 [CrossRef]
    [Google Scholar]
  22. Yabuuchi E., Kawamura Y., Kosako Y., Ezaki T. 1998; Emendation of genus Achromobacter and Achromobacter xylosoxidans (Yabuuchi and Yano) and proposal of Achromobacter ruhlandii (Packer and Vishniac) comb. nov., Achromobacter piechaudii (Kiredjian et al .) comb. nov., and Achromobacter xylosoxidans subsp. denitrificans (Rüger and Tan) comb. nov. Microbiol Immunol 42429–438 [CrossRef]
    [Google Scholar]
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