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Abstract

Two novel vibrio isolates (R-40492 and R-40493) originating from the zoanthid in Brazil in 2005 were taxonomically characterized by means of a polyphasic approach comprising multilocus sequence analysis (MLSA), DNA–DNA hybridization (DDH), Δ analysis and phenotypic characterization. Phylogenetic analysis based on 16S rRNA gene sequences showed that R-40492 and R-40493 fell within the genus and were most closely related to each other with 99 % similarity; similarities of these two novel isolates towards LMG 20536, LMG 20984, LMG 3896, LMG 25238 and LMG 20546 varied between 97.1 and 98.5 %. DDH experiments showed that the two isolates had less than 15 % relatedness to the phylogenetically most closely related species. R-40492 and R-40493 had 55–57 % relatedness to each other. The Δ between R-40492 and R-40493 was 6.12 °C. In addition, MLSA of concatenated sequences (16S rRNA, , , , , , and ; 6035 bp in length) showed that the two novel isolates formed a separate branch with less than 92 % concatenated gene sequence similarity towards known species of vibrios. Two novel species are proposed to accommodate these novel isolates, namely sp. nov. (type strain, R-40492 = LMG 25438 = CAIM 1454) and sp. nov. (type strain, R-40493 = LMG 25439 = CAIM 1455).

Funding
This study was supported by the:
  • FAPERJ, US Embassy
  • CAPES
  • FAPESP
  • CNPq
  • IFS
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2011-12-01
2024-03-29
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