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Volume 61, Issue 12

sp. nov., isolated from compost samples Free

Abstract

Two unusual, Gram-negative, catalase- and oxidase-positive rods, designated C2 and C5, were isolated from compost samples. Comparative 16S rRNA gene sequencing studies demonstrated that both isolates were members of the genus and belonged to the group. Strain C2 was most closely related to 1N and R-20821 (97.9 and 97.8 % 16S rRNA gene sequence similarity, respectively). However, phylogenetic analysis based on gene sequences revealed that both isolates could be discriminated from members of the group that exhibited >97 % 16S rRNA gene sequence similarity. The DNA G+C content of strain C2 was 61.5 mol%. The major fatty acids of strain C2 were a summed feature (Cω7 and/or iso-C 2-OH), Cω7/12/9, C and C, which supported the isolates’ affiliation with the genus . Moreover, strain C2 could be distinguished from its closest phylogenetic neighbours of the genus by DNA–DNA hybridization studies and biochemical tests. On the basis of both phenotypic and phylogenetic findings, it is proposed that the isolates be classified as a novel species, with the name sp. nov. The type strain is C2 ( = CECT 7516 = CCUG 59231).

  • Published Online:
Funding
This study was supported by the:
  • Spanish Ministry of Education and Science (Award CTM-2007-66306-C02-01)
  • Complutense University
© 2011 IUMS
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2011-12-01
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Pseudomonas composti sp. nov., isolated from compost samples
Int J Syst Evol Microbiol 61, 2962 (2011); https://doi.org/10.1099/ijs.0.027086-0
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