1887

Abstract

A pigmented, slowly growing complex AccuProbe-positive organism was isolated from the sputum and pleural fluid of a 72-year-old female with bronchiectasis. The unusual morphology of the organism prompted further identification by 16S rRNA gene sequencing, revealing a perfect identity with previously uncharacterized strain sp. MCRO 8 (GenBank accession no. X93034), with the closest established species by 16S rDNA analysis being . HPLC of the organism corresponded to previously obtained patterns identified as -like and, upon sequence evaluation of a selection of strains with a similar profile, more were subsequently identified as MCRO 8. A total of 16 strains isolated from human respiratory samples were evaluated in the characterization of this novel species, for which the name sp. nov. is proposed. The type strain is strain 00-250 (=ATCC BAA-544=DSM 44616=CIP 108114).

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2004-05-01
2024-04-18
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References

  1. Brown B. A., Wallace R. J. Jr, Onyi G. O. 1992; Activities of clarithromycin against eight slowly growing species of nontuberculous mycobacteria, determined by using a broth microdilution MIC system. Antimicrob Agents Chemother 36:1987–1990 [CrossRef]
    [Google Scholar]
  2. Brunello F., Ligozzi M., Cristelli E., Bonora S., Tortoli E., Fontana R. 2001; Identification of 54 mycobacterial species by PCR-restriction fragment length polymorphism analysis of the hsp65 gene. J Clin Microbiol 39:2799–2806 [CrossRef]
    [Google Scholar]
  3. Butler W. R., Guthertz L. S. 2001; Mycolic acid analysis by high-performance liquid chromatography for identification of Mycobacterium species. Clin Microbiol Rev 14:704–726 [CrossRef]
    [Google Scholar]
  4. Butler W. R., Floyd M. M., Silcox V. A. & 9 other authors 1996 Standardized Method for HPLC Identification of Mycobacteria Atlanta: US Dept of Health and Human Services, Centers for Disease Control and Prevention;
    [Google Scholar]
  5. Cregan P., Yajko D. M., Ng V. L., Gonzalez P. C., Nassos P. S., Sanders C. A., Hadley W. K. 1992; Use of DNA probes to detect Mycobacterium intracellulare and “X” mycobacteria among clinical isolates of Mycobacterium avium complex. J Infect Dis 166:191–194 [CrossRef]
    [Google Scholar]
  6. da Silva Rocha A., Werneck Barreto A. M., Dias Campos C. E., Villas-Boas da Silva M., Fonseca L., Saad M. H., Degrave W. M., Suffys P. N. 2002; Novel allelic variants of mycobacteria isolated in Brazil as determined by PCR-restriction enzyme analysis of hsp65 . J Clin Microbiol 40:4191–4196 [CrossRef]
    [Google Scholar]
  7. Frothingham R., Wilson K. H. 1993; Sequence-based differentiation of strains in the Mycobacterium avium complex. J Bacteriol 175:2818–2825
    [Google Scholar]
  8. Harmsen D., Rothganger J., Frosch M., Albert J. 2002; RIDOM: ribosomal differentiation of medical micro-organisms database. Nucleic Acids Res 30:416–417 [CrossRef]
    [Google Scholar]
  9. Heifets L., Lindholm-Levy P., Libonati J. P., Hooper N., Laszlo A., Cynamon M., Siddiqi S. 1993 Radiometric Broth Macrodilution Method for the Determination of Minimal Inhibitory Concentrations (MIC) with Mycobacterium avium Complex Isolates Denver: National Jewish Center for Immunology and Respiratory Medicine;
    [Google Scholar]
  10. Kent P. T., Kubica G. P. 1985 Public Health Mycobacteriology: a Guide for the Level III Laboratory Atlanta: US Department of Health and Human Services, Centers for Disease Control;
    [Google Scholar]
  11. Leao S. C., Briones M. R., Sircili M. P., Balian S. C., Mores N., Ferreira-Neto J. S. 1999; Identification of two novel Mycobacterium avium allelic variants in pig and human isolates from Brazil by PCR-restriction enzyme analysis. J Clin Microbiol 37:2592–2597
    [Google Scholar]
  12. Lumb R., Goodwin A., Ratcliff R., Stapledon R., Holland A., Bastian I. 1997; Phenotypic and molecular characterization of three clinical isolates of Mycobacterium interjectum . J Clin Microbiol 35:2782–2785
    [Google Scholar]
  13. NCCLS 2000 Susceptibility testing of mycobacteria, nocardia, and other aerobic actinomycetes . Tentative standard M24-T2, 2nd edn. Wayne, PA: National Committee for Laboratory Standards;
    [Google Scholar]
  14. Rogall T., Wolters J., Flohr T., Böttger E. C. 1990; Towards a phylogeny and definition of species at the molecular level within the genus Mycobacterium . Int J Syst Bacteriol 40:323–330 [CrossRef]
    [Google Scholar]
  15. Shinnick T. M. 1987; The 65-kilodalton antigen of Mycobacterium tuberculosis . J Bacteriol 169:1080–1088
    [Google Scholar]
  16. Smole S. C., McAleese F., Ngampasutadol J., von Reyn C. F., Arbeit R. D. 2002; Clinical and epidemiological correlates of genotypes within the Mycobacterium avium complex defined by restriction and sequence analysis of hsp65 . J Clin Microbiol 40:3374–3380 [CrossRef]
    [Google Scholar]
  17. Soini H., Bottger E. C., Viljanen M. K. 1994; Identification of mycobacteria by PCR-based sequence determination of the 32-kilodalton protein gene. J Clin Microbiol 32:2944–2947
    [Google Scholar]
  18. Soini H., Eerola E., Viljanen M. K. 1996; Genetic diversity among Mycobacterium avium complex AccuProbe-positive isolates. J Clin Microbiol 34:55–57
    [Google Scholar]
  19. Springer B., Kirschner P., Rost-Meyer G., Schroder K. H., Kroppenstedt R. M., Bottger E. C. 1993; Mycobacterium interjectum , a new species isolated from a patient with chronic lymphadenitis. J Clin Microbiol 31:3083–3089
    [Google Scholar]
  20. Springer B., Stockman L., Teschner K., Roberts G. D., Bottger E. C. 1996; Two-laboratory collaborative study on identification of mycobacteria: molecular versus phenotypic methods. J Clin Microbiol 34:296–303
    [Google Scholar]
  21. Strässle A., Truong M., Pfyffer G. E. 2001; Identification of non-tuberculous mycobacteria by PCR/restriction enzyme analysis of the 65-kDa heat shock protein gene: trick or treat?. In Abstracts of the 102nd General Meeting of the American Society for Microbiology , abstract U11 Washington, DC: American Society for Microbiology;
    [Google Scholar]
  22. Taylor T. B., Patterson C., Hale Y., Safranek W. W. 1997; Routine use of PCR-restriction fragment length polymorphism analysis for identification of mycobacteria growing in liquid media. J Clin Microbiol 35:79–85
    [Google Scholar]
  23. Telenti A., Marchesi F., Balz M., Bally F., Bottger E. C., Bodmer T. 1993; Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J Clin Microbiol 31:175–178
    [Google Scholar]
  24. Torkko P., Suomalainen S., Iivanainen E., Tortoli E., Suutari M., Seppänen J., Paulin L., Katila M.-L. 2002; Mycobacterium palustre sp. nov., a potentially pathogenic, slowly growing mycobacterium isolated from clinical and veterinary specimens and from Finnish stream waters. Int J Syst Evol Microbiol 52:1519–1525 [CrossRef]
    [Google Scholar]
  25. Tortoli E., Bartoloni A., Burrini C., Colombrita D., Mantella A., Pinsi G., Simonetti M. T., Swierczynski G., Bottger E. C. 1996; Characterization of an isolate of the newly described species Mycobacterium interjectum . Zentbl Bakteriol 283:286–294 [CrossRef]
    [Google Scholar]
  26. Tortoli E., Piersimoni C., Kirschner P. 10 other authors 1997; Characterization of mycobacterial isolates phylogenetically related to, but different from Mycobacterium simiae . J Clin Microbiol 35:697–702
    [Google Scholar]
  27. Turenne C. Y., Tschetter L., Wolfe J., Kabani A. 2001; Necessity of quality-controlled 16S rRNA gene sequence databases: identifying nontuberculous Mycobacterium species. J Clin Microbiol 39:3637–3648 [CrossRef]
    [Google Scholar]
  28. Turenne C., Chedore P., Wolfe J., Jamieson F., Broukhanski G., May K., Kabani A. 2002; Mycobacterium lacus sp. nov., a novel slowly growing, non-chromogenic clinical isolate. Int J Syst Evol Microbiol 52:2135–2140 [CrossRef]
    [Google Scholar]
  29. Viljanen M. K., Olkkonen L., Katila M. L. 1993; Conventional identification characteristics, mycolate and fatty acid composition, and clinical significance of MAIX AccuProbe-positive isolates of Mycobacterium avium complex. J Clin Microbiol 31:1376–1378
    [Google Scholar]
  30. Wallace R. J. Jr, Nash D. R., Steele L. C., Steingrube V. 1986; Susceptibility testing of slowly growing mycobacteria by a microdilution MIC method with 7H9 broth. J Clin Microbiol 24:976–981
    [Google Scholar]
  31. Wallace R. J., Glassroth J. Jr, Griffith D. E., Olivier K. N., Cook J. L., Gordin F. 1997; Diagnosis and treatment of disease caused by nontuberculous mycobacteria. Official statement of the American Thoracic Society. Am J Respir Crit Care Med 156:S1–S25 [CrossRef]
    [Google Scholar]
  32. Wallace R. J. Jr, Brown-Elliott B. A., Ward S. C., Crist C. J., Mann L. B., Wilson R. W. 2001; Activities of linezolid against rapidly growing mycobacteria. Antimicrob Agents Chemother 45:764–767 [CrossRef]
    [Google Scholar]
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