1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 61, Issue 10

gen. nov., sp. nov., isolated from seawater Free

Abstract

A yellow-coloured, rod-shaped, Gram-reaction-negative and aerobic bacterial strain, designated JC2675, was isolated from a seawater sample from Jeju Island, Korea. The isolate required sea salts for growth. Gliding motility was observed. Flexirubin-type pigments were absent. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain JC2675 represented a distinct phyletic line that reflected a novel generic status within the family with relatively low gene sequence similarities (<95.7 %) to other recognized genera. The predominant isoprenoid quinone (MK-6) and DNA G+C content (30 mol%) were consistent with the assignment of the novel strain to the family , but overall phenotypic traits demonstrated that the novel strain was not closely affiliated with any previously described genus. Based on data from a study using a polyphasic taxonomic approach, it is proposed that strain JC2675 represents a new genus and novel species belonging to the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is JC2675 ( = KACC 14153 = JCM 17107).

  • Published Online:
Funding
This study was supported by the:
  • Priority Research Centers Program (Award 2 00 8- 00 5 J 00 201)
  • National Research Foundation (Award 2010-0017955)
  • Korean government (MEST),
  • project on survey and excavation of Korean indigenous species of the National Institute of Biological Resources(NIBR) under the Ministry of Environment
  • Regional SubGenBank Support Program of Rural Development Administration, Republic of Korea
© 2011 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.027441-0
2011-10-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/10/2475.html?itemId=/content/journal/ijsem/10.1099/ijs.0.027441-0&mimeType=html&fmt=ahah

References

  1. Bernardet J. F., Nakagawa Y. 2006; An introduction to the family Flavobacteriaceae . In The Prokaryotes pp. 455–480 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E. New York: Springer; [CrossRef]
     [Google Scholar]
  2. Bernardet J. F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [View Article][PubMed]
     [Google Scholar]
  3. Bowman J. P. 2000; Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50:1861–1868[PubMed]
     [Google Scholar]
  4. Bowman J. P., Nichols D. S. 2005; Novel members of the family Flavobacteriaceae from Antarctic maritime habitats including Subsaximicrobium wynnwilliamsii gen. nov., sp. nov., Subsaximicrobium saxinquilinus sp. nov., Subsaxibacter broadyi gen. nov., sp. nov., Lacinutrix copepodicola gen. nov., sp. nov., and novel species of the genera Bizionia, Gelidibacter and Gillisia . Int J Syst Evol Microbiol 55:1471–1486 [View Article][PubMed]
     [Google Scholar]
  5. Chun J., Goodfellow M. 1995; A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45:240–245 [View Article][PubMed]
     [Google Scholar]
  6. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef]
     [Google Scholar]
  7. Collins M. D. 1985; Analysis of isoprenoid quinones. Methods Microbiol 18:329–366 [View Article]
     [Google Scholar]
  8. Einen J., Ovreås L. 2006; Flaviramulus basaltis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from seafloor basalt. Int J Syst Evol Microbiol 56:2455–2461 [View Article][PubMed]
     [Google Scholar]
  9. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
     [Google Scholar]
  10. Felsenstein J. 1993; phylip (phylogenetic inference package) version 3.5.1. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA
  11. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. 1974; Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24:54–63 [View Article]
     [Google Scholar]
  12. Jeon Y. S., Chung H., Park S., Hur I., Lee J. H., Chun J. 2005; jphydit: a java-based integrated environment for molecular phylogeny of ribosomal RNA sequences. Bioinformatics 21:3171–3173 [View Article][PubMed]
     [Google Scholar]
  13. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol. 3 pp. 21–132 Edited by Munro H. N. New York: Academic Press;
     [Google Scholar]
  14. Lee D. H., Kahng H. Y., Lee Y. S., Jung J. S., Kim J. M., Chung B. S., Park S. K., Jeon C. O. 2009; Jejuia pallidilutea gen. nov., sp. nov., a new member of the family Flavobacteriaceae isolated from seawater. Int J Syst Evol Microbiol 59:2148–2152 [View Article][PubMed]
     [Google Scholar]
  15. Lee S. Y., Park S., Oh T. K., Yoon J. H. 2010; Description of Olleya aquimaris sp. nov., isolated from seawater, and emended description of the genus Olleya Mancuso Nichols et al. 2005. Int J Syst Evol Microbiol 60:887–891 [View Article][PubMed]
     [Google Scholar]
  16. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [View Article][PubMed]
     [Google Scholar]
  17. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
     [Google Scholar]
  18. Nedashkovskaya O. I., Kim S. B., Han S. K., Rhee M. S., Lysenko A. M., Rohde M., Zhukova N. V., Frolova G. M., Mikhailov V. V., Bae K. S. 2004; Algibacter lectus gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from green algae. Int J Syst Evol Microbiol 54:1257–1261 [View Article][PubMed]
     [Google Scholar]
  19. Nedashkovskaya O. I., Vancanneyt M., Kim S. B., Hoste B., Bae K. S. 2007; Algibacter mikhailovii sp. nov., a novel marine bacterium of the family Flavobacteriaceae, and emended description of the genus Algibacter . Int J Syst Evol Microbiol 57:2147–2150 [View Article][PubMed]
     [Google Scholar]
  20. Nedashkovskaya O. I., Kwon K. K., Yang S. H., Lee H. S., Chung K. H., Kim S. J. 2008; Lacinutrix algicola sp. nov. and Lacinutrix mariniflava sp. nov., two novel marine alga-associated bacteria and emended description of the genus Lacinutrix . Int J Syst Evol Microbiol 58:2694–2698 [View Article][PubMed]
     [Google Scholar]
  21. Nichols C. M., Bowman J. P., Guezennec J. 2005; Olleya marilimosa gen. nov., sp. nov., an exopolysaccharide-producing marine bacterium from the family Flavobacteriaceae, isolated from the Southern Ocean. Int J Syst Evol Microbiol 55:1557–1561 [View Article][PubMed]
     [Google Scholar]
  22. Ronquist F., Huelsenbeck J. P. 2003; MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574 [View Article][PubMed]
     [Google Scholar]
  23. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
     [Google Scholar]
  24. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  25. Swofford D. L. 1998; Phylogenetic analysis using parsimony (paup). Version 4. Sunderland, MA: Sinauer Associates;
  26. Weeks O. B. 1981; Preliminary studies of the pigments of Flavobacterium breve NCTC 11099 and Flavobacterium odoratum NCTC 11036. In The Flavobacterium-Cytophaga Group pp. 108–114 Edited by Reichenbach H., Weeks O. B. Weinheim: Gesellschaft fur Biotechnologische Forschung;
     [Google Scholar]
  27. Woese C. R., Magrum L. J., Gupta R., Siegel R. B., Stahl D. A., Kop J., Crawford N., Brosius J., Gutell R. et al. other authors 1980; Secondary structure model for bacterial 16S ribosomal RNA: phylogenetic, enzymatic and chemical evidence. Nucleic Acids Res 8:2275–2293 [View Article][PubMed]
     [Google Scholar]
  28. Yoon B. J., Lee D. H., Kang B. J., Kahng H. Y., Oh Y. S., Sohn J. H., Choi E. S., Oh D. C. 2010; Hyunsoonleella jejuensis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from seawater. Int J Syst Evol Microbiol 60:382–386 [View Article][PubMed]
     [Google Scholar]
  29. ZoBell C. E. 1941; Studies on marine bacteria. I. The cultural requirements of heterotrophic aerobes. J Mar Res 4:42–75
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.027441-0
Loading
Pontirhabdus pectinivorans gen. nov., sp. nov., isolated from seawater
Int J Syst Evol Microbiol 61, 2475 (2011); https://doi.org/10.1099/ijs.0.027441-0
/content/journal/ijsem/10.1099/ijs.0.027441-0
/content/journal/ijsem/10.1099/ijs.0.027441-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error