1887

Abstract

A novel moderately thermophilic, microaerobic to anaerobic, chemolithoautotrophic bacterium, designated strain CR, was isolated from a deep-sea hydrothermal vent site at 36°N on the Mid-Atlantic Ridge. Cells were Gram-negative, non-motile rods. The organism grew at 45–65 °C and pH 6·5–7·4, with optimum growth at 55 °C and pH 6·9–7·1. The NaCl range for growth was 5–50 g l (optimum 30 g l). Strain CR was an obligate chemolithoautotroph, growing with H as energy source, sulfur, nitrate or oxygen as electron acceptors and CO as carbon source. Hydrogen sulfide and ammonium were the respective products of sulfur and nitrate reduction. The G+C content of the genomic DNA was 32·1 mol%. Based on 16S rRNA gene sequence analysis, this organism was most closely related to (94·9 % similarity). On the basis of phenotypic and phylogenetic data, it is proposed that the isolate represents a novel species, sp. nov. The type strain is CR (=DSM 15016=JCM 11957). The phylogenetic data also correlate well with the significant phenotypic differences between the lineage encompassing the genera and and other members of the class ‘’. The lineage encompassing the genera and is therefore proposed as a new order, ord. nov., represented by a single family, fam. nov.

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2004-01-01
2024-03-29
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