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Volume 61, Issue 12

gen. nov., sp. nov., a peritrichously flagellated, chain-forming, agar-hydrolysing gammaproteobacterium from seawater Free

Abstract

A novel Gram-negative, strictly aerobic, agar-hydrolysing bacterium, designated YM01, was isolated from seawater samples collected from the Yellow Sea (coastal region of Qingdao, PR China). Cells were rod-shaped, peritrichously flagellated and formed long chains end-to-end. The isolate had an absolute requirement for Na ions, but not seawater, for growth and grew optimally at about 28 °C, in 2 % NaCl and at pH 8.0–9.0. The isolate could not be cultured in marine broth 2216, but grew well on marine agar 2216. YM01 was able to hydrolyse cellulose, starch, aesculin and Tween 80, but not egg yolk, gelatin, urea or casein. 16S rRNA gene sequence analysis demonstrated that this isolate was unique, showing only 88.4–91.0 % sequence similarity to its closest neighbours, including members of the genera (88.4–91.0 %), (88.7–89.6 %), (89.3–90.4 %), (89.0 %), (90.1–90.3 %) and (88.5–89.9 %). Phylogenetic analyses demonstrated that strain YM01 formed a distinct clade closely related to species of the family within the group of -like gammaproteobacteria. It contained menaquinone MK-7 as the predominant isoprenoid quinone and C (38.3 %), Cω7 and/or iso-C 2-OH (29.0 %), Cω7 (9.3 %) and C 3-OH (8.2 %) as major cellular fatty acids. Phosphatidylethanolamine, phosphatidylglycerol and an aminophospholipid were the major phospholipid constituents. The DNA G+C content was 44.8 mol%. Based on its phenotypic, chemotaxonomic and phylogenetic distinctiveness, strain YM01 is considered to represent a novel species in a new genus in the , for which the name gen. nov., sp. nov. is proposed; the type strain of is YM01 ( = CGMCC 1.10245  = DSM 23111  = JCM 16580).

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Funding
This study was supported by the:
  • National High Technology R&D Program of China (Award 2007AA09Z434)
  • National Natural Science Foundation of China (Award 40876067)
© 2011 IUMS
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2011-12-01
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Catenovulum agarivorans gen. nov., sp. nov., a peritrichously flagellated, chain-forming, agar-hydrolysing gammaproteobacterium from seawater
Int J Syst Evol Microbiol 61, 2866 (2011); https://doi.org/10.1099/ijs.0.027565-0
/content/journal/ijsem/10.1099/ijs.0.027565-0
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