%0 Journal Article %A Seiler, Herbert %A Schmidt, Verena %A Wenning, Mareike %A Scherer, Siegfried %T Bacillus kochii sp. nov., isolated from foods and a pharmaceuticals manufacturing site %D 2012 %J International Journal of Systematic and Evolutionary Microbiology, %V 62 %N Pt_5 %P 1092-1097 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.027771-0 %I Microbiology Society, %X Three Gram-staining-positive, strictly aerobic, motile, catalase-positive, endospore-forming rods, designated WCC 4582T, WCC 4581 and WCC 4583, were isolated from two different food sources and a pharmaceuticals production site. The three isolates were highly similar in their 16S rRNA gene sequences (100 % similarity) and groEL sequences (99.2–100 % similarity), Fourier-transform infrared spectroscopic fingerprints and other features tested. The isolates were most closely related to Bacillus horneckiae ; the isolates and the type strain of B. horneckiae shared 97.6 % and 89.6 % 16S rRNA gene and groEL sequence similarities, respectively. The organisms grew optimally at 30 °C, at pH 7 and in the presence of 0.5 % (w/v) NaCl. The cell-wall peptidoglycan of WCC 4582T contained meso-diaminopimelic acid (A1γ) and the genomic DNA G+C content was 36.4 mol%. DNA–DNA relatedness between strain WCC 4582T and B. horneckiae NRRL B-59162T was 17 %. The three isolates are considered to represent a novel species of the genus Bacillus , for which the name Bacillus kochii sp. nov. is proposed. The type strain is WCC 4582T ( = DSM 23667T = CCUG 59877T = LMG 25855T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.027771-0