1887

Abstract

A Gram-negative, non-motile, non-sporulating bacterial strain, designated LYX05, was isolated from coastal sediment of Qingdao, China, on the coast of the Yellow Sea. Strain LYX05 was aerobic and heterotrophic. The strain grew optimally at 37 °C and pH 7.5 and in the presence of 2 % (w/v) NaCl. Colonies were 1–2 mm in diameter, circular, reddish orange and shiny with entire edges on marine agar medium. Cells were rods (0.3–0.5 µm wide and 0.8–1.6 µm long). The dominant fatty acids were iso-C (40.82 %) and C (10.45 %). The DNA G+C content was 42.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LYX05 was phylogenetically related to the members of the genus and the closest relative was 7-UAH (95.8 % 16S rRNA gene sequence similarity). On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain LYX05 was considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LYX05 ( = JCM 16561  = DSM 23095  = LMG 25474).

Funding
This study was supported by the:
  • Development Project Programs of Shandong (Award 2008GG10007003)
  • National High Technology R&D Program of China (Award 2007AA09Z434)
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2011-12-01
2024-03-29
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References

  1. Ahmed I., Yokota A., Fujiwara T. 2007; Chimaereicella boritolerans sp. nov., a boron-tolerant and alkaliphilic bacterium of the family Flavobacteriaceae isolated from soil. Int J Syst Evol Microbiol 57:986–992 [View Article][PubMed]
    [Google Scholar]
  2. Bowman J. P., Nichols C. M., Gibson J. A. 2003; Algoriphagus ratkowskyi gen. nov., sp. nov., Brumimicrobium glaciale gen. nov., sp. nov., Cryomorpha ignava gen. nov., sp. nov. and Crocinitomix catalasitica gen. nov., sp. nov., novel flavobacteria isolated from various polar habitats. Int J Syst Evol Microbiol 53:1343–1355 [View Article][PubMed]
    [Google Scholar]
  3. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y.-W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  4. Copa-Patiño J. L., Arenas M., Soliveri J., Sánchez-Porro C., Ventosa A. 2008; Algoriphagus hitonicola sp. nov., isolated from an athalassohaline lagoon. Int J Syst Evol Microbiol 58:424–428 [View Article][PubMed]
    [Google Scholar]
  5. Liu Y., Li H., Jiang J.-T., Liu Y.-H., Song X.-F., Xu C.-J., Liu Z.-P. 2009; Algoriphagus aquatilis sp. nov., isolated from a freshwater lake. Int J Syst Evol Microbiol 59:1759–1763 [View Article][PubMed]
    [Google Scholar]
  6. Mesbah M., Whitman W. B. 1989; Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr A 479:297–306 [View Article][PubMed]
    [Google Scholar]
  7. Nedashkovskaya O. I., Vancanneyt M., Van Trappen S., Vandemeulebroecke K., Lysenko A. M., Rohde M., Falsen E., Frolova G. M., Mikhailov V. V., Swings J. 2004; Description of Algoriphagus aquimarinus sp. nov., Algoriphagus chordae sp. nov. and Algoriphagus winogradskyi sp. nov., from sea water and algae, transfer of Hongiella halophila Yi and Chun 2004 to the genus Algoriphagus as Algoriphagus halophilus comb. nov. and emended descriptions of the genera Algoriphagus Bowman et al. 2003 and Hongiella Yi and Chun 2004. Int J Syst Evol Microbiol 54:1757–1764 [View Article][PubMed]
    [Google Scholar]
  8. Nedashkovskaya O. I., Kim S. B., Kwon K. K., Shin D. S., Luo X., Kim S.-J., Mikhailov V. V. 2007; Proposal of Algoriphagus vanfongensis sp. nov., transfer of members of the genera Hongiella Yi and Chun 2004 emend. Nedashkovskaya et al. 2004 and Chimaereicella Tiago et al. 2006 to the genus Algoriphagus, and emended description of the genus Algoriphagus Bowman et al. 2003 emend. Nedashkovskaya et al. 2004. Int J Syst Evol Microbiol 57:1988–1994 [View Article][PubMed]
    [Google Scholar]
  9. Niemann S., Pühler A., Tichy H. V., Simon R., Selbitschka W. 1997; Evaluation of the resolving power of three different DNA fingerprinting methods to discriminate among isolates of a natural Rhizobium meliloti population. J Appl Microbiol 82:477–484 [View Article][PubMed]
    [Google Scholar]
  10. Park S., Kang S.-J., Oh K.-H., Oh T.-K., Yoon J.-H. 2010; Algoriphagus lutimaris sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 60:200–204 [View Article][PubMed]
    [Google Scholar]
  11. Reichenbach H. 1989; Order I. Cytophagales Leadbetter 1974. In Bergey’s Manual of Systematic Bacteriology vol. 3 pp. 2011–2013 Edited by Staley J. T., Bryant M. P., Pfennig N., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  12. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  13. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  14. Tiago I., Mendes V., Pires C., Morais P. V., Veríssimo A. 2006; Chimaereicella alkaliphila gen. nov., sp. nov., a Gram-negative alkaliphilic bacterium isolated from a nonsaline alkaline groundwater. Syst Appl Microbiol 29:100–108 [View Article][PubMed]
    [Google Scholar]
  15. Tindall B. J., Sikorski J., Smibert R. M., Krieg N. R. 2007; Phenotypic characterization and the principles of comparative systematics. In Methods for General and Molecular Microbiology pp. 330–393 Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  16. Van Trappen S., Vandecandelaere I., Mergaert J., Swings J. 2004; Algoriphagus antarcticus sp. nov., a novel psychrophile from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 54:1969–1973 [View Article][PubMed]
    [Google Scholar]
  17. Xie C.-H., Yokota A. 2003; Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49:345–349 [View Article][PubMed]
    [Google Scholar]
  18. Yi H., Chun J. 2004; Hongiella mannitolivorans gen. nov., sp. nov., Hongiella halophila sp. nov. and Hongiella ornithinivorans sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 54:157–162 [View Article][PubMed]
    [Google Scholar]
  19. Yoon J.-H., Yeo S.-H., Oh T.-K. 2004; Hongiella marincola sp. nov., isolated from sea water of the East Sea in Korea. Int J Syst Evol Microbiol 54:1845–1848 [View Article][PubMed]
    [Google Scholar]
  20. Yoon J.-H., Lee M.-H., Kang S.-J., Oh T.-K. 2006; Algoriphagus terrigena sp. nov., isolated from soil. Int J Syst Evol Microbiol 56:777–780 [View Article][PubMed]
    [Google Scholar]
  21. Young C.-C., Lin S.-Y., Arun A. B., Shen F.-T., Chen W.-M., Rekha P. D., Langer S., Busse H.-J., Wu Y.-H., Kämpfer P. 2009; Algoriphagus olei sp. nov., isolated from oil-contaminated soil. Int J Syst Evol Microbiol 59:2909–2915 [View Article][PubMed]
    [Google Scholar]
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