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Volume 62, Issue 2

sp. nov., a marine bacterium isolated from sediment Free

Abstract

A protease-producing marine bacterium, designated CF12-14, was isolated from sediment of the South China Sea. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain CF12-14 formed a separate lineage within the genus (). The isolate showed the highest 16S rRNA gene sequence similarity with ISL-52 (94.7 %), CL-SP19 (94.6 %) and other members of the genus (91.9–94.6 %). Cells were Gram-negative, aerobic, flagellated, straight or slightly curved, and often formed buds and prosthecae. Strain CF12-14 grew at 4–42 °C (optimum 30–35 °C) and with 0.1–15 % (w/v) NaCl (optimum 2–3 %). The isolate reduced nitrate to nitrite and hydrolysed DNA, but did not produce acids from sugars. The predominant cellular fatty acids were iso-C (27.4 %), iso-C (16.0 %) and iso-Cω9 (15.8 %). The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The major respiratory quinone was ubiquinone 8. The DNA G+C content was 50.4 mol%. The phylogenetic, phenotypic and chemotaxonomic data supported the conclusion that CF12-14 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CF12-14 ( = CCTCC AB 208166 = KACC 13974).

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Funding
This study was supported by the:
  • Hi-Tech Research and Development Program of China (Award 2007AA021306 and 2007AA091903)
  • COMRA Program (Award DYXM-115-02-2-20 and DYXM-115-02-2-6)
  • National Natural Science Foundation of China (Award 40776032)
© 2012 IUMS
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2012-02-01
2024-04-19
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Idiomarina maris sp. nov., a marine bacterium isolated from sediment
Int J Syst Evol Microbiol 62, 370 (2012); https://doi.org/10.1099/ijs.0.027896-0
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