1887

Abstract

Two novel strains of marine lactic acid bacteria, isolated from decaying marine algae collected from a subtropical area of Japan, are described. The isolates, designated O24-2 and O25-2, were Gram-positive, non-sporulating and non-motile. They lacked catalase and quinones. Under anaerobic cultivation conditions, lactate was produced from glucose with the production of formate, acetate and ethanol in a molar ratio of approximately 2 : 1 : 1. Under aerobic cultivation conditions, acetate and lactate were produced from carbohydrates and related compounds. The isolates were slightly halophilic, highly halotolerant and alkaliphilic. They were able to grow in 0–17.0 % (w/v) NaCl, with optimum growth of strains O24-2 and O25-2 at 1.0–3.0 and 1.0–2.0 % (w/v) NaCl, respectively. Growth of strain O24-2 was observed at pH 7.5–9.5, with optimum growth at pH 8.0–8.5. Comparative 16S rRNA gene sequence analysis revealed that the isolates occupied a phylogenetic position within the genus , showing highest similarity (99.6 %) to T129-2-1. Although sequence similarity was high, the DNA–DNA relatedness value between strain O24-2 and . T129-2-1 was 27 %, indicating that they are members of distinct species. The DNA G+C contents of O24-2 and O25-2 were 43.7 and 44.4 mol%, respectively, and DNA–DNA relatedness between the isolates was 89 %. The cell-wall peptidoglycan was type A4β, Orn--Asp. The major cellular fatty acid components were C, C and Cω9. Based on phenotypic characteristics and genetic distinctiveness, the isolates were classified as representatives of a novel species within the genus , for which the name sp. nov. is proposed; the type strain is O24-2 ( = DSM 23664 = NBRC 107172).

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2011-12-01
2024-03-29
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