1887

Abstract

A pink-pigmented, aerobic, facultatively methylotrophic bacterium, strain BJ001, was isolated from internal poplar tissues (× DN34) and identified as a member of the genus . Phylogenetic analyses showed that strain BJ001 is related to , , and . However, strain BJ001 differed from these species in its carbon-source utilization pattern, particularly its use of methane as the sole source of carbon and energy, an ability that is shared with only one other member of the genus, . In addition, strain BJ001 is the only member of the genus to be described as an endophyte of poplar trees. On the basis of its physiological, genotypic and ecological properties, the isolate is proposed as a member of a novel species of the genus , sp. nov. (type strain, BJ001=ATCC BAA-705=NCIMB 13946).

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2004-07-01
2024-03-28
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References

  1. Austin B., Goodfellow M. 1979; Pseudomonas mesophilica , a new species of pink bacteria isolated from leaf surfaces. Int J Syst Bacteriol 29:373–378 [CrossRef]
    [Google Scholar]
  2. Austin B., Goodfellow M., Dickinson C. H. 1978; Numerical taxonomy of phylloplane bacteria isolated from Lolium perenne . J Gen Microbiol 104:139–155 [CrossRef]
    [Google Scholar]
  3. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. 1999 Current Protocols in Molecular Biology , 4th edn. New York: Wiley;
    [Google Scholar]
  4. Bligh E. G., Dyer W. J. 1959; A rapid method of total lipid extraction and purification. Can J Med Sci 37:911–917
    [Google Scholar]
  5. Bousfield I. J., Green P. N. 1985; Reclassification of bacteria of the genus Protomonas Urakami and Komagata 1984 in the genus Methylobacterium (Patt, Cole, and Hanson) emend. Green and Bousfield 1983 Int J Syst Bacteriol 35:209 [CrossRef]
    [Google Scholar]
  6. Bozzola J. J., Russell L. D. 1998 Electron Microscopy , 2nd edn. Sudbury, MS: Jones & Bartlett;
    [Google Scholar]
  7. Corpe W. A., Rheem S. 1989; Ecology of the methylotrophic bacteria on living leaf surfaces. FEMS Microbiol Ecol 62:243–250 [CrossRef]
    [Google Scholar]
  8. Denhardt D. T. 1966; A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun 23:641–646 [CrossRef]
    [Google Scholar]
  9. de Zwart J. M. M., Nelisse P. N., Kuenen J. G. 1996; Isolation and characterization of Methylophaga sulfidovorans sp. nov.: an obligately methylotrophic, aerobic, dimethylsulfide oxidizing bacterium from a microbial mat. FEMS Microbiol Ecol 20:261–270
    [Google Scholar]
  10. Doronina N. V., Trotsenko Y. A., Tourova T. P., Kuznetsov B. B., Leisinger T. 2000; Methylopila helvetica sp. nov. and Methylobacterium dichloromethanicum sp. nov. – novel aerobic facultatively methylotrophic bacteria utilizing dichloromethane. Syst Appl Microbiol 23:210–218 [CrossRef]
    [Google Scholar]
  11. Doronina N. V., Trotsenko Y. A., Kuznetsov B. B., Tourova T. P., Salkinoja-Salonen M. S. 2002; Methylobacterium suomiense sp. nov. and Methylobacterium lusitanum sp. nov., aerobic, pink-pigmented, facultatively methylotrophic bacteria. Int J Syst Evol Microbiol 52:773–776 [CrossRef]
    [Google Scholar]
  12. Gerhardt P. R., Murray R. G. E., Wood W. A., Krieg N. R. (editors) 1994 Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  13. Goodwin K. D., Varner R. K., Crill P. M., Oremland R. S. 2001; Consumption of tropospheric levels of methyl bromide by C1 compound-utilizing bacteria and comparison to saturation kinetics. Appl Environ Microbiol 67:5437–5443 [CrossRef]
    [Google Scholar]
  14. Green P. N. 1992; The genus Methylobacterium . In The Prokaryotes , 2nd edn. pp  2342–2349 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H. New York: Springer;
    [Google Scholar]
  15. Green P. N., Bousfield I. J. 1982; A taxonomic study of some Gram-negative facultatively methylotrophic bacteria. J Gen Microbiol 128:623–638
    [Google Scholar]
  16. Green P. N., Bousfield I. J. 1983; Emendation of Methylobacterium Patt, Cole, and Hanson 1976; Methylobacterium rhodinum (Heumann 1962) comb. nov. corrig.; Methylobacterium radiotolerans (Ito and Iizuka 1971) comb. nov. corrig.; and Methylobacterium mesophilicum (Austin and Goodfellow 1979) comb. nov. Int J Syst Bacteriol 33:875–877 [CrossRef]
    [Google Scholar]
  17. Green P. N., Bousfield I. J., Hood D. 1988; Three new Methylobacterium species: M. rhodesianum sp. nov., M. zatmanii sp. nov., and M. fujisawaense sp. nov. Int J Syst Bacteriol 38:124–127 [CrossRef]
    [Google Scholar]
  18. Heumann W. 1962; Die Methodik der Kreuzung sternbildender Bakterien. Biol Zentbl 81:341–354 (in German
    [Google Scholar]
  19. Hiraishi A., Furuhata K., Matsumoto A., Koike K. A., Fukuyama M., Tabuchi K. 1995; Phenotypic and genetic diversity of chlorine-resistant Methylobacterium strains isolated from various environments. Appl Environ Microbiol 61:2099–2107
    [Google Scholar]
  20. Holland M. A., Polacco J. C. 1994; PPFMs and other covert contaminants: is there more to plant physiology than just plant?. Annu Rev Plant Physiol Plant Mol Biol 45:197–209 [CrossRef]
    [Google Scholar]
  21. Hornei B., Lüneberg E., Schmidt-Rotte H., Maaß M., Weber K., Heits F., Frosch M., Solbach W. 1999; Systemic infection of an immunocompromised patient with Methylobacterium zatmanii . J Clin Microbiol 37:248–250
    [Google Scholar]
  22. Hurek T., Wagner B., Reinhold-Hurek B. 1997; Identification of N2-fixing plant- and fungus-associated Azoarcus species by PCR-based genomic fingerprints. Appl Environ Microbiol 63:4331–4339
    [Google Scholar]
  23. Ito H., Iizuka H. 1971; Taxonomic studies on a radio-resistant Pseudomonas . XII. Studies on the microorganisms of cereal grain. Agric Biol Chem 35:1566–1571 [CrossRef]
    [Google Scholar]
  24. Ivanova E. G., Doronina N. V., Trotsenko Y. A. 2001; Aerobic methylobacteria are capable of synthesizing auxins. Microbiologiya 70:452–458 (in Russian
    [Google Scholar]
  25. Koenig R. L., Morris R. O., Polacco J. C. 2002; tRNA is the source of low-level trans -zeatin production in Methylobacterium spp. J Bacteriol 184:1832–1842 [CrossRef]
    [Google Scholar]
  26. Kouno K., Ozaki A. 1975; Distribution of methanol-utilizing bacteria. In Proceedings of the International Symposium on Microbial Growth on C1 Compounds pp  11–21 Osaka, Japan: Society of Fermentation Technology;
    [Google Scholar]
  27. Lidstrom M. E., Chistoserdova L. 2002; Plants in the pink: cytokinin production by Methylobacterium . J Bacteriol 184:1818 [CrossRef]
    [Google Scholar]
  28. McDonald I. R., Doronina N. V., Trotsenko Y. A., McAnulla C., Murrell J. C. 2001; Hyphomicrobium chloromethanicum sp. nov. and Methylobacterium chloromethanicum sp. nov., chloromethane-utilizing bacteria isolated from a polluted environment. Int J Syst Evol Microbiol 51:119–122
    [Google Scholar]
  29. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  30. Mo K., Lora C. O., Wanken A. E., Javanmardian M., Yang X., Kulpa C. F. 1997; Biodegradation of methyl t -butyl ether by pure bacterial cultures. Appl Microbiol Biotechnol 47:69–72 [CrossRef]
    [Google Scholar]
  31. Patt T. E., Cole G. C., Hanson R. S. 1976; Methylobacterium , a new genus of facultatively methylotrophic bacteria. Int J Syst Bacteriol 26:226–229 [CrossRef]
    [Google Scholar]
  32. Rock J. S., Goldberg I., Ben-Bassat A., Mateles R. I. 1976; Isolation and characterization of two methanol-utilizing bacteria. Agric Biol Chem 40:2129–2135 [CrossRef]
    [Google Scholar]
  33. Sambrook J., Russell D. 2000 Molecular Cloning: a Laboratory Manual , 3rd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  34. Schaefer J. K., Oremland R. S. 1999; Oxidation of methyl halides by the facultative methylotroph strain IMB-1. Appl Environ Microbiol 65:5035–5041
    [Google Scholar]
  35. Sy A., Giraud E., Jourand P. 8 other authors 2001; Methylotrophic Methylobacterium bacteria nodulate and fix nitrogen in symbiosis with legumes. J Bacteriol 183:214–220 [CrossRef]
    [Google Scholar]
  36. Tan Z., Hurek T., Vinuesa P., Muller P., Ladha J. K., Reinhold-Hurek B. 2001; Specific detection of Bradyrhizobium and Rhizobium strains colonizing rice ( Oryza sativa ) roots by 16S-23S ribosomal DNA intergenic spacer-targeted PCR. Appl Environ Microbiol 67:3655–3664 [CrossRef]
    [Google Scholar]
  37. Trotsenko Y. A., Ivanova E. G., Doronina N. V. 2001; Aerobic methylotrophic bacteria as phytosymbionts. Mikrobiologiya 70:725–736 (in Russian
    [Google Scholar]
  38. Truant A. L., Gulati R., Giger O., Satishchandran V., Caya J. G. 1998; Methylobacterium species: an increasingly important opportunistic pathogen. Lab Med 29:704–710
    [Google Scholar]
  39. Urakami T., Komagata K. 1984; Protomonas , Protomonas , a new genus of facultatively methylotrophic bacteria. Int J Syst Bacteriol 34:188–201 [CrossRef]
    [Google Scholar]
  40. Urakami T., Araki H., Suzuki K., Komagata K. 1993; Further studies of the genus Methylobacterium and description of Methylobacterium aminovorans sp. nov. Int J Syst Bacteriol 43:504–513 [CrossRef]
    [Google Scholar]
  41. Van Aken B., Schnoor J. L. 2002; Evidence of perchlorate () reduction in plant tissues (poplar tree) using radio-labeled [inline-graphic]. Environ Sci Technol 36:2783–2788 [CrossRef]
    [Google Scholar]
  42. Wood A. P., Kelly D. P., McDonald I. R., Jordan S. L., Morgan T. D., Khan S., Murrell J. C., Borodina E. 1998; A novel pink-pigmented facultative methylotroph, Methylobacterium thiocyanatum sp. nov., capable of growth on thiocyanate or cyanate as sole nitrogen sources. Arch Microbiol 169:148–158 [CrossRef]
    [Google Scholar]
  43. Yoshimura F. 1982; Phylloplane bacteria in a pine forest. Can J Microbiol 28:580–592 [CrossRef]
    [Google Scholar]
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