@article{mbs:/content/journal/ijsem/10.1099/ijs.0.028274-0, author = "Geymonat, Estefanía and Ferrando, Lucía and Tarlera, Silvana E.", title = "Methylogaea oryzae gen. nov., sp. nov., a mesophilic methanotroph isolated from a rice paddy field", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2011", volume = "61", number = "11", pages = "2568-2572", doi = "https://doi.org/10.1099/ijs.0.028274-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.028274-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A novel methanotroph, designated strain E10T, was isolated from a rice paddy field in Uruguay. Strain E10T grew on methane and methanol as sole carbon and energy sources. Cells were Gram-negative, non-motile, non-pigmented, slightly curved rods showing type I intracytoplasmic membranes arranged in stacks. The strain was neutrophilic and mesophilic; optimum growth occurred at 30–35 °C with no growth above 37 °C. The strain possessed only a particulate methane monooxygenase (pmoA). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain was most closely related to the moderately thermophilic strains Methylocaldum szegediense OR2T (91.6 % sequence similarity) and Methylococcus capsulatus Bath (91.5 %). Comparative sequence analysis of pmoA genes also confirmed that strain E10T formed a new lineage among the genera Methylocaldum and Methylococcus with 89 and 84 % derived amino acid sequence identity to Methylococcus capsulatus Bath and Methylocaldum gracile VKM-14LT, respectively. The DNA G+C content was 63.1 mol% and the major cellular fatty acid was C16 : 0 (62.05 %). Thus, strain E10T ( = JCM 16910T  = DSM 23452T) represents the type strain of a novel species within a new genus, for which the name Methylogaea oryzae gen. nov., sp. nov. is proposed.", }