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Volume 54, Issue 3

sp. nov., sp. nov. and sp. nov., isolated from air in the Russian space laboratory Mir Free

Abstract

Four Gram-positive bacteria, strains A1-17B, A1-22, A1-3 and A1-8, isolated from the air in the Russian space laboratory Mir, were subjected to a polyphasic taxonomic study. Phylogenetic analysis of the bacteria based on their 16S rDNA sequence showed that they belong to the genera (A1-17B), (A1-22) and (A1-3 and A1-8). Morphological, physiological, chemotaxonomic and genomic characteristics supported the assignments of these strains to these genera, but they could not be classified as any existing species within each respective genus. 16S rDNA similarity values between strain A1-17B and its neighbours, genomovar II, , and , were respectively 99·8, 98·0, 96·4 and 95·4 %. Polyphasic taxonomic evidence indicated that strain A1-17B should be categorized together with the unofficially named genomovar II, but clearly differentiated them from the established species of the genus . Strain A1-22 formed a coherent cluster with , , and in 16S rDNA sequence analysis, but DNA–DNA relatedness values were only 45·5, 35·3, 18·9 and 21·9 %. Strains A1-3 and A1-8 shared 99·9 % 16S rDNA sequence similarity, and strain A1-3 showed the highest level of 16S rDNA similarity, 96·6 %, to . Contrasting biochemical characteristics were also identified. Finally, as a result of the polyphasic taxonomic study, three of the strains are proposed as type strains of novel species: sp. nov. (A1-17B=GTC 867=JCM 11412=DSM 14556), sp. nov. (A1-22=GTC 1041=JCM 11411=DSM 44587) and sp. nov. (A1-3=GTC 863=JCM 11414=DSM 14555).

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2004-05-01
2024-04-20
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Rothia aeria sp. nov., Rhodococcus baikonurensis sp. nov. and Arthrobacter russicus sp. nov., isolated from air in the Russian space laboratory Mir
Int J Syst Evol Microbiol 54, 827 (2004); https://doi.org/10.1099/ijs.0.02828-0
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