1887

Abstract

A Gram-positive, non-spore-forming bacterium (GW39-1573) was isolated from soil of the Spanish island of Tenerife. 16S rRNA gene sequence similarity studies showed that strain GW39-1573 belonged to the genus and was most closely related to (98·0 %), (97·3 %), (97·5 %), (97·2 %) and (97·0 %). Strain GW39-1573 could be distinguished from all other validly described species by sequence similarity values of less than 97 %. Chemotaxonomic data [major menaquinone: MK-8(H); major polar lipids: diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and an unknown glycolipid and an unknown phospholipid; major fatty acids: C, C 9 and 10 methyl C] and the presence of mycolic acids supported the affiliation of strain GW39-1573 to the genus . The results of DNA–DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain GW39-1573 from those related species that showed 16S rRNA gene sequence similarity values of greater than 97 %. Strain GW39-1573 merits species status, and the name is proposed with the type strain GW39-1573 (=DSM 44704=CIP 107929).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02841-0
2004-03-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/2/ijs540381.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02841-0&mimeType=html&fmt=ahah

References

  1. Albuquerque de Barros E. V. S., Manfio G. P., Ribiero Maitan V., Mendes Bataus L. A., Kim S. B., Maldonado L. A., Goodfellow M. 2003; Nocardia cerradoensis sp. nov., a novel isolate from Cerrado soil in Brazil. Int J Syst Evol Microbiol 53:29–33 [CrossRef]
    [Google Scholar]
  2. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. 1994 Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  3. Goodfellow M., Isik K., Yates E. 1999; Actinomycete systematics: an unfinished synthesis. Nova Acta Leopold 312:47–82
    [Google Scholar]
  4. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [CrossRef]
    [Google Scholar]
  5. Kämpfer P., Steiof M., Dott W. 1991; Microbiological characterisation of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21:227–251 [CrossRef]
    [Google Scholar]
  6. Kämpfer P., Dreyer U., Neef A., Dott W., Busse H.-J. 2003; Chryseobacterium defluvii sp. nov., isolated from wastewater. Int J Syst Evol Microbiol 53:93–97 [CrossRef]
    [Google Scholar]
  7. Kumar S., Tamura K., Jakobsen I. B., Nei M. 2001; mega2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245 [CrossRef]
    [Google Scholar]
  8. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. 1977; Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117 [CrossRef]
    [Google Scholar]
  9. Minnikin D. E., Iwona G., Hutchinson G., Caldicott A. B. 1980; Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188:221–233 [CrossRef]
    [Google Scholar]
  10. Strunk O., Gross O., Reichel B. 10 other authors 2000 arb: a software environment for sequence data Department of Microbiology, Technische Universität München; Munich, Germany: http://www.mikro.biologie.tu-muenchen.de
    [Google Scholar]
  11. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  12. Tindall B. J. 1990; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66:199–202 [CrossRef]
    [Google Scholar]
  13. Ventosa A., Marquez M. C., Kocur M., Tindall B. J. 1993; Comparative study of “ Micrococcus sp.” strains CCM 168 and CCM 1405 and members of the genus Salinicoccus . Int J Syst Bacteriol 43:245–248 [CrossRef]
    [Google Scholar]
  14. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J. 1983; Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813
    [Google Scholar]
  15. Yassin A. F., Sträubler B., Schumann P., Schaal K. P. 2003; Nocardia puris sp. nov. Int J Syst Evol Microbiol 53:1595–1599 [CrossRef]
    [Google Scholar]
  16. Zhang J., Liu Z., Goodfellow M. 2003; Nocardia caishijiensis sp. nov., a novel soil actinomycete. Int J Syst Evol Microbiol 53:999–1004 [CrossRef]
    [Google Scholar]
  17. Ziemke F., Höfle M. G., Lalucat J., Rosselló-Mora R. 1998; Reclassification of Shewanella putrefaciens Owen's genomic group II as Shewanella baltica sp. nov. Int J Syst Bacteriol 48:179–186 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02841-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02841-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error