1887

Abstract

A Gram-negative, oxidase- and catalase-positive, rod-shaped bacterium, designated strain KMM 3655, was isolated from a coastal marine sediment sample. The novel bacterium required sodium ions for growth and grew between 0·5 and 5 % NaCl and at 4–37 °C, but not at 40 °C. It reduced nitrate, formed acids from glucose under aerobic and anaerobic conditions, utilized a limited spectrum of organic substrates and did not produce gelatinase, caseinase, amylase or chitinase. The major isoprenoid quinone was Q8. Polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unknown phospholipid. Fatty acid analysis of strain KMM 3655 revealed C, C 7 and C 7 as predominant components. The G+C content of the DNA was 51·1 mol%. Phylogenetic analysis of the 16S rDNA sequence placed the new isolate within the - as a separate deep branch, with about 90 % sequence similarity to representatives of the genus and other remotely related genera. Combined phylogenetic and physiological data show that the new marine sediment isolate, KMM 3655, represents a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain is KMM 3655 (=DSM 15388).

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2004-05-01
2024-03-29
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References

  1. Baumann P., Baumann L. 1984; Genus Photobacterium Beijerinck 1889. In Bergey's Manual of Systematic Bacteriology vol. 1 pp  539–545 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  2. Baumann L., Baumann P., Mandel M., Allen R. D. 1972; Taxonomy of aerobic marine eubacteria. J Bacteriol 110:402–429
    [Google Scholar]
  3. Baumann P., Furniss A. L., Lee J. V. 1984a; Genus Vibrio Pacini 1854. In Bergey's Manual of Systematic Bacteriology vol 1 pp  518–538 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  4. Baumann P., Gauthier M. J., Baumann L. 1984b; Genus Alteromonas Baumann, Baumann, Mandel and Allen 1972. In Bergey's Manual of Systematic Bacteriology vol 1 pp  343–352 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  5. Bowman J. P., McCammon S. A., Brown J. L., McMeekin T. A. 1998; Glaciecola punicea gen. nov., sp. nov. and Glaciecola pallidula gen. nov., sp. nov.: psychrophilic bacteria from Antarctic sea-ice habitats. Int J Syst Bacteriol 48:1213–1222 [CrossRef]
    [Google Scholar]
  6. Felsenstein J. 1993 phylip (phylogeny inference package), version 3.5c Department of Genetics, University of Washington; Seattle, USA:
    [Google Scholar]
  7. Gauthier G., Gauthier M., Christen R. 1995; Phylogenetic analysis of the genera Alteromonas , Shewanella , and Moritella using genes coding for small-subunit rRNA sequences and division of the genus Alteromonas into two genera, Alteromonas (emended) and Pseudoalteromonas gen. nov., and proposal of twelve new species combinations. Int J Syst Bacteriol 45:755–761 [CrossRef]
    [Google Scholar]
  8. Golyshin P. N., Chernikova T. N., Abraham W.-R., Lünsdorf H., Timmis K. N., Yakimov M. M. 2002; Oleiphilaceae fam nov., to include Oleiphilus messinensis gen. nov., sp. nov., a novel marine bacterium that obligately utilizes hydrocarbons. Int J Syst Evol Microbiol 52:901–911 [CrossRef]
    [Google Scholar]
  9. Gregersen T. 1978; Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5:123–127 [CrossRef]
    [Google Scholar]
  10. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  11. Ivanova E. P., Romanenko L. A., Chun J. 7 other authors 2000; Idiomarina gen. nov., comprising novel indigenous deep-sea bacteria from the Pacific Ocean, including description of two species, Idiomarina abyssalis sp.nov. and Idiomarina zobellii sp. nov. Int J Syst Evol Microbiol 50:901–907 [CrossRef]
    [Google Scholar]
  12. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
    [Google Scholar]
  13. Macián M. C., Ludwig W., Schleifer K. H., Garay E., Pujalte M. J. 2001; Thalassomonas viridans gen. nov., sp. nov., a novel marine γ -proteobacterium. Int J Syst Evol Microbiol 51:1283–1289
    [Google Scholar]
  14. Maidak B. L., Cole J. R., Parker C. T. Jr 11 other authors 1999; A new version of the RDP (Ribosomal Database Project. Nucleic Acids Res 27:171–173 [CrossRef]
    [Google Scholar]
  15. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  16. Mikhailov V. V., Romanenko L. A., Ivanova E. P. 2002; The genus Alteromonas and related Proteobacteria . In The Prokaryotes , 3rd edn, release 3.10. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E. New York: Springer;
    [Google Scholar]
  17. Nguyen B. H., Denner E. B. M., Dang T. C. H., Wanner G., Stan-Lotter H. 1999; Marinobacter aquaeolei sp. nov., a halophilic bacterium isolated from a Vietnamese oil-producing well. Int J Syst Bacteriol 49:367–375 [CrossRef]
    [Google Scholar]
  18. Popoff M. 1984; Genus Aeromonas Kluyver and Van Niel 1936. In Bergey's Manual of Systematic Bacteriology vol. 1 pp  545–548 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  19. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092 [CrossRef]
    [Google Scholar]
  20. Rosselló-Mora R., Ludwig W., Kämpfer P., Amann R., Schleifer K. H. 1995; Ferrimonas balearica gen. nov., sp. nov. a new marine facultative Fe(III)-reducing bacterium. Syst Appl Microbiol 18:196–202 [CrossRef]
    [Google Scholar]
  21. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20:1–6
    [Google Scholar]
  22. Satomi M., Kimura B., Hamada T., Harayama S., Fujii T. 2002; Phylogenetic study of the genus Oceanospirillum based on 16S rRNA and gyrB genes: emended description of the genus Oceanospirillum , description of Pseudospirillum gen.nov., Oceanobacter gen. nov. and Terasakiella gen. nov.and transfer of Oceanospirillum jannaschii and Pseudomonas stanieri to Marinobacterium as Marinobacterium jannaschii comb. nov. and Marinobacterium stanieri comb. nov. Int J Syst Evol Microbiol 52:739–747 [CrossRef]
    [Google Scholar]
  23. Schumann P., Prauser H., Rainey F. A., Stackebrandt E., Hirsch P. 1997; Friedmanniella antarctica gen. nov., sp. nov. an ll-diaminopimelic acid-containing actinomycete from Antarctic sandstone. Int J Syst Bacteriol 47:278–283 [CrossRef]
    [Google Scholar]
  24. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–655 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  25. Van Landschoot A., De Ley J. 1983; Intra- and intergeneric similarities of the rRNA cistrons of Alteromonas Marinomonas (gen. nov.) and some other Gram-negative bacteria. J Gen Microbiol 129:3057–3074
    [Google Scholar]
  26. Venkateswaran K., Dollhopf M. E., Aller R., Stackebrandt E., Nealson K. H. 1998; Shewanella amazonensis sp. nov., a novel metal-reducing facultative anaerobe from Amazonian shelf muds. Int J Syst Bacteriol 48:965–972 [CrossRef]
    [Google Scholar]
  27. Venkateswaran K., Moser D. P., Dollhopf M. E. 10 other authors 1999; Polyphasic taxonomy of the genus Shewanella and description of Shewanella oneidensis sp. nov. Int J Syst Bacteriol 49:705–724 [CrossRef]
    [Google Scholar]
  28. Yakimov M. M., Golyshin P. N., Lang S., Moore E. R. B., Abraham W.-R., Lünsdorf H., Timmis K. N. 1998; Alcanivorax borkumensis gen. nov., sp. nov., a new, hydrocarbon-degrading and surfactant-producing marine bacterium. Int J Syst Bacteriol 48:339–348 [CrossRef]
    [Google Scholar]
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