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Volume 62, Issue 1

Phylogenetic analysis and taxonomic distinction of six genera of pathogenic scuticociliates (Protozoa, Ciliophora) inferred from small-subunit rRNA gene sequences Free

Abstract

Pathogenic scuticociliates, which are common in the haemolymph or tissues of maricultured animals and often cause serious diseases, are a species-rich assemblage with mostly unresolved systematic relationships, especially in some less-studied groups. In the present study, we sequenced the small-subunit rRNA gene of six species of scuticociliates, , , , , and , the last two of which have not been studied previously using molecular analyses. Phylogenetic trees were constructed using Bayesian inference, maximum-likelihood and maximum-parsimony methods to assess the inter- and intra-generic relationships of scuticociliates. Results revealed the following: 1) did not cluster with the Philasteridae, Cohnilembidae or any other family of the order Philasterida; 2) sequences of and showed a difference of 1.02 % (15 nt sites), revealing a close relationship between them; 3) the approximately unbiased test rejected monophyly of both and , indicating that the terminal position of the anterior end of the paroral membrane and the structure of membranelle 1 are unreliable characters for distinction of genera in this group of scuticociliates; 4) grouped with , showing a close phylogenetic relationship between the orders Thigmotrichida and Pleuronematida; and 5) , and should be removed from their currently assigned genera.

  • Published Online:
Funding
This study was supported by the:
  • Natural Science Foundation of China (Award 41006098)
  • King Saud University
© 2012 IUMS
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2024-04-18
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Phylogenetic analysis and taxonomic distinction of six genera of pathogenic scuticociliates (Protozoa, Ciliophora) inferred from small-subunit rRNA gene sequences
Int J Syst Evol Microbiol 62, 246 (2012); https://doi.org/10.1099/ijs.0.028464-0
/content/journal/ijsem/10.1099/ijs.0.028464-0
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