1887

Abstract

A moderately halophilic and alkalitolerant bacterium, designated strain HN30, was isolated from garden soil in Japan. Cells of strain HN30 were motile, endospore-forming, aerobic, rod-shaped and Gram-positive, and contained A1γ -diaminopimelic acid-type murein. Growth occurred in 7–23 % (w/v) NaCl (optimum, 10–15 %, w/v), at pH 6.5–10.0 (optimum, pH 8.0–8.5) and at 20–40 °C (optimum, 30 °C). The isoprenoid quinone was menaquinone-7. The polar lipids were phosphatidylglycerol and diphosphatidylglycerol. The major cellular fatty acids were anteiso-C, anteiso-C, iso-C and C. The DNA G+C content of strain HN30 was 47 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain HN30 was most closely related to BH1 (93 % sequence similarity). 16S rRNA gene sequence similarities with other recognized species were less than 89 %. Phylogenetic and phenotypic characteristics indicated that strain HN30 represents a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed; the type strain is HN30 ( = JCM 15649 = DSM 21771).

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2012-02-01
2024-03-28
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