RT Journal Article SR Electronic(1) A1 Lim, Chae-Sung A1 Oh, Yong-Sik A1 Lee, Jae-Kwan A1 Park, A-Rum A1 Yoo, Jae-Soo A1 Rhee, Sung-Keun A1 Roh, Dong-HyunYR 2011 T1 Flavobacterium chungbukense sp. nov., isolated from soil JF International Journal of Systematic and Evolutionary Microbiology, VO 61 IS 11 SP 2734 OP 2739 DO https://doi.org/10.1099/ijs.0.028563-0 PB Microbiology Society, SN 1466-5034, AB A yellow-pigmented, Gram-staining-negative, non-motile, strictly aerobic and rod-shaped bacterium, designated CS100T, was isolated from soil in Chungbuk, Korea. Phylogenetic analysis and comparative studies based on the 16S rRNA gene sequence showed that strain CS100T belonged to the genus Flavobacterium in the family Flavobacteriaceae. Strain CS100T showed the highest sequence similarities to Flavobacterium glaciei JCM 13953T (97.6 %) and Flavobacterium johnsoniae KACC 11410T (97.1 %). Sequence similarity to other members of the genus Flavobacterium was 91.5–97.0 %. Growth occurred at 4–30 °C, at pH 5.0–9.0 and in the presence of 0–2 % (w/v) NaCl. Flexirubin-type pigments were produced. Menaquinone-6 (MK-6) was the major respiratory quinone and the major fatty acids were iso-C15 : 0 (17.3 %), summed feature 3 (comprising iso-C15 : 0 2-OH and/or C16 : 1ω7c, 15.5 %) and C16 : 0 (11.8 %). The DNA G+C content was 36.4 mol%. Strain CS100T hydrolysed skimmed milk and gelatin, but not chitin or pectin, and showed oxidase and catalase activities. DNA–DNA relatedness was 3.0 % with F. glaciei JCM 13953T and 11.5 % with F. johnsoniae KACC 11410T. On the basis of the evidence from this study, strain CS100T represents a novel species of the genus Flavobacterium, for which the name Flavobacterium chungbukense sp. nov. is proposed. The type strain is CS100T ( = KACC 15048T = JCM 17386T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.028563-0