1887

Abstract

The taxonomic position of an actinomycete isolated from an ultramafic soil in New Caledonia was determined using a polyphasic approach. The isolate, which was designated SFOp68, was shown to have chemical and morphological properties typical of streptomycetes. An almost complete 16S rRNA gene sequence of the isolate was generated and compared with sequences of representative streptomycetes. The 16S rRNA data not only supported the classification of the strain in the genus , but also showed that it formed a distinct phyletic line that was most closely related to one composed of the type strain of . The two organisms can be readily separated using a diverse range of phenotypic properties. It is proposed that strain SFOp68 (=DSM 41836=NCIMB 13954) be classified in the genus as sp. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02891-0
2004-11-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/6/ijs542061.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02891-0&mimeType=html&fmt=ahah

References

  1. Amir H., Pineau R. 1998; Influence of plants and cropping on microbiological characteristics of some New Caledonian ultramafic soils. Aust J Soil Res 361:457–471
    [Google Scholar]
  2. Atalan E., Manfio G. P., Ward A. C., Kroppenstedt R. M., Goodfellow M. 2000; Biosystematic studies on novel streptomycetes from soil. Antonie van Leeuwenhoek 77:337–353 [CrossRef]
    [Google Scholar]
  3. Bérdy J. 1995; Are actinomycetes exhausted as a source of secondary metabolites?. Biotechnologia 7:813–34
    [Google Scholar]
  4. Chun J. 1995; Computer-assisted classification and identification of actinomycetes . PhD thesis University of; Newcastle, UK:
  5. Dieter A., Hamm A., Fiedler H.-P., Goodfellow M., Müller W. E. G., Brun R., Beil W., Bringmann G. 2003; Pyrocoll, an antibiotic, antiparasitic and antitumor compound produced by a novel alkalophilic Streptomyces strain. J Antibiot 56:639–646 [CrossRef]
    [Google Scholar]
  6. Felsenstein J. 1993 phylip (Phylogenetic Inference Package), version 3.5c. Distributed by the author Department of Genetics, University of Washington; Seattle, USA:
    [Google Scholar]
  7. Fitch W. M., Margoliash E. 1967; Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome c sequences is of general applicability. Science 155:279–284 [CrossRef]
    [Google Scholar]
  8. Hayakawa M., Nonomura H. 1987; Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. J Ferment Technol 65:501–509 [CrossRef]
    [Google Scholar]
  9. Jaffré T. 1976; Chemical composition and conditions of mineral supply for plants on ultrabasic rocks. In ORSTOM Notebook, Biology Series II pp  53–63 Paris: ORSTROM (in French;
    [Google Scholar]
  10. Jones K. L. 1949; Fresh isolates of actinomycetes in which the presence of sporogeneous aerial mycelia is a fluctuating characteristic. J Bacteriol 57:141–145
    [Google Scholar]
  11. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol 3 pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  12. Kim S. B., Goodfellow M. 2002; Streptomyces thermospinisporus sp. nov., a moderately thermophilic carboxydotrophic streptomycete isolated from soil. Int J Syst Evol Microbiol 52:1225–1228 [CrossRef]
    [Google Scholar]
  13. Kim S. B., Brown R., Oldfield C., Gilbert S. C., Goodfellow M. 1999; Gordonia desulfuricans sp. nov., a benzothiophene-desulphurizing actinomycete. Int J Syst Bacteriol 49:1845–1851 [CrossRef]
    [Google Scholar]
  14. Kim S. B., Lonsdale J., Seong C.-N., Goodfellow M. 2003; Streptacidiphilus gen. nov., acidophilic actinomycetes with wall chemotype I and emendation of the family Streptomycetaceae (Waksman and Henrici (1943)AL) emend.Rainey et al . 1997. Antonie van Leeuwenhoek 83:107–116 [CrossRef]
    [Google Scholar]
  15. Kluge A. G., Farris F. S. 1969; Quantitative phyletics and the evolution of anurans. Syst Zool 18:1–32 [CrossRef]
    [Google Scholar]
  16. Labeda D. P. 1993; DNA relatedness among strains of the Streptomyces lavendulae phenotypic cluster group. Int J Syst Bacteriol 43:822–825 [CrossRef]
    [Google Scholar]
  17. Labeda D. P. 1998; DNA relatedness among the Streptomyces fulvissimus and Streptomyces griseoviridis phenotypic cluster groups. Int J Syst Bacteriol 48:829–832 [CrossRef]
    [Google Scholar]
  18. Labeda D. P., Lyons A. J. 1992; DNA relatedness among strains of the sweet potato pathogen Streptomyces ipomoea (Person and Martin 1940) Waksman and Henrici 1948. Appl Environ Microbiol 58:532–535
    [Google Scholar]
  19. Lechevalier M. P., Lechevalier H. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Evol Microbiol 20:435–443
    [Google Scholar]
  20. Li W., Lanoot B., Zhang Y., Vancanneyt M., Swings J., Liu Z. 2002; Streptomyces scopiformis sp. nov., a novel streptomycete with fastigiate spore chains. Int J Syst Evol Microbiol 52:1629–1633 [CrossRef]
    [Google Scholar]
  21. Manfio G. P., Zakrzewska-Czerwinska J., Atalan E., Goodfellow M. 1995; Towards minimal standards for the description of Streptomyces species. Biotechnologia 7:8242–253
    [Google Scholar]
  22. Manfio G. P., Atalan E., Zakrzewska-Czerwinska J., Mordarski M., Rodriguez C., Collins M. D., Goodfellow M. 2003; Classification of novel soil streptomycetes as Streptomyces aureus sp.nov., Streptomyces laceyi sp. nov. and Streptomyces sanglieri sp. nov. Antonie van Leeuwenhoek 83:245–255 [CrossRef]
    [Google Scholar]
  23. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  24. O'Donnell A. G., Falconer C., Goodfellow M., Ward A. C., Williams E. 1993; Biosystematics and diversity amongst novel carboxydotrophic actinomycetes. Antonie van Leeuwenhoek 64:325–340
    [Google Scholar]
  25. Saintpierre D. 2001; Identification of novel actinomycete strains from New-Caledonian ultramafic soils. Chemical characterization of some antibiotic products . PhD thesis Institut National Polytechnique de Toulouse; France: (in French
  26. Saintpierre D., Amir H., Pineau R., Sembiring L., Goodfellow M. 2003; Streptomyces yatensis sp. nov., a novel bioactive streptomycete isolated from a New-Caledonian ultramafic soil. Antonie van Leeuwenhoek 83:21–26 [CrossRef]
    [Google Scholar]
  27. Saintpierre-Bonaccio D., Maldonado L. A., Amir H., Pineau R., Goodfellow M. 2004; Nocardia neocaledoniensis sp. nov., a novel actinomycete isolated from a New-Caledonian brown hypermagnesian soil. Int J Syst Evol Microbiol 54:599–603 [CrossRef]
    [Google Scholar]
  28. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  29. Sembiring L., Ward A. C., Goodfellow M. 2000; Selective isolation and characterisation of members of the Streptomyces violaceusniger clade associated with roots of Paraserianthes falcataria . Antonie van Leeuwenhoek 78:353–366 [CrossRef]
    [Google Scholar]
  30. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  31. Shirling E. B., Gottlieb D. 1968; Cooperative description of the type strains of Streptomyces . III. Additional species descriptions from the first and second studies. Int J Syst Bacteriol 18:279–392 [CrossRef]
    [Google Scholar]
  32. Sierra G. 1957; A simple method for detection of lipolytic activity of microorganisms and some observations on the influence of the contact between cells and fatty substrates. Antonie van Leeuwenhoek 23:15–22 [CrossRef]
    [Google Scholar]
  33. Staneck J. L., Roberts G. D. 1974; Simplified approach to the identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  34. Waksman S. A., Henrici A. T. 1943; The nomenclature and classification of the actinomycetes. J Bacteriol 46:337–341
    [Google Scholar]
  35. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J. 1983; Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1742–1813
    [Google Scholar]
  36. Williams S. T., Goodfellow M., Alderson G. 1989; Genus Streptomyces Waksman and Henrici 1943, 339AL . In Bergey's Manual of Systematic Bacteriology vol. 4 pp  2452–2492 Edited by Williams S. T., Sharpe M. E, Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02891-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02891-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error