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Volume 62, Issue Pt_6

Phylogenetic analysis of based on partial gene sequences and species differentiation by PCR-RFLP Free

Abstract

The gene was evaluated as an alternative molecular marker for the differentiation of species and in order to understand better the phylogenetic relationships within the genus. PCR-RFLP experiments using III allowed differentiation of species, particularly those that affect the same plant host such as and , pathogenic to sugar cane, and , which cause disease in melon, and , and /, pathogenic to tomato. Phylogenetic relationships within the genus were also examined by comparing partial gene sequences (612 nt) and the species were separated into two main groups. Group I, well supported by bootstrap values of 99 %, comprised , , , , , , , , , , , , , , , , , , , and Group II, again well supported by bootstrap values of 99 %, comprised , , , and . The gene sequence similarity observed among the species in this study ranged from 87.8 to 99.7 %. The results of PCR-RFLP of the gene indicated that this technique can be used for diagnosis and identification of most strains, including closely related species within the genus. However, species that showed identical profiles could be differentiated clearly only by sequence analysis. The results obtained in our phylogenetic analysis suggested that the gene can be used as an alternative molecular marker for genetic relatedness in the genus . The results of PCR-RFLP of the gene indicate that this technique can be used for diagnosis and identification of closely related species within the genus, representing a rapid and inexpensive tool that can be easily standardized between laboratories.

  • Published Online:
Funding
This study was supported by the:
  • Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
© 2012 IUMS
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2012-06-01
2024-04-25
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Phylogenetic analysis of Xanthomonas based on partial rpoB gene sequences and species differentiation by PCR-RFLP
Int J Syst Evol Microbiol 62, 1419 (2012); https://doi.org/10.1099/ijs.0.028977-0
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