@article{mbs:/content/journal/ijsem/10.1099/ijs.0.02903-0, author = "Zhang, Chunyang and Liu, Xiaoli and Dong, Xiuzhu", title = "Syntrophomonas curvata sp. nov., an anaerobe that degrades fatty acids in co-culture with methanogens", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2004", volume = "54", number = "3", pages = "969-973", doi = "https://doi.org/10.1099/ijs.0.02903-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.02903-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "UASB, up-flow anaerobic sludge blanket", keywords = "FAME, fatty acid methyl ester", abstract = "A strict anaerobe (strain GB8-1T) that degraded straight-chain fatty acids with C4–C18 in syntrophic association with methanogens was isolated from an up-flow anaerobic sludge blanket reactor treating beer wastewater. Strain GB8-1T degraded 1 mol butyrate into about 2 mol acetate and 1 mol (presumably) H2 in co-culture with a methanogen. Neither branched-chain fatty acids nor benzoate could be degraded. Strain GB8-1T could grow on crotonate in pure culture and converted 1 mol crotonate to 0·5 mol butyrate and 1 mol acetate. Generation time was about 11 h when grown on crotonate at 37 °C. Fumarate, sulfate, thiosulfate, sulfur and nitrate could not serve as electron acceptors for strain GB8-1T to degrade butyrate. Cells of strain GB8-1T were curved rods with Gram-negative cell walls; no spores were observed. The DNA G+C content was 46·6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain GB8-1T was related most closely to the fatty acid-oxidizing, syntrophic bacterium Syntrophomonas sapovorans DSM 3441T; however, the relationship was not very close (95·4 % sequence similarity). Some phenotypic features also differentiated strain GB8-1T from Syntrophomonas sapovorans DSM 3441T. Therefore, a novel species, Syntrophomonas curvata sp. nov., is proposed. The type strain is GB8-1T (=CGMCC 1.5010T=DSM 15682T).", }