1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 62, Issue 2

sp. nov., isolated from feline gastric mucosa Free

Abstract

Three Gram-negative, microaerophilic bacteria, strains ASB1, ASB2 and ASB3, with a corkscrew-like morphology isolated from the gastric mucosa of cats were studied using a polyphasic taxonomic approach. The isolates grew on biphasic culture plates under microaerobic conditions at 37 °C and exhibited urease, oxidase and catalase activities. They were also able to grow in colonies on dry agar plates. Based on 16S rRNA gene sequence analysis, ASB1, ASB2 and ASB3 were identified as members of the genus and showed 98 to 99 % sequence similarity to strains of , , ‘ Helicobacter heilmannii’, , and , six related species previously detected in feline or canine gastric mucosa. Sequencing of the partial gene demonstrated that ASB1, ASB2 and ASB3 constitute a separate taxon among the feline and canine species. The urease gene sequences of ASB1, ASB2 and ASB3 showed approximately 91 % similarity to those of ‘ Helicobacter heilmannii’. Protein profiling, the absence of alkaline phosphatase activity and several other biochemical characteristics also allowed strains ASB1, ASB2 and ASB3 to be differentiated from other species of feline or canine gastric origin. The results of this polyphasic taxonomic study show that the cultured isolates constitute a new taxon corresponding to ‘ Helicobacter heilmannii’, which was previously demonstrated in the stomach of humans, wild felidae, cats and dogs. The name sp. nov. is proposed for these isolates; the type strain is ASB1 ( = DSM 23983 = LMG 26292).

  • Published Online:
Funding
This study was supported by the:
  • Research Fund of Ghent University, Belgium (Award GOA01G00408)
© 2012 IUMS

Erratum

An erratum has been published for this content:
sp. nov., isolated from feline gastric mucosa
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.029207-0
2012-02-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/2/299.html?itemId=/content/journal/ijsem/10.1099/ijs.0.029207-0&mimeType=html&fmt=ahah

References

  1. Baele M., Devriese L. A., Haesebrouck F. 2001; Lactobacillus agilis is an important component of the pigeon crop flora. J Appl Microbiol 91:488–491 [View Article][PubMed]
     [Google Scholar]
  2. Baele M., Decostere A., Vandamme P., Ceelen L., Hellemans A., Mast J., Chiers K., Ducatelle R., Haesebrouck F. 2008a; Isolation and characterization of Helicobacter suis sp. nov. from pig stomachs. Int J Syst Evol Microbiol 58:1350–1358 [View Article][PubMed]
     [Google Scholar]
  3. Baele M., Decostere A., Vandamme P., Van den Bulck K., Gruntar I., Mehle J., Mast J., Ducatelle R., Haesebrouck F. 2008b; Helicobacter baculiformis sp. nov., isolated from feline stomach mucosa. Int J Syst Evol Microbiol 58:357–364 [View Article][PubMed]
     [Google Scholar]
  4. Coenye T., Falsen E., Vancanneyt M., Hoste B., Govan J. R. W., Kersters K., Vandamme P. 1999; Classification of Alcaligenes faecalis-like isolates from the environment and human clinical samples as Ralstonia gilardii sp. nov.. Int J Syst Bacteriol 49:405–413 [View Article][PubMed]
     [Google Scholar]
  5. Dewhirst F. E., Fox J. G., On S. L. W. 2000; Recommended minimal standards for describing new species of the genus Helicobacter . Int J Syst Evol Microbiol 50:2231–2237 [View Article][PubMed]
     [Google Scholar]
  6. Gruntar I., Mehle J., Krt B., Ocepek M., Gombac M., Cerne M., Pogacnik M. 2003; New approaches in isolation of Helicobacter spp. from gastric mucosa in dogs. Int J Med Microbiol 293:Suppl.65
     [Google Scholar]
  7. Haesebrouck F., Pasmans F., Flahou B., Chiers K., Baele M., Meyns T., Decostere A., Ducatelle R. 2009; Gastric helicobacters in domestic animals and nonhuman primates and their significance for human health. Clin Microbiol Rev 22:202–223 [View Article][PubMed]
     [Google Scholar]
  8. Hänninen M. L., Happonen I., Saari S., Jalava K. 1996; Culture and characteristics of Helicobacter bizzozeronii, a new canine gastric Helicobacter sp.. Int J Syst Bacteriol 46:160–166 [View Article][PubMed]
     [Google Scholar]
  9. Happonen I., Saari S., Castren L., Tyni O., Hänninen M. L., Westermarck E. 1996; Occurrence and topographical mapping of gastric Helicobacter-like organisms and their association with histological changes in apparently healthy dogs and cats. Zentralbl Veterinarmed A 43:305–315[PubMed] [CrossRef]
     [Google Scholar]
  10. Heilmann K. L., Borchard F. 1991; Gastritis due to spiral shaped bacteria other than Helicobacter pylori: clinical, histological, and ultrastructural findings. Gut 32:137–140 [View Article][PubMed]
     [Google Scholar]
  11. Houf K., On S. L. W., Coenye T., Mast J., Van Hoof J., Vandamme P. 2005; Arcobacter cibarius sp. nov., isolated from broiler carcasses. Int J Syst Evol Microbiol 55:713–717 [View Article][PubMed]
     [Google Scholar]
  12. Hwang C. Y., Han H. R., Youn H. Y. 2002; Prevalence and clinical characterization of gastric Helicobacter species infection of dogs and cats in Korea. J Vet Sci 3:123–133[PubMed]
     [Google Scholar]
  13. Jalava K., Kaartinen M., Utriainen M., Happonen I., Hänninen M. L. 1997; Helicobacter salomonis sp. nov., a canine gastric Helicobacter sp. related to Helicobacter felis and Helicobacter bizzozeronii . Int J Syst Bacteriol 47:975–982 [View Article][PubMed]
     [Google Scholar]
  14. Jalava K., On S. L. W., Vandamme P. A. R., Happonen I., Sukura A., Hänninen M. L. 1998; Isolation and identification of Helicobacter spp. from canine and feline gastric mucosa. Appl Environ Microbiol 64:3998–4006[PubMed]
     [Google Scholar]
  15. Jalava K., On S. L. W., Harrington C. S., Andersen L. P., Hänninen M. L., Vandamme P. 2001; A cultured strain of ‘Helicobacter heilmannii’, a human gastric pathogen, identified as H. bizzozeronii: evidence for zoonotic potential of Helicobacter . Emerg Infect Dis 7:1036–1038 [View Article][PubMed]
     [Google Scholar]
  16. Lee A., Hazell S. L., O’Rourke J., Kouprach S. 1988; Isolation of a spiral-shaped bacterium from the cat stomach. Infect Immun 56:2843–2850[PubMed]
     [Google Scholar]
  17. Mast J., Nanbru C., van den Berg T., Meulemans G. 2005; Ultrastructural changes of the tracheal epithelium after vaccination of day-old chickens with the La Sota strain of Newcastle disease virus. Vet Pathol 42:559–565 [View Article][PubMed]
     [Google Scholar]
  18. Mikkonen T. P., Kärenlampi R. I., Hänninen M. L. 2004; Phylogenetic analysis of gastric and enterohepatic Helicobacter species based on partial HSP60 gene sequences. Int J Syst Evol Microbiol 54:753–758 [View Article][PubMed]
     [Google Scholar]
  19. Neiger R., Dieterich C., Burnens A., Waldvogel A., Corthésy-Theulaz I., Halter F., Lauterburg B., Schmassmann A. 1998; Detection and prevalence of Helicobacter infection in pet cats. J Clin Microbiol 36:634–637[PubMed]
     [Google Scholar]
  20. O’Rourke J. L., Dixon M. F., Jack A., Enno A., Lee A. 2004a; Gastric B-cell mucosa-associated lymphoid tissue (MALT) lymphoma in an animal model of ‘Helicobacter heilmannii’ infection. J Pathol 203:896–903 [View Article][PubMed]
     [Google Scholar]
  21. O’Rourke J. L., Solnick J. V., Neilan B. A., Seidel K., Hayter R., Hansen L. M., Lee A. 2004b; Description of ‘Candidatus Helicobacter heilmannii’ based on DNA sequence analysis of 16S rRNA and urease genes. Int J Syst Evol Microbiol 54:2203–2211 [View Article][PubMed]
     [Google Scholar]
  22. Pot B., Devriese L. A., Hommez J., Miry C., Vandemeulebroecke K., Kersters K., Haesebrouck F. 1994; Characterization and identification of Vagococcus fluvialis strains isolated from domestic animals. J Appl Bacteriol 77:362–369 [View Article][PubMed]
     [Google Scholar]
  23. Solnick J. V., O’Rourke J., Lee A., Paster B. J., Dewhirst F. E., Tompkins L. S. 1993; An uncultured gastric spiral organism is a newly identified Helicobacter in humans. J Infect Dis 168:379–385 [View Article][PubMed]
     [Google Scholar]
  24. Trebesius K., Adler K., Vieth M., Stolte M., Haas R. 2001; Specific detection and prevalence of Helicobacter heilmannii-like organisms in the human gastric mucosa by fluorescent in situ hybridization and partial 16S ribosomal DNA sequencing. J Clin Microbiol 39:1510–1516 [View Article][PubMed]
     [Google Scholar]
  25. Van den Bulck K., Decostere A., Baele M., Driessen A., Debongnie J. C., Burette A., Stolte M., Ducatelle R., Haesebrouck F. 2005; Identification of non-Helicobacter pylori spiral organisms in gastric samples from humans, dogs, and cats. J Clin Microbiol 43:2256–2260 [View Article][PubMed]
     [Google Scholar]
  26. Van den Bulck K., Decostere A., Baele M., Vandamme P., Mast J., Ducatelle R., Haesebrouck F. 2006; Helicobacter cynogastricus sp. nov., isolated from the canine gastric mucosa. Int J Syst Evol Microbiol 56:1559–1564 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.029207-0
Loading
Helicobacter heilmannii sp. nov., isolated from feline gastric mucosa
Int J Syst Evol Microbiol 62, 299 (2012); https://doi.org/10.1099/ijs.0.029207-0
/content/journal/ijsem/10.1099/ijs.0.029207-0
/content/journal/ijsem/10.1099/ijs.0.029207-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error