1887

Abstract

A Gram-stain-negative, non-motile, non-spore-forming bacterial strain, YCS-5, was isolated from seawater off the southern coast of Korea. Strain YCS-5 grew optimally at 30 °C and in the presence of 2 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain YCS-5 fell within the clade comprising species. Strain YCS-5 exhibited 16S rRNA gene sequence similarity values of 96.6, 95.7 and 97.9 % to the type strains of , and , respectively, and less than 89.8 % to strains of other species used in the phylogenetic analysis. Strain YCS-5 contained Q-8 as the predominant ubiquinone and iso-C, iso-C, iso-C 3-OH and iso-Cω9 as the major fatty acids. The polar lipid profile of strain YCS-5 was similar to that of . SW-125, with phosphatidylglycerol and an unidentified aminolipid as major polar lipids. The DNA G+C content was 47 mol%. The mean DNA–DNA relatedness value between strain YCS-5 and . JCM 16211 was 12 %. Differential phenotypic properties and the phylogenetic and genetic distinctiveness of strain YCS-5 demonstrated that this strain is distinguishable from other species. On the basis of the data presented, strain YCS-5 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed; the type strain is YCS-5 ( = KCTC 23420 = CCUG 60526).

Funding
This study was supported by the:
  • Program for Collection, Management and Utilization of Biological Resources (Award M10867010003)
  • 21C Frontier Program of Microbial Genomics and Applications (Award 11-2008-00-002-00)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.029314-0
2012-03-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/3/511.html?itemId=/content/journal/ijsem/10.1099/ijs.0.029314-0&mimeType=html&fmt=ahah

References

  1. Ahn J., Park J.-W., McConnell J. A., Ahn Y.-B., Häggblom M. M. 2011; Kangiella spongicola sp. nov., a halophilic marine bacterium isolated from the marine sponge Chondrilla nucula. . Int J Syst Evol Microbiol 61:961–964 [CrossRef]
    [Google Scholar]
  2. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006 [View Article][PubMed]
    [Google Scholar]
  3. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
    [Google Scholar]
  5. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
    [Google Scholar]
  6. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67 [View Article]
    [Google Scholar]
  7. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184[PubMed]
    [Google Scholar]
  8. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
    [Google Scholar]
  9. Romanenko L. A., Tanaka N., Frolova G. M., Mikhailov V. V. 2010; Kangiella japonica sp. nov., isolated from a marine environment. Int J Syst Evol Microbiol 60:2583–2586 [View Article][PubMed]
    [Google Scholar]
  10. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc;
  11. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
    [Google Scholar]
  12. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
    [Google Scholar]
  13. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
  14. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [View Article]
    [Google Scholar]
  15. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [View Article][PubMed]
    [Google Scholar]
  16. Yoon J.-H., Oh T.-K., Park Y.-H. 2004; Kangiella koreensis gen. nov., sp. nov. and Kangiella aquimarina sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 54:1829–1835 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.029314-0
Loading
/content/journal/ijsem/10.1099/ijs.0.029314-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error