1887

Abstract

It was shown recently that the genus is rich in N-fixing bacteria that are associated with plants. A group of these diazotrophic isolates with identical or very similar 16S rDNA restriction patterns [designated amplified rDNA restriction analysis (ARDRA) genotypes 13, 14 and 15] was selected and a polyphasic taxonomic study was performed, which included new isolates that were recovered from rhizospheres, rhizoplanes or internal tissues of maize, sugarcane and coffee plants. Morphological, physiological and biochemical features, as well as multi-locus enzyme electrophoresis profiles and whole-cell protein patterns, of 20 strains were analysed. In addition, analysis of cellular fatty acid profiles, 16S rDNA sequence analysis and DNA–DNA reassociation experiments were performed with representative strains. The taxonomic data indicated that the strains analysed belong to a novel diazotrophic species, for which the name sp. nov. is proposed. Strain MTl-641 (=ATCC BAA-744=CIP 107921), isolated from the rhizosphere of maize, was designated as the type strain. was found as an endophyte of plants grown in regions with climates ranging from semi-hot subhumid to hot humid, but not from plants grown in regions with semi-hot or hot dry climates. Moreover, was isolated from rhizospheres and plants growing in soils with pH values in the range 4·5–7·1, but not from soils with pH values higher than 7·5.

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2004-07-01
2024-03-28
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References

  1. Achouak W., Christen R., Barakat M., Martel M.-H., Heulin T. 1999; Burkholderia caribensis sp. nov., an exopolysaccharide-producing bacterium isolated from vertisol microaggregates in Martinique. Int J Syst Bacteriol 49:787–794 [CrossRef]
    [Google Scholar]
  2. Brämer C. O., Vandamme P., da Silva L. F., Gomez J. G. C., Steinbüchel A. 2001; Burkholderia sacchari sp. nov., a polyhydroxyalkanoate-accumulating bacterium isolated from soil of a sugar-cane plantation in Brazil. Int J Syst Evol Microbiol 51:1709–1713 [CrossRef]
    [Google Scholar]
  3. Brett P. J., DeShazer D., Woods D. E. 1998; Burkholderia thailandensis sp. nov., a Burkholderia pseudomallei -like species. Int J Syst Bacteriol 48:317–320 [CrossRef]
    [Google Scholar]
  4. Caballero-Mellado J., Fuentes-Ramirez L. E., Reis V. M., Martinez-Romero E. 1995; Genetic structure of Acetobacter diazotrophicus populations and identification of a new genetically distant group. Appl Environ Microbiol 61:3008–3013
    [Google Scholar]
  5. Coenye T., Vandamme P. 2003; Diversity and significance of Burkholderia species occupying diverse ecological niches. Environ Microbiol 5:719–729 [CrossRef]
    [Google Scholar]
  6. Coenye T., Laevens S., Willems A., Ohlén M., Hannant W., Govan J. R. W., Gillis M., Falsen E., Vandamme P. 2001a; Burkholderia fungorum sp. nov. and Burkholderia caledonica sp. nov., two new species isolated from the environment, animals and human clinical samples. Int J Syst Evol Microbiol 51:1099–1107 [CrossRef]
    [Google Scholar]
  7. Coenye T., Mahenthiralingam E., Henry D., LiPuma J. J., Laevens S., Gillis M., Speert D. P., Vandamme P. 2001b; Burkholderia ambifaria sp. nov., a novel member of the Burkholderia cepacia complex including biocontrol and cystic fibrosis-related isolates. Int J Syst Evol Microbiol 51:1481–1490
    [Google Scholar]
  8. Estrada-de los Santos P., Bustillos-Cristales R., Caballero-Mellado J. 2001; Burkholderia , a genus rich in plant-associated nitrogen fixers with wide environmental and geographic distribution. Appl Environ Microbiol 67:2790–2798 [CrossRef]
    [Google Scholar]
  9. Gillis M., Van T. V., Bardin R. 7 other authors 1995; Polyphasic taxonomy in the genus Burkholderia leading to an emended description of the genus and proposition of Burkholderia vietnamiensis sp. nov. for N2-fixing isolates from rice in Vietnam. Int J Syst Bacteriol 45:274–289 [CrossRef]
    [Google Scholar]
  10. Goris J., Dejonghe W., Falsen E., De Clerck E., Geeraerts B., Willems A., Top E. M., Vandamme P., De Vos P. 2002; Diversity of transconjugants that acquired plasmid pJP4 or pEMT1 after inoculation of a donor strain in the A- and B-horizon of an agricultural soil and description of Burkholderia hospita sp.nov. and Burkholderia terricola sp. nov. Syst Appl Microbiol 25:340–352 [CrossRef]
    [Google Scholar]
  11. Henry D. A., Campbell M. E., LiPuma J. J., Speert D. P. 1997; Identification of Burkholderia cepacia isolates from patients with cystic fibrosis and use of a simple new selective medium. J Clin Microbiol 35:614–619
    [Google Scholar]
  12. Kumar S., Tamura K., Jakobsen I. B., Nei M. 2001; mega2: molecular evolutionary genetic analysis software. Bioinformatics 17:1244–1245
    [Google Scholar]
  13. Martínez-Romero E., Caballero-Mellado J. 1996; Rhizobium phylogenies and bacterial genetic diversity. Crit Rev Plant Sci 15:113–140 [CrossRef]
    [Google Scholar]
  14. Reis V. M., Estrada-de los Santos P., Tenorio-Salgado S. 10 other authors 2004; Burkholderia tropica sp. nov., a novel nitrogen-fixing, plant-associated bacterium. Int J Syst Evol Microbiol (in press
  15. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  16. Schwyn B., Neilands J. B. 1987; Universal chemical assay for the detection and determination of siderophores. Anal Biochem 160:47–56 [CrossRef]
    [Google Scholar]
  17. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  18. Vandamme P., Pot B., Gillis M., de Vos P., Kersters K., Swings J. 1996; Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiol Rev 60:407–438
    [Google Scholar]
  19. Vandamme P., Holmes B., Vancanneyt M. 8 other authors 1997; Occurrence of multiple genomovars of Burkholderia cepacia in cystic fibrosis patients and proposal of Burkholderia multivorans sp. nov. Int J Syst Bacteriol 47:1188–1200 [CrossRef]
    [Google Scholar]
  20. Vandamme P., Goris J., Chen W.-M., de Vos P., Willems A. 2002a; Burkholderia tuberum sp. nov. and Burkholderia phymatum sp. nov., nodulate the roots of tropical legumes. Syst Appl Microbiol 25:507–512 [CrossRef]
    [Google Scholar]
  21. Vandamme P., Henry D., Coenye T., Nzula S., Vancanneyt M., LiPuma J. J., Speert D. P., Govan J. R. W., Mahenthiralingam E. 2002b; Burkholderia anthina sp. nov. and Burkholderia pyrrocinia , two additional Burkholderia cepacia complex bacteria, may confound results of new molecular diagnostic tools. FEMS Immunol Med Microbiol 33:143–149 [CrossRef]
    [Google Scholar]
  22. Viallard V., Poirier I., Cournoyer B., Haurat J., Wiebkin S., Ophel-Keller K., Balandreau J. 1998; Burkholderia graminis sp. nov., a rhizospheric Burkholderia species, and reassessment of[ Pseudomonas ] phenazinium , [ Pseudomonas ] pyrrocinia and [ Pseudomonas ] glathei as Burkholderia . Int J Syst Bacteriol 48:549–563 [CrossRef]
    [Google Scholar]
  23. Yabuuchi E., Kosako Y., Oyaizu H., Yano I., Hotta H., Hashimoto Y., Ezaki T., Arakawa M. 1992; Proposal of Burkholderia gen. nov. and transfer of seven species of the genus Pseudomonas homology group II to the new genus, with the type species Burkholderia cepacia (Palleroni and Holmes 1981) comb. nov. Microbiol Immunol 36:1251–1275 [CrossRef]
    [Google Scholar]
  24. Yabuuchi E., Kawamura Y., Ezaki T., Ikedo M., Dejsirilert S., Fujiwara N., Naka T., Kobayashi K. 2000a; Burkholderia uboniae sp. nov., l-arabinose-assimilating but different from Burkholderia thailandensis and Burkholderia vietnamiensis . Microbiol Immunol 44:307–317 [CrossRef]
    [Google Scholar]
  25. Yabuuchi E., Kawamura Y., Ezaki T., Ikedo M., Dejsirilert S., Fujiwara N., Naka T., Kobayashi K. 2000b; Burkholderia ubonensis corrig. ( Burkholderia uboniae [sic]). In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB , List No. 75. Int J Syst Bacteriol 50:1415–1417 [CrossRef]
    [Google Scholar]
  26. Zhang H., Hanada S., Shigematsu T., Shibuya K., Kamagata Y., Kanagawa T., Kurane R. 2000; Burkholderia kururiensis sp. nov., a trichloroethylene (TCE)-degrading bacterium isolated from an aquifer polluted with TCE. Int J Syst Evol Microbiol 50:743–749 [CrossRef]
    [Google Scholar]
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