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Volume 54, Issue 6

gen. nov., sp. nov., a novel member of the –– branch Free

Abstract

Gram-negative, non-spore-forming, rod-shaped, yellow-pigmented bacteria isolated from a freshwater stream in Korea were investigated to determine their taxonomic position. Complete 16S rRNA gene sequence analysis indicated that the organisms should be placed in the –– branch in the family . Phylogenetically, the strains were most closely related to ATCC 33487 and LMG 13028 (94·3 and 94·1 % 16S rRNA gene sequence similarity, respectively) and they clustered on a separate well-supported branch. The strains contained menaquinone MK-6 as the predominant respiratory quinone and showed higher G+C contents (41·7 mol%) than other species in the –– branch and i-C as a major fatty acid (47–52 %). The phylogenetic distances from any species with validly published names and their phenotypic properties confirmed that the strains constitute a separate species in a new genus, for which the name gen. nov., sp. nov. is proposed (type strain Chj707=KCTC 12107=IAM 15050).

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Keyword(s): CFB, Cytophaga–Flavobacterium–Bacteroides and FAME, fatty acid methyl ester
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2004-11-01
2024-04-18
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References

  1. Bernardet J.-F., Segers P., Vancanneyt M., Berthe F., Kersters K., Vandamme P. 1996; Cutting a Gordian knot: emended classification and description of the genus Flavobacterium , emended description of the family Flavobacteriaceae , and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46:128–148 [CrossRef]
     [Google Scholar]
  2. Buck J. D. 1982; Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. Appl Environ Microbiol 44:992–993
     [Google Scholar]
  3. Busse H.-J., Denner E. B. M., Lubitz W. 1996; Classification and identification of bacteria: current approaches to an old problem. Overview of methods used in bacterial systematics. J Biotechnol 47:3–38 [CrossRef]
     [Google Scholar]
  4. Collins M. D., Jones D. 1981; Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implications. Microbiol Rev 45:316–354
     [Google Scholar]
  5. Dees S. B., Carlone G. M., Hollis D., Moss C. W. 1985; Chemical and phenotypic characteristics of Flavobacterium thalpophilum compared with those of other Flavobacterium and Sphingobacterium species. Int J Syst Bacteriol 35:16–22 [CrossRef]
     [Google Scholar]
  6. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
     [Google Scholar]
  7. Holmes B. 1992; The genera Flavobacterium , Sphingobacterium , and Weeksella . In The Prokaryotes , 2nd edn. vol 4 pp  3620–3630 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. Berlin: Springer;
     [Google Scholar]
  8. Holmes B., Owen R. J., Steigerwalt A. G., Brenner D. J. 1984; Flavobacterium gleum , a new species found in human clinical specimens. Int J Syst Bacteriol 34:21–25 [CrossRef]
     [Google Scholar]
  9. Holmes B., Steigerwalt A. G., Weaver R. E., Brenner D. J. 1986; Weeksella zoohelcum sp. nov. (formerly group IIj) from human clinical specimens. Syst Appl Microbiol 8:191–196 [CrossRef]
     [Google Scholar]
  10. Hugh R., Leifson E. 1953; The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram-negative bacteria. J Bacteriol 66:24–26
     [Google Scholar]
  11. Hugo C. J., Jooste P. J., Segers P., Vancanneyt M., Kersters K. 1999; A polyphasic taxonomic study of Chryseobacterium strains isolated from dairy sources. Syst Appl Microbiol 22:586–595 [CrossRef]
     [Google Scholar]
  12. Hugo C. J., Segers P., Hoste B., Vancanneyt M., Kersters K. 2003; Chryseobacterium joostei sp. nov., isolated from the dairy environment. Int J Syst Evol Microbiol 53:771–777 [CrossRef]
     [Google Scholar]
  13. Jooste P. J., Hugo C. J. 1999; The taxonomy, ecology and cultivation of bacterial genera belonging to the family Flavobacteriaceae . Int J Food Microbiol 53:81–94 [CrossRef]
     [Google Scholar]
  14. Kämpfer P., Dreyer U., Neef A., Dott W., Busse H.-J. 2003; Chryseobacterium defluvii sp. nov., isolated from wastewater. Int J Syst Evol Microbiol 53:93–97 [CrossRef]
     [Google Scholar]
  15. Kumar S., Tamura K., Jacobsen I. B., Nei M. 2001; mega2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245 [CrossRef]
     [Google Scholar]
  16. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
     [Google Scholar]
  17. Mudarris M., Austin B., Segers P., Vancanneyt M., Hoste B., Bernardet J. F. 1994; Flavobacterium scophthalmum sp. nov., a pathogen of turbot ( Scophthalmus maximus L.). Int J Syst Bacteriol 44:447–453 [CrossRef]
     [Google Scholar]
  18. Rosselló-Mora R., Amann R. 2001; The species concept for prokaryotes. FEMS Microbiol Rev 25:39–67 [CrossRef]
     [Google Scholar]
  19. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
     [Google Scholar]
  20. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids . MIDI Technical Note 101: Newark, DE: MIDI Inc;
     [Google Scholar]
  21. Schleifer K. H., Stackebrandt E. 1983; Molecular systematics of prokaryotes. Annu Rev Microbiol 37:143–187 [CrossRef]
     [Google Scholar]
  22. Segers P., Mannheim W., Vancanneyt M., De Brandt K., Hinz K.-H., Kersters K., Vandamme P. 1993; Riemerella anatipestifer gen. nov., comb. nov., the causative agent of septicemia anserum exsudativa, and its phylogenetic affiliation within the Flavobacterium - Cytophaga rRNA homology group. Int J Syst Bacteriol 43:768–776 [CrossRef]
     [Google Scholar]
  23. Shin Y. K., Lee J.-S., Chun C. O., Kim H.-J., Park Y.-H. 1996; Isoprenoid quinone profiles of the Leclercia adecarboxylata KCTC 1036T . J Microbiol Biotechnol 6:68–69
     [Google Scholar]
  24. Stackebrandt E., Liesack W. 1993; Nucleic acids and classification. In Handbook of New Bacterial Systematics pp  151–194 Edited by Goodfellow M., O'Donnell A. G. London: Academic Press;
     [Google Scholar]
  25. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
     [Google Scholar]
  26. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
     [Google Scholar]
  27. Vancanneyt M., Vandamme P., Segers P., Torck U., Coopman R., Kersters K., Hinz K.-H. 1999; Riemerella columbina sp. nov., a bacterium associated with respiratory disease in pigeons. Int J Syst Bacteriol 49:289–295 [CrossRef]
     [Google Scholar]
  28. Vandamme P., Bernardet J.-F., Segers P., Kersters K., Holmes B. 1994; New perspectives in the classification of the flavobacteria: description of Chryseobacterium gen. nov., Bergeyella gen. nov., and Empedobacter nom. rev. Int J Syst Bacteriol 44827–831 [CrossRef]
     [Google Scholar]
  29. Vandamme P., Pot B., Gillis M., De Vos P., Kersters K., Swings J. 1996; Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiol Rev 60:407–438
     [Google Scholar]
  30. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703
     [Google Scholar]
  31. Woese C. R., Yang D., Mandelco L., Stetter K. O. 1990; The Flexibacter - Flavobacterium connection. Syst Appl Microbiol 13:161–165 [CrossRef]
     [Google Scholar]
  32. Yabuuchi E., Kaneko T., Yano I., Moss C. W., Miyoshi N. 1983; Sphingobacterium gen. nov., Sphingobacterium spiritivorum comb.nov., Sphingobacterium multivorum comb. nov., Sphingobacterium mizutae sp. nov., and Flavobacterium indologenes sp. nov. glucose-nonfermenting gram-negative rods in CDC groups IIK-2 and IIb. Int J Syst Bacteriol 33:580–598 [CrossRef]
     [Google Scholar]
  33. Yoon J.-H., Lee S.-T., Kim S.-B., Kim W. Y., Goodfellow M., Park Y.-H. 1997; Restriction fragment length polymorphism analysis of PCR-amplified 16S ribosomal DNA for rapid identification of Saccharomonospora strains. Int J Syst Bacteriol 47:111–114 [CrossRef]
     [Google Scholar]
  34. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [CrossRef]
     [Google Scholar]
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Kaistella koreensis gen. nov., sp. nov., a novel member of the Chryseobacterium–Bergeyella–Riemerella branch
Int J Syst Evol Microbiol 54, 2319 (2004); https://doi.org/10.1099/ijs.0.02998-0
/content/journal/ijsem/10.1099/ijs.0.02998-0
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