1887

Abstract

A taxonomic study was performed on 26 strains isolated from microbial mats in Antarctic lakes of the Vestfold Hills and the McMurdo Dry Valleys. Phylogenetic analysis based on 16S rRNA gene sequences placed these strains within the group of the -subclass of the . Sequence similarity values for the strains with their nearest phylogenetic neighbours (, and ) ranged between 94·0 and 95·8 %. DNA–DNA hybridizations and comparison of repetitive extragenic palindromic DNA–PCR (rep-PCR) fingerprinting patterns revealed that these strains are members of three distinct species. The isolates are Gram-negative, chemoheterotrophic, non-motile rods and their DNA G+C contents range from 59·4 to 66·4 mol%. Whole-cell fatty acid profiles are similar and the primary fatty acid in all the strains is 18 : 1 7 (74·1–87·7 % of total). Genotypic results together with phenotypic characteristics allowed the differentiation of these species from related recognized species of the - and the strains are assigned to a new genus, gen. nov., with three novel species: sp. nov. (type species), consisting of ten strains with LMG 21507 (=CIP 108322) as type strain; sp. nov., consisting of 12 strains with LMG 22007 (=CIP 108323) as type strain; and sp. nov., consisting of four strains with LMG 22003 (=CIP 108321) as type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.03006-0
2004-07-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/4/ijs541263.html?itemId=/content/journal/ijsem/10.1099/ijs.0.03006-0&mimeType=html&fmt=ahah

References

  1. Barrow G. I., Feltham R. K. A. 1993 Cowan and Steel's Manual for the Identification of Medical Bacteria , 3rd edn. Cambridge: Cambridge University Press;
    [Google Scholar]
  2. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  3. González J. M., Kiene R. P., Moran M. A. 1999; Transformation of sulfur compounds by an abundant lineage of marine bacteria in the alpha-subclass of the class Proteobacteria. Appl Environ Microbiol 65:3810–3819
    [Google Scholar]
  4. González J. M., Simo R., Massana R., Covert J. S., Casamayor E. O., Pedros-Alio C., Moran M. A. 2000; Bacterial community structure associated with a dimethylsulfoniopropionate-producing North Atlantic algal bloom. Appl Environ Microbiol 66:4237–4246 [CrossRef]
    [Google Scholar]
  5. González J. M., Covert J. S., Whitman W. B. 8 other authors 2003; Silicibacter pomeroyi sp. nov. and Roseovarius nubinhibens sp. nov., dimethylsulfonioproprionate-demethylating bacteria from marine environments. Int J Syst Evol Microbiol 53:1261–1269 [CrossRef]
    [Google Scholar]
  6. Gosink J. J., Herwig R. P., Staley J. T. 1997; Octadecabacter arcticus gen. nov., sp. nov., and O. antarcticus sp. nov., nonpigmented, psychrophilic gas vacuolate bacteria from polar sea ice and water. Syst Appl Microbiol 20:356–365 [CrossRef]
    [Google Scholar]
  7. Hall T. A. 1999; bioedit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  8. Labrenz M., Collins M. D., Lawson P. A., Tindall B. J., Braker G., Hirsch P. 1998; Antarctobacter heliothermus gen. nov., sp. nov., a budding bacterium from hypersaline and heliothermal Ekho lake. Int J Syst Bacteriol 48:1363–1372 [CrossRef]
    [Google Scholar]
  9. Labrenz M., Collins M. D., Lawson P. A., Tindall B. J., Schumann P., Hirsch P. 1999; Roseovarius tolerans gen. nov., sp. nov., a budding bacterium with variable bacteriochlorophyll a production from hypersaline Ekho lake. Int J Syst Bacteriol 49:137–147 [CrossRef]
    [Google Scholar]
  10. Labrenz M., Tindall B. J., Lawson P. A., Collins M. D., Schumann P., Hirsch P. 2000; Staleya guttiformis gen. nov., sp. nov. and Sulfitobacter brevis sp. nov., α -3- Proteobacteria from hypersaline, heliothermal and meromictic antarctic Ekho lake. Int J Syst Evol Microbiol 50:303–313 [CrossRef]
    [Google Scholar]
  11. Lafay B., Ruimy R., Rausch de Traubenberg C., Breittmayer V., Gauthier M. J., Christen R. 1995; Roseobacter algicola sp. nov., a new marine bacterium isolated from the phycosphere of the toxin-producing dinoflagellate Prorocentrum lima . Int J Syst Bacteriol 45:290–296 [CrossRef]
    [Google Scholar]
  12. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  13. Pitcher D. G., Saunders N. A., Owen R. J. 1989; Rapid extraction of bacterial genomic DNA with guanidinium thiocyanate. Lett Appl Microbiol 8:151–156 [CrossRef]
    [Google Scholar]
  14. Pukall R., Buntefuß D., Frühling A., Rohde M, Kroppenstedt R. M., Burghardt J, Lebaron P, Bernard L, Stackebrandt E. 1999; Sulfitobacter mediterraneus sp. nov., a new sulfite-oxidizing member of the α - Proteobacteria . Int J Syst Bacteriol 49:513–519 [CrossRef]
    [Google Scholar]
  15. Rademaker J. L. W., de Bruijn F. J. 1997; Characterization and classification of microbes by rep-PCR genomic fingerprinting and computer assisted pattern analysis. In DNA Markers: Protocols, Applications and Overviews pp  151–171 Edited by Caetano-Anollés G., Gresshoff P. M. New York: Wiley;
    [Google Scholar]
  16. Rademaker J. L. W., Hoste B., Louws F. J., Kersters K., Swings J., Vauterin L., Vauterin P., de Bruijn F. J. 2000; Comparison of AFLP and rep-PCR genomic fingerprinting with DNA–DNA homology studies: Xanthomonas as a model system. Int J Syst Evol Microbiol 50:665–677 [CrossRef]
    [Google Scholar]
  17. Raes J., Van de Peer Y. 1999 ForCon: a software tool for the conversion of sequence alignments ; http://www.ebi.ac.uk/embnet.news/vol6_1/ForCon/body_forcon.html
    [Google Scholar]
  18. Reichenbach H., Dworkin M. 1981; Introduction to the gliding bacteria. In The Prokaryotes vol 1 pp  315–327 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. Berlin: Springer;
    [Google Scholar]
  19. Ruiz-Ponte C., Cilia V., Lambert C., Nicolas J. L. 1998; Roseobacter gallaeciensis sp. nov., a new marine bacterium isolated from rearings and collectors of the scallop Pecten maximus . Int J Syst Bacteriol 48:537–542 [CrossRef]
    [Google Scholar]
  20. Saitou N., Nei M. 1987; The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  21. Shiba T. 1991; Roseobacter litoralis gen. nov., sp. nov., and Roseobacter denitrificans sp. nov., aerobic pink-pigmented bacteria which contain bacteriochlorophyll a . Syst Appl Microbiol 14:140–145 [CrossRef]
    [Google Scholar]
  22. Urbance J. W., Bratina B. J., Stoddard S. F., Schmidt T. M. 2001; Taxonomic characterization of Ketogulonigenium vulgare gen.nov., sp. nov. and Ketogulonigenium robustum sp. nov., which oxidize l-sorbose to 2-keto-l-gulonic acid. Int J Syst Evol Microbiol 51:1059–1070 [CrossRef]
    [Google Scholar]
  23. Van de Peer Y., De Wachter R. 1994; treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10:569–570
    [Google Scholar]
  24. Van Trappen S., Mergaert J., Van Eygen S., Dawyndt P., Cnockaert M. C., Swings J. 2002; Diversity of 746 heterotrophic bacteria isolated from microbial mats from ten Antarctic lakes. Syst Appl Microbiol 25:603–610 [CrossRef]
    [Google Scholar]
  25. Van Trappen S., Mergaert J., Swings J. 2003; Flavobacterium gelidilacus sp. nov., isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 53:1241–1245 [CrossRef]
    [Google Scholar]
  26. Van Trappen S., Vandecandelaere I., Mergaert J., Swings J. 2004; Flavobacterium degerlachei sp. nov., Flavobacterium frigoris sp.nov. and Flavobacterium micromati sp. nov., novel psychrophilic bacteria isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 54:85–92 [CrossRef]
    [Google Scholar]
  27. Versalovic J., Koeuth T., Lupski J. R. 1991; Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting in bacterial genomes. Nucleic Acids Res 19:6823–6831 [CrossRef]
    [Google Scholar]
  28. Versalovic J., Schneider M., de Bruijn F. J., Lupski J. R. 1994; Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Methods Mol Cell Biol 5:25–40
    [Google Scholar]
  29. Wagner-Döbler I., Rheims H., Felske A., Pukall R., Tindall B. J. 2003; Jannaschia helgolandensis gen. nov., sp. nov., a novel abundant member of the marine Roseobacter clade from the North Sea. Int J Syst Evol Microbiol 53:731–738 [CrossRef]
    [Google Scholar]
  30. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.03006-0
Loading
/content/journal/ijsem/10.1099/ijs.0.03006-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error