1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 62, Issue 2

sp. nov., isolated from seawater Free

Abstract

A Gram-negative, pink-pigmented, non-motile, strictly aerobic rod, designated CNU040, was isolated from seawater from the coast of Jeju Island in Korea. The temperature, pH and NaCl ranges for growth were 4–30 °C, pH 5.5–10.0 and 0–5.0 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CNU040 belonged to a distinct lineage in the genus and exhibited high sequence similarity with DS-44 (98.3 %) and AC-74 (96.6 %) and lower sequence similarity (<96.0 %) with all other members of the genus DNA–DNA relatedness between strain CNU040 and KCTC 12545 was 44.5 %. The DNA G+C content of the isolate was 48.5 mol% and the major respiratory quinone was menaquinone-7. The major cellular fatty acids were iso-C (28.6 %) and summed feature 3 (consisting of iso-C 2-OH and/or Cω7; 24.0 %). The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, one unknown amino lipid, one unknown aminophospholipid and three unknown polar lipids. On the basis of phenotypic, phylogenetic and genotypic data, strain CNU040 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is CNU040 ( = KCTC 22647  = JCM 16112).

  • Published Online:
Funding
This study was supported by the:
  • 21C Frontier Microbial Genomics and Applications Centre Program, Ministry of Education, Science and Technology, Korea
© 2012 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.030106-0
2012-02-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/2/409.html?itemId=/content/journal/ijsem/10.1099/ijs.0.030106-0&mimeType=html&fmt=ahah

References

  1. Baumann P., Baumann L. 1981; The marine gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas and Alcaligenes . In The Prokaryotes vol. 1 pp. 1302–1331 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. Berlin: Springer;
     [Google Scholar]
  2. Bernardet J.-F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [View Article][PubMed]
     [Google Scholar]
  3. Bowman J. P., Nichols C. M., Gibson J. A. E. 2003; Algoriphagus ratkowskyi gen. nov., sp. nov., Brumimicrobium glaciale gen. nov., sp. nov., Cryomorpha ignava gen. nov., sp. nov. and Crocinitomix catalasitica gen. nov., sp. nov., novel flavobacteria isolated from various polar habitats. Int J Syst Evol Microbiol 53:1343–1355 [View Article][PubMed]
     [Google Scholar]
  4. Brown T. 2005; Dot and slot blotting of DNA onto a positively charged nylon membrane using a manifold. In Current Protocols in Molecular Biology pp. 2.9.18–2.9.20 Edited by Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. New York: Wiley;
     [Google Scholar]
  5. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y.-W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
     [Google Scholar]
  6. CLSI 2003; Performance standards for antimicrobial disk susceptibility tests, 8th edn. Approved Standard M2-A8. Wayne, PA: Clinical and Laboratory Standards Institute;
  7. Copa-Patiño J. L., Arenas M., Soliveri J., Sánchez-Porro C., Ventosa A. 2008; Algoriphagus hitonicola sp. nov., isolated from an athalassohaline lagoon. Int J Syst Evol Microbiol 58:424–428 [View Article][PubMed]
     [Google Scholar]
  8. Felsenstein J. 1985; Confidence limit on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
     [Google Scholar]
  9. Hall T. 2007 BioEdit. Biological sequence alignment editor for Win95/98/NT/2K/XP Carlsbad, CA: Ibis Biosciences;
     [Google Scholar]
  10. Johnson J. L. 1994; Similarity analysis of rRNAs. In Methods for General and Molecular Bacteriology pp. 683–700 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  11. Kimura M. 1983 The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press; [CrossRef]
     [Google Scholar]
  12. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
     [Google Scholar]
  13. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67 [View Article]
     [Google Scholar]
  14. Liu Y., Li H., Jiang J.-T., Liu Y.-H., Song X.-F., Xu C.-J., Liu Z.-P. 2009; Algoriphagus aquatilis sp. nov., isolated from a freshwater lake. Int J Syst Evol Microbiol 59:1759–1763 [View Article][PubMed]
     [Google Scholar]
  15. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [View Article][PubMed]
     [Google Scholar]
  16. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
     [Google Scholar]
  17. Nedashkovskaya O. I., Vancanneyt M., Van Trappen S., Vandemeulebroecke K., Lysenko A. M., Rohde M., Falsen E., Frolova G. M., Mikhailov V. V., Swings J. 2004; Description of Algoriphagus aquimarinus sp. nov., Algoriphagus chordae sp. nov. and Algoriphagus winogradskyi sp. nov., from sea water and algae, transfer of Hongiella halophila Yi and Chun 2004 to the genus Algoriphagus as Algoriphagus halophilus comb. nov. and emended descriptions of the genera Algoriphagus Bowman et al. 2003 and Hongiella Yi and Chun 2004. Int J Syst Evol Microbiol 54:1757–1764 [View Article][PubMed]
     [Google Scholar]
  18. Nedashkovskaya O. I., Kim S. B., Kwon K. K., Shin D. S., Luo X., Kim S.-J., Mikhailov V. V. 2007; Proposal of Algoriphagus vanfongensis sp. nov., transfer of members of the genera Hongiella Yi and Chun 2004 emend. Nedashkovskaya et al. 2004 and Chimaereicella Tiago et al. 2006 to the genus Algoriphagus, and emended description of the genus Algoriphagus Bowman et al. 2003 emend. Nedashkovskaya et al. 2004. Int J Syst Evol Microbiol 57:1988–1994 [View Article][PubMed]
     [Google Scholar]
  19. Park S., Kang S.-J., Oh K.-H., Oh T.-K., Yoon J.-H. 2010; Algoriphagus lutimaris sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 60:200–204 [View Article][PubMed]
     [Google Scholar]
  20. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc;
  21. Smibert R. M., Krieg N. R. 1994; Phenotypic characteristics. In Methods for General and Molecular Biology pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  22. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
     [Google Scholar]
  23. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal _ x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
     [Google Scholar]
  24. Tiago I., Mendes V., Pires C., Morais P. V., Veríssimo A. 2006; Chimaereicella alkaliphila gen. nov., sp. nov., a Gram-negative alkaliphilic bacterium isolated from a nonsaline alkaline groundwater. Syst Appl Microbiol 29:100–108 [View Article][PubMed]
     [Google Scholar]
  25. Tindall B. J., Sikorski J., Smibert R. A., Krieg N. R. 2007; Phenotypic characterization and principles of comparative systematics. In Methods for General and Molecular Microbiology pp. 330–393 Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G. A., Schmidt T. M., Snyder L. R. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  26. Van Trappen S., Vandecandelaere I., Mergaert J., Swings J. 2004; Algoriphagus antarcticus sp. nov., a novel psychrophile from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 54:1969–1973 [View Article][PubMed]
     [Google Scholar]
  27. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
     [Google Scholar]
  28. Yi H., Chun J. 2004; Hongiella mannitolivorans gen. nov., sp. nov., Hongiella halophila sp. nov. and Hongiella ornithinivorans sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 54:157–162 [View Article][PubMed]
     [Google Scholar]
  29. Yoon J.-H., Kang S.-J., Jung S.-Y., Lee C.-H., Oh T.-K. 2005a; Algoriphagus yeomjeoni sp. nov., isolated from a marine solar saltern in the Yellow Sea, Korea. Int J Syst Evol Microbiol 55:865–870 [View Article][PubMed]
     [Google Scholar]
  30. Yoon J.-H., Kang S.-J., Oh T.-K. 2005b; Algoriphagus locisalis sp. nov., isolated from a marine solar saltern. Int J Syst Evol Microbiol 55:1635–1639 [View Article][PubMed]
     [Google Scholar]
  31. Yoon J.-H., Lee M.-H., Kang S.-J., Oh T.-K. 2006; Algoriphagus terrigena sp. nov., isolated from soil. Int J Syst Evol Microbiol 56:777–780 [View Article][PubMed]
     [Google Scholar]
  32. Young C.-C., Lin S.-Y., Arun A. B., Shen F.-T., Chen W.-M., Rekha P. D., Langer S., Busse H.-J., Wu Y.-H., Kämpfer P. 2009; Algoriphagus olei sp. nov., isolated from oil-contaminated soil. Int J Syst Evol Microbiol 59:2909–2915 [View Article][PubMed]
     [Google Scholar]
  33. ZoBell C. E. 1941; Studies on marine bacteria. I. The cultural requirements of heterotrophic aerobes. J Mar Res 4:42–75
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.030106-0
Loading
Algoriphagus jejuensis sp. nov., isolated from seawater
Int J Syst Evol Microbiol 62, 409 (2012); https://doi.org/10.1099/ijs.0.030106-0
/content/journal/ijsem/10.1099/ijs.0.030106-0
/content/journal/ijsem/10.1099/ijs.0.030106-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error