1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 54, Issue 5

gen. nov., sp. nov., a novel actinomycete with -ornithine and -serine in the peptidoglycan Free

Abstract

A Gram-positive bacterial strain containing -ornithine as the diagnostic diamino acid was isolated from a sea-water-sample from the East Sea, Korea. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain JC1078 represents a phyletic line within the suborder of the order , adjacent to the genus . The highest sequence similarity values to the isolate were observed against (94·3 %) and (94·1 %). The strain was strictly aerobic and moderately halophilic with optimal growth at 2–3 % (w/v) NaCl. Cells were non-motile, non-sporulating and coccoid-shaped. The cell wall contains -ornithine, glutamic acid, alanine, glycine and serine. The major menaquinone was MK-8(H). The predominant cellular fatty acids were of the iso- and anteiso-methyl-branched types. The polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and an unknown glycolipid. The acyl type of the glycan chain of peptidoglycan is acetyl. The DNA G+C content was 72 mol%. The combination of physiological, biochemical and chemotaxonomical data clearly separated the marine isolate from other members of the suborder . On the basis of polyphasic evidence, it is proposed to classify strain JC1078 in a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain is JC1078 (=IMSNU 14026=KCTC 9980=DSM 15273).

  • Published Online:
SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.03036-0
2004-09-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/5/ijs541585.html?itemId=/content/journal/ijsem/10.1099/ijs.0.03036-0&mimeType=html&fmt=ahah

References

  1. Baumann L., Baumann P., Mandel M., Allen R. D. 1972; Taxonomy of aerobic marine eubacteria. J Bacteriol 110:402–429
     [Google Scholar]
  2. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [CrossRef]
     [Google Scholar]
  3. Chun J., Goodfellow M. 1995; A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45:240–245 [CrossRef]
     [Google Scholar]
  4. Chun J., Bae K. S., Moon E. Y., Jung S. O., Lee H. K., Kim S. J. 2000; Nocardiopsis kunsanensis sp. nov., a moderately halophilic actinomycete isolated from a saltern. Int J Syst Evol Microbiol 50:1909–1913
     [Google Scholar]
  5. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [CrossRef]
     [Google Scholar]
  6. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
     [Google Scholar]
  7. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
     [Google Scholar]
  8. Fitch W. M. 1972; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416
     [Google Scholar]
  9. Fitch W. M., Margoliash E. 1967; Construction of phylogenetic trees. Science 155:279–284 [CrossRef]
     [Google Scholar]
  10. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. 1974; Nocardia coeliaca , Nocardia autotrophica , and the nocardin strain. Int J Sys Bacteriol 24:54–63 [CrossRef]
     [Google Scholar]
  11. Gosink J. J., Woese C. R., Staley J. T. 1998; Polaribacter gen. nov., with three new species, P.irgensii sp. nov., P. franzmannii sp.nov. and P. filamentus sp. nov.,gas vacuolate polar marine bacteria of the Cytophaga – Flavobacterium – Bacteroides group and reclassification of ‘ Flectobacillus glomeratus ’ as Polaribacter glomeratus comb. nov. Int J Syst Bacteriol 48:223–235 [CrossRef]
     [Google Scholar]
  12. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. 1996; Agrococcus jenensis gen. nov., sp. nov. a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239 [CrossRef]
     [Google Scholar]
  13. Groth I., Schumann P., Martin K., Schuetze B., Augsten K., Kramer I., Stackebrandt E. 1999; Ornithinicoccus hortensis gen. nov., sp. nov., a soil actinomycete which contains l-ornithine. Int J Syst Bacteriol 49:1717–1724 [CrossRef]
     [Google Scholar]
  14. Groth I., Schumann P., Weiss N., Schuetze B., Augsten K., Stackebrandt E. 2001; Ornithinimicrobium humiphilum gen. nov., sp. nov. a novel soil actinomycete with l-ornithine in the peptidoglycan. Int J Syst Evol Microbiol 51:81–87
     [Google Scholar]
  15. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp  21–132 Edited by Munro H. N. New York: Academic Press;
     [Google Scholar]
  16. Lyman J., Fleming R. H. 1940; Composition of sea water. J Mar Res 3:134–146
     [Google Scholar]
  17. MacKenzie S. L. 1987; Gas chromatographic analysis of amino acids as the N -heptafluorobutyryl isobutyl esters. J Assoc Off Anal Chem 70:151–160
     [Google Scholar]
  18. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
     [Google Scholar]
  19. MIDI 1999; Sherlock, Microbial Identification System, Operating Manual, version 3.0. Newark, DE: MIDI, Inc;
     [Google Scholar]
  20. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
     [Google Scholar]
  21. Schleifer K. H. 1985; Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156
     [Google Scholar]
  22. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
     [Google Scholar]
  23. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–654 Washington, DC: American Society for Microbiology;
     [Google Scholar]
  24. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491 [CrossRef]
     [Google Scholar]
  25. Staley J. T. 1968; Prosthecomicrobium and Ancalomicrobium : new prosthecate freshwater bacteria. J Bacteriol 95:1921–1942
     [Google Scholar]
  26. Swofford D. L. 1998 Phylogenetic Analysis Using Parsimony (PAUP) . Version 4 Sunderland, MA: Sinauer Associates;
     [Google Scholar]
  27. Uchida K., Aida K. 1984; An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. J Gen Appl Microbiol 30:131–134 [CrossRef]
     [Google Scholar]
  28. Zobell C. E. 1941; Studies on marine bacteria. I. The cultural requirements of heterotrophic aerobes. J Mar Res 4:42–75
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.03036-0
Loading
Serinicoccus marinus gen. nov., sp. nov., a novel actinomycete with l-ornithine and l-serine in the peptidoglycan
Int J Syst Evol Microbiol 54, 1585 (2004); https://doi.org/10.1099/ijs.0.03036-0
/content/journal/ijsem/10.1099/ijs.0.03036-0
/content/journal/ijsem/10.1099/ijs.0.03036-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error