1887

Abstract

Two Gram-negative, oxidase-positive rods (strains BSB 9.5 and BSB 41.8) isolated from wastewater were studied using a polyphasic approach. 16S rRNA gene sequence comparisons demonstrated that both strains cluster phylogenetically within the family : the two strains shared 99·9 % 16S rRNA gene sequence similarity and were most closely related to the type strains of (98·5 %) and (98·0 %). The fatty acid patterns and substrate-utilization profiles displayed similarity to the those of the five species with validly published names, although clear differentiating characteristics were also observed. The two strains showed DNA–DNA hybridization values of 51 % with respect to each other. No close similarities to any other species were detected in hybridization experiments with the genomic DNAs. On the basis of these results, two novel species, sp. nov. and sp. nov. are proposed, with BSB 9.5 (=DSM 15341=CIP 108119) and BSB 41.8 (=DSM 15342=CIP 108118) as the respective type strains.

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2005-01-01
2024-03-28
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