1887

Abstract

A novel mesophilic sulfur- and thiosulfate-oxidizing bacterium, strain 42BKT, was isolated from the gas-bubbling sediment at the Iheya North hydrothermal system in the mid-Okinawa Trough, Japan. The isolate was a Gram-negative, non-motile and coccoid to oval-shaped bacterium. Growth was observed at 10–40 °C (optimum 28–30 °C) and in the pH range 5·0–9·0 (optimum 6·5–7·0). Strain 42BKT grew chemolithoautotrophically with elemental sulfur or thiosulfate as a sole electron donor and oxygen (optimum 5 % in gas phase) or nitrate as an electron acceptor. The G+C content of the genomic DNA was 48·0 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the isolate belonged to the previously uncultivated Group F within the -, which includes phylotypes of vent epibiont and environmental sequences from global deep-sea cold seep and hydrothermal vent fields. On the basis of the physiological and molecular characteristics of this isolate, the type species of a novel genus, gen. nov., sp. nov., is proposed. The type strain is 42BKT (=ATCC BAA-797=JCM 12117).

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2004-09-01
2024-03-28
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References

  1. Alain K., Querellou J., Lesongeur F., Pignet P., Crassous P., Raguénès G., Cueff V., Cambon-Bonavita M.-A. 2002; Caminibacter hydrogeniphilus gen. nov., sp. nov. a novel thermophilic, hydrogen-oxidizing bacterium isolated from an East Pacific Rise hydrothermal vent. Int J Syst Evol Microbiol 52:1317–1323 [CrossRef]
    [Google Scholar]
  2. Balch W. E., Fox G. E., Magrum L. J., Woese C. R., Wolfe R. S. 1979; Methanogens: reevaluation of a unique biological group. Microbiol Rev 43:260–296
    [Google Scholar]
  3. Baross J. A. 1995; Isolation, growth and maintenance of hyperthermophiles. In Archaea: a Laboratory Manual Thermophiles pp  15–23 Edited by Robb F. T., R A. Place. Cold Springer; Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  4. Campbell B. J., Jeanthon C., Kostka J. E., Luther G. W. III, Cary S. C. 2001; Growth and phylogenetic properties of novel bacteria belonging to the epsilon subdivision of the Proteobacteria enriched from Alvinella pompejana and deep-sea hydrothermal vents. Appl Environ Microbiol 67:4566–4572 [CrossRef]
    [Google Scholar]
  5. Corre E., Reysenbach A.-L., Prieur D. 2001; Epsilon-proteobacterial diversity from a deep-sea hydrothermal vent on the Mid-Atlantic Ridge. FEMS Microbiol Lett 205:329–335
    [Google Scholar]
  6. Inagaki F., Sakihama Y., Inoue A., Kato C., Horikoshi K. 2002; Molecular phylogenetic analyses of reverse-transcribed bacterial rRNA obtained from deep-sea cold seep sediments. Environ Microbiol 4:277–286 [CrossRef]
    [Google Scholar]
  7. Inagaki F., Takai K., Nealson K. H., Horikoshi K. 2003; Sulfurimonas autotrophica gen. nov., sp. nov., a novel sulfur-oxidizing epsilon-proteobacterium isolated from hydrothermal sediments in the mid-Okinawa Trough. Int J Syst Evol Microbiol 53:1801–1805 [CrossRef]
    [Google Scholar]
  8. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  9. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  10. Li L., Guezennec J., Nichols P., Henry P., Yanagibayashi M., Kato C. 1999; Microbial diversity in Nankai Trough sediments at a depth of 3,843m. J Oceanogr 55:635–642 [CrossRef]
    [Google Scholar]
  11. Longnecker K., Reysenbach A.-L. 2001; Expansion of the geographic distribution of a novel lineage of epsilon-Proteobacteria to a hydrothermal vent site on the Southern East Pacific Rise. FEMS Microbiol Lett 35:287–293
    [Google Scholar]
  12. Miroshnichenko M. L., Kostrikina N. A., L'Haridon S., Jeanthon C., Hippe H., Stackebrandt E., Bonch-Osmolovskaya E. A. 2002; Nautilia lithotrophica gen. nov., sp. nov. a thermophilic sulfur-reducing ε -proteobacterium isolated from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 52:1299–1304 [CrossRef]
    [Google Scholar]
  13. Moyer C. L., Dobbs F. C., Karl D. M. 1995; Phylogenetic diversity of the bacterial community from a microbial mat at an active, hydrothermal vent system, Loihi Seamount. Hawaii. Appl Environ Microbiol 61:1555–1562
    [Google Scholar]
  14. Polz M. F., Cavanaugh C. M. 1995; Dominance of one bacterial phylotype at a Mid-Atlantic Ridge hydrothermal vent site. Proc Natl Acad Sci U S A 92:7232–7236 [CrossRef]
    [Google Scholar]
  15. Porter K. G., Feig Y. S. 1980; The use of DAPI for identifying and counting microflora. Limnol Oceanogr 25:943–948 [CrossRef]
    [Google Scholar]
  16. Reysenbach A. L., Longnecker K., Kirshtein J. 2000; Novel bacterial and archaeal lineages from an in situ growth chamber deployed at a Mid-Atlantic Ridge hydrothermal vent. Appl Environ Microbiol 66:3798–3806 [CrossRef]
    [Google Scholar]
  17. Takai K., Inagaki F., Nakagawa S., Hirayama H., Nunoura T., Sako Y., Nealson K. H., Horikoshi K. 2003; Isolation and phylogenetic diversity of members of previously uncultivated epsilon-Proteobacteria in deep-sea hydrothermal fields. FEMS Microbiol Lett 218:167–174
    [Google Scholar]
  18. Takai K., Nealson K. H., Horikoshi K. 2004; Hydrogenimonas thermophila gen. nov., sp. nov., a novel thermophilic, hydrogen-oxidizing chemolithoautotroph within the ε - Proteobacteria isolated from a black smoker in a Central Indian Ridge hydrothermal field. Int J Syst Evol Microbiol 54:25–32 [CrossRef]
    [Google Scholar]
  19. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  20. Teske A., Hinrichs K. U., Edgcomb V., de Vera Gomez A., Kysela D., Sylva S. P., Sogin M. L., Jannasch H. W. 2002; Microbial diversity of hydrothermal sediments in the Guaymas Basin: evidence for anaerobic methanotrophic communities. Appl Environ Microbiol 68:1994–2007 [CrossRef]
    [Google Scholar]
  21. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  22. Zillig W., Holz I., Janekovic D. 7 other authors 1990; Hyperthermus butylicus , a hyperthermophilic sulfur-reducing archaebacterium that ferments peptides. J Bacteriol 172:3959–3965
    [Google Scholar]
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