RT Journal Article SR Electronic(1) A1 Jabari, Linda A1 Gannoun, Hana A1 Cayol, Jean-Luc A1 Hamdi, Moktar A1 Fauque, Guy A1 Ollivier, Bernard A1 Fardeau, Marie-LaureYR 2012 T1 Characterization of Defluviitalea saccharophila gen. nov., sp. nov., a thermophilic bacterium isolated from an upflow anaerobic filter treating abattoir wastewaters, and proposal of Defluviitaleaceae fam. nov. JF International Journal of Systematic and Evolutionary Microbiology, VO 62 IS Pt_3 SP 550 OP 555 DO https://doi.org/10.1099/ijs.0.030700-0 PB Microbiology Society, SN 1466-5034, AB A novel thermophilic, anaerobic, Gram-stain-positive, terminal-spore-forming bacterium was isolated from an upflow anaerobic filter treating abattoir wastewaters in Tunisia. This strain, designated LIND6LT2T, grew at 40–60 °C (optimum 50–55 °C) and at pH 6.0–8.5 (optimum pH 7.0–7.5). It did not require NaCl for growth, but tolerated it up to 2 %. Sulfate, thiosulfate, elemental sulfur, sulfite, nitrate and nitrite were not used as electron acceptors. Growth of LIND6LT2T was inhibited by sulfite (2 mM). Strain LIND6LT2T used cellobiose, glucose, mannose, maltose, mannitol, sucrose and xylose as electron donors. The main fermentation products from glucose metabolism were acetate, formate, butyrate and isobutyrate. The predominant cellular fatty acids were C16 : 0 (68.4 %) and C14 : 0 (8.3 %). The G+C content of the genomic DNA was 35.2 mol%. On the basis of its phylogenetic and physiological properties, a new genus and species, Defluviitalea saccharophila gen. nov., sp. nov., are proposed to accommodate strain LIND6LT2T, placed in Defluviitaleaceae fam. nov. within the phylum Firmicutes, class Clostridia, order Clostridiales. Strain LIND6LT2T ( = DSM 22681T  = JCM 16312T) is the type strain of Defluviitalea saccharophila, which itself is the type species of Defluviitalea., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.030700-0